INT88721
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
(Vandesompele et al., 2002) At least three out of the following four housekeeping genes were used for each experiment: glyceraldehyde-3 phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1), human porphobilinogen deaminase (PBGD) and TATA box binding protein (TBP). | |||||||||||||||
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(Vandesompele et al., 2002) At least three out of the following four housekeeping genes were used for each experiment: glyceraldehyde-3 phosphate dehydrogenase (GAPDH), hypoxanthine phosphoribosyltransferase 1 (HPRT1), human porphobilinogen deaminase (PBGD) and TATA box binding protein (TBP). | |||||||||||||||
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For normalization of the data the average of the expression level of four house-keeping genes, GAPDH, HPRT, beta-actin and beta-tubulin, was used.
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mRNA and hypoxanthine phosphoribosyltransferase (HPRT) mRNA expression was quantified using QRTPCR. | |||||||||||||||
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Here we show that infection of human 1306, HPRT-negative cells with RAd-HPRT, expressed high enough levels of HPRT enzyme activity, as to reverse their abnormal biochemical phenotype, thus enhancing hypoxanthine incorporation and restoring purine recycling, increasing GTP levels, decreasing adenine incorporation, and allowing cell survival in HAT medium in which only cells expressing high levels of HPRT can survive. | |||||||||||||||
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The HPRT1 transcript was selected as the reference gene on an empirical basisHPRT1 expression in these men (1863±111 arbitrary units, mean±SEM) and women (1881±138 arbitrary units) was similar according to the microarray data (this choice does not necessarily mean that HPRT1 expression always is constant in muscle). | |||||||||||||||
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Since gene expression quantification using RT-qPCR requires a steady reference gene, we selected three genes frequently used for normalization of the data, ABL1, RPLP0, and HPRT1. | |||||||||||||||
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