INT88390

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Context Info
Confidence 0.69
First Reported 2000
Last Reported 2010
Negated 1
Speculated 1
Reported most in Body
Documents 27
Total Number 28
Disease Relevance 12.28
Pain Relevance 4.15

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (LGALS4) cell adhesion (LGALS4) plasma membrane (LGALS4)
Anatomy Link Frequency
T cell 6
lamina 2
HT-29 1
Caco-2 1
IB4 1
LGALS4 (Homo sapiens)
Pain Link Frequency Relevance Heat
Adalimumab 48 97.72 Very High Very High Very High
Inflammation 889 96.64 Very High Very High Very High
Spinal cord 1 95.12 Very High Very High Very High
Potency 24 91.20 High High
abdominal pain 24 89.08 High High
cytokine 672 88.88 High High
cINOD 24 87.40 High High
Peripheral nerve injury 1 82.64 Quite High
Inflammatory response 24 76.12 Quite High
Crohn's disease 96 72.92 Quite High
Disease Link Frequency Relevance Heat
Nociception 4 100.00 Very High Very High Very High
Colitis 408 99.56 Very High Very High Very High
Adenocarcinoma 67 98.88 Very High Very High Very High
Apoptosis 1296 98.68 Very High Very High Very High
Death 482 96.68 Very High Very High Very High
INFLAMMATION 649 96.64 Very High Very High Very High
Cancer 89 93.88 High High
Amebiasis 1 93.76 High High
Necrosis 72 90.48 High High
Abdominal Pain 24 89.08 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Interestingly, in contrast to the lack of Gal-4 antisense to interfere with the T cell cycle, blockade of Gal-4 expression increased TNF-?
Gene_expression (expression) of Gal-4 in T cell
1) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0 Pain Relevance 0.19
Whereas it is known that in the porcine and murine small intestine Gal-4 is expressed in epithelial cells [7], its expression pattern in the human GI tract remains unknown.
Gene_expression (expressed) of Gal-4 in small intestine
2) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.81 Pain Relevance 0.27
Having defined that Gal-4 is strongly expressed in gastrointestinal epithelial cells, we next investigated, if Gal-4 is also secreted by epithelial cells and thus can interact with the underlying T cells in the lamina propria.


Gene_expression (expressed) of Gal-4 in lamina
3) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.28 Pain Relevance 0.06
Indeed, blockade of Gal-4 expression by specific antisense oligonucleotides modestly reduced adalimumab-induced T cell death from 63% of cells to 53% (Fig 5C).
Gene_expression (expression) of Gal-4 in T cell associated with adalimumab and death
4) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.93 Pain Relevance 0.08
Gal-4 expression was inhibited by 49±7%.
Gene_expression (expression) of Gal-4
5) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0 Pain Relevance 0.19
In contrast, only weak Gal-4 positivity was detected in the underlying lamina propria cells (Fig. 1A) and absent in liver and spleen (not shown).
Neg (absent) Gene_expression (detected) of Gal-4 in lamina
6) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.66 Pain Relevance 0.22
By performing sequential immunohistochemical analysis, we showed strong Gal-4 expression in epithelial cells along the alimentary tract and thus confirmed the previously described RNA expression profile [7].
Gene_expression (expression) of Gal-4 in alimentary tract
7) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.45 Pain Relevance 0.15
Our results systematically and sequentially uncovered the expression and secretion profile of Gal-4 as well as a previously unrecognized biological function of this lectin.
Gene_expression (expression) of Gal-4
8) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 1.44 Pain Relevance 0.41
Expression of Gal-4 in T cells
Gene_expression (Expression) of Gal-4 in T cells
9) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.85 Pain Relevance 0.29
Immunohistochemistry for Gal-4 expression
Gene_expression (expression) of Gal-4
10) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.47 Pain Relevance 0.21
Levels of Gal-4 were determined in supernatants of HT-29 and Caco-2 cells cultured for 48 h.
Gene_expression (Levels) of Gal-4 in HT-29
11) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.24 Pain Relevance 0.10
Expression of Gal-4 was revealed using a goat anti-human Gal-4 polyclonal Ab (R&D Systems) followed by a biotinylated anti-goat-Ab (Vector laboratories).
Gene_expression (Expression) of Gal-4
12) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.26 Pain Relevance 0.14
Therefore, we down-regulated Gal-4 expression with specific antisense oligonucleotides.
Gene_expression (expression) of Gal-4
13) Confidence 0.69 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0 Pain Relevance 0
When the Gal-4 expression was quantified on the protein level, Gal-4 was detected intra- and extracellularly in PBMC, regardless of their activation status (Fig. 1C).
Gene_expression (expression) of Gal-4
14) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.42 Pain Relevance 0.12
In contrast, the tandem-repeat type Gal-4 is exclusively expressed within the GI tract which suggests that Gal-4 might play a more defined and selective role within the intestinal mucosa, prompting us to investigate the role of Gal-4 in mucosal inflammatory processes.
Gene_expression (expressed) of Gal-4 associated with inflammation
15) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.53 Pain Relevance 0.30
As verified by TaqMan real time PCR, Gal-4 expression was inhibited by the specific antisense oligonucleotides by 49±7%.
Gene_expression (expression) of Gal-4
16) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0 Pain Relevance 0
Using the same experimental design as above, we transfected PBT with specific Gal-4 antisense to investigate the role of endogenous Gal-4.
Spec (investigate) Gene_expression (transfected) of Gal-4
17) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0 Pain Relevance 0.20
Gal-4 was detected by ELISA using an anti-human Galectin-4 capture Ab (R&D Systems) and a biotinylated anti-human Galectin-4 Ab (R&D Systems) and visualized by streptavidin-horseradish peroxidase and TMB substrate (both from BD Pharmingen).


Gene_expression (detected) of Gal-4
18) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.19 Pain Relevance 0.10
Uncovering the involvement of Gal-4 in the apoptotic program of T cells and assigning a new biological role to endogenous Gal-4, in deed, inhibition of Gal-4 expression by specific antisense oligonucleotides decreased TNF-?
Gene_expression (expression) of Gal-4 in T cells associated with apoptosis
19) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 1.25 Pain Relevance 0.14
When the entire human GI tract was sequentially characterized by immunohistochemical staining, strong Gal-4 staining was observed in epithelial cells of the antrum, ileum, colon and rectum (Fig. 1A) as well as in the corpus, fundus, and jejunum (not shown).
Gene_expression (staining) of Gal-4 in epithelial cells
20) Confidence 0.60 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2440804 Disease Relevance 0.73 Pain Relevance 0.24

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