INT88789
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Co-administration of morphine twice daily and PSD-95 antisense oligodeoxynucleotide (50 microg/10 microl) once daily for 4 days not only markedly reduced the PSD-95 expression and its binding to NMDA receptors in spinal cord but also significantly prevented the development of morphine tolerance. | |||||||||||||||
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OBJECTIVE: To observe the expression of brain-derived neurotrophic factor (BDNF) and postsynaptic density-95 (PSD-95) in hippocampal CA1 region of rat with morphine dependence for different times and withdrawn for 1 week, and investigate the influence of that morphine dependence is withdrawn on rat hippocampal CA1 area. | |||||||||||||||
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RESULTS: The expression of BDNF and PSD-95 in hippocampal CA1 decreased in the withdrawn group with morphine dependence for 1 week as compared with that in normal saline (NS) group (P < 0.01), and it increased in the withdrawn group with morphine dependence for 2 weeks as compared with that in morphine-dependent group for 1 week (P < 0.05) but still decreased as compared with that in NS group (P < 0.01), and it decreased in the withdrawn group with morphine dependence for 4 weeks as compared with the other three groups (P < 0.01). | |||||||||||||||
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[The expression of BDNF and PSD-95 in hippocampal CA1 region of morphine-withdrawn rat with different dependent times]. | |||||||||||||||
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The expression of BDNF and PSD-95 in hippocampal CA1 were identified with RT-PCR. | |||||||||||||||
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CONCLUSION: The expression of BDNF and PSD-95 in hippocampal CA1 decreases in morphine-depended rats withdrawn for 1 week. | |||||||||||||||
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OBJECTIVE: To observe the expression of brain-derived neurotrophic factor (BDNF) and postsynaptic density-95 (PSD-95) in hippocampal CA1 region of rat with morphine dependence for different times and withdrawn for 1 week, and investigate the influence of that morphine dependence is withdrawn on rat hippocampal CA1 area. | |||||||||||||||
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It was thus necessary to verify that the activity recorded through the electrodes faithfully represented the activity of those neurons expressing PSD-95:GFP and followed by time-lapse microscopy. | |||||||||||||||
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PSDs were visualized by expressing an EGFP-tagged variant of the PSD molecule PSD-95 (PSD-95:GFP). | |||||||||||||||
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Furthermore, due to the random nature of lentiviral infection, neurons expressing PSD-95:GFP were not necessarily located over any particular electrode. | |||||||||||||||
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For example, in the first study mentioned above, PSD-95:GFP expression levels were reported to be many fold greater than endogenous PSD-95 levels, whereas the use of Sindbis or Semliki forest viral vectors for PSD-95:GFP expression in others might have resulted in similar situations [72]. | |||||||||||||||
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Furthermore, PSD-95:GFP overexpression was previously shown to affect synaptic properties and even occlude forms of activity-induced synaptic plasticity [67][71]. | |||||||||||||||
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Given the low PSD-95:GFP expression levels here and the fact that the aforementioned forms of synaptic plasticity were not occluded in our system, it seems unlikely that the phenomena described here are solely artifacts of PSD-95:GFP overexpression, although, as mentioned above, we cannot exclude the possibility of the introduction of some quantitative inaccuracies [79]. | |||||||||||||||
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A potential concern is the use of an exogenous form of PSD-95 fused to EGFP (an ? | |||||||||||||||
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These experiments strongly indicate that the characteristics of network activity recorded through the MEA faithfully represent, at least to a first approximation, the activity of PSD-95:GFP-expressing neurons. | |||||||||||||||
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Collectively, the study demonstrates that maternal exposure to morphine decreases the magnitude of PSD-95, nNOS, the phosphorylation of CREB(Serine-133), and LTD expression in hippocampal CA1 subregion of young offspring (e.g., P14). | |||||||||||||||
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Furthermore, the tight correlation between network bursts and calcium transients suggests that these neurons respond well to excitatory synapse activation, implying that PSD-95:GFP expression does not severely impair glutamatergic synapse functionality. | |||||||||||||||
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This remodeling is not an artifact of imaging-related noise because practically no change in M was observed in control experiments performed in exactly the same experimental conditions using paraformaldehyde-fixed, PSD-95:GFP-expressing neurons (Figure 8A and 8B). | |||||||||||||||
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For example, in the first study mentioned above, PSD-95:GFP expression levels were reported to be many fold greater than endogenous PSD-95 levels, whereas the use of Sindbis or Semliki forest viral vectors for PSD-95:GFP expression in others might have resulted in similar situations [72]. | |||||||||||||||
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The rats injected intrathecally with postsynaptic density protein-95 antisense oligodeoxynucleotide every 24 h for 4 days from day 7 to day 10 post-surgery exhibited not only a marked decrease in spinal cord postsynaptic density protein-95 protein expression but also a significant reduction in mechanical and thermal hyperalgesia on day 11 post-surgery. | |||||||||||||||
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