INT93308

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Context Info
Confidence 0.09
First Reported 2001
Last Reported 2008
Negated 0
Speculated 0
Reported most in Body
Documents 13
Total Number 18
Disease Relevance 2.00
Pain Relevance 2.92

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
SH-SY5Y 2
central nervous system 2
sensory neurons 1
neurons 1
uterine 1
PIRT (Homo sapiens)
PIRT - I136V (3)
Pain Link Frequency Relevance Heat
qutenza 16 100.00 Very High Very High Very High
Calcium channel 10 100.00 Very High Very High Very High
ASIC 6 100.00 Very High Very High Very High
Central nervous system 3 98.88 Very High Very High Very High
Dismenorea 10 96.60 Very High Very High Very High
Analgesic 4 89.24 High High
Pain 93 88.88 High High
tissue acidosis 3 88.80 High High
sodium channel 13 86.80 High High
addiction 42 83.08 Quite High
Disease Link Frequency Relevance Heat
Dysmenorrhea 10 96.60 Very High Very High Very High
Pain 93 88.88 High High
Acidosis 3 88.80 High High
Erythermalgia 150 87.36 High High
Tinnitus 2 80.32 Quite High
Ganglion Cysts 7 75.00 Quite High
Congenital Pain Insensitivity 6 25.40 Quite Low
Channelopathies 6 23.68 Low Low
Somatoform Disorder 12 20.32 Low Low
Non-diabetic Peripheral Neuropathy 6 18.96 Low Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
METHODS: Patch-clamp recordings were performed on the pharmacologic action of lidocaine on Kv 3.1 channels natively expressed in SH-SY5Y cells and Kv 1.1 channels expressed in HEK 293 cells.
Gene_expression (expressed) of channels in SH-SY5Y
1) Confidence 0.09 Published 2006 Journal Otol. Neurotol. Section Body Doc Link 16371858 Disease Relevance 0.08 Pain Relevance 0
METHODS: Patch-clamp recordings were performed on the pharmacologic action of lidocaine on Kv 3.1 channels natively expressed in SH-SY5Y cells and Kv 1.1 channels expressed in HEK 293 cells.
Gene_expression (expressed) of channels in SH-SY5Y
2) Confidence 0.09 Published 2006 Journal Otol. Neurotol. Section Body Doc Link 16371858 Disease Relevance 0.08 Pain Relevance 0
Eugenol inhibited calcium currents in the E52 cell line, stably expressing the human Ca(V)2.3 calcium channels, where TRPV1 is not endogenously expressed.
Gene_expression (expressing) of channels associated with calcium channel
3) Confidence 0.04 Published 2008 Journal J. Dent. Res. Section Abstract Doc Link 18218839 Disease Relevance 0 Pain Relevance 0.67
Here, using whole-cell patch-clamp electrophysiology and reverse transcriptase-polymerase chain reaction (RT-PCR), we show that HEK293 cells, a commonly used cell line for the expression and characterisation of many ion channels, functionally express an endogenous proton-gated conductance attributable to the activity of human ASIC1a.
Gene_expression (expression) of channels
4) Confidence 0.03 Published 2001 Journal Pflugers Arch. Section Abstract Doc Link 11512022 Disease Relevance 0.18 Pain Relevance 0.25
Acid-sensing ion channels (ASICs) are a new and expanding family of proton-gated cation (Na+/Ca2+) channels that are widely expressed in sensory neurons and the central nervous system.
Gene_expression (expressed) of channels in central nervous system associated with central nervous system and asic
5) Confidence 0.03 Published 2001 Journal Pflugers Arch. Section Abstract Doc Link 11512022 Disease Relevance 0.18 Pain Relevance 0.23
Acid-sensing ion channels (ASICs) are a new and expanding family of proton-gated cation (Na+/Ca2+) channels that are widely expressed in sensory neurons and the central nervous system.
Gene_expression (expressed) of channels in central nervous system associated with central nervous system and asic
6) Confidence 0.03 Published 2001 Journal Pflugers Arch. Section Abstract Doc Link 11512022 Disease Relevance 0.18 Pain Relevance 0.23
However, the regulatory mechanism(s) governing the expression of Maxi-K channels with decreased calcium sensitivity at parturition are unclear.
Gene_expression (expression) of channels
7) Confidence 0.01 Published 2004 Journal Reprod Biol Endocrinol Section Abstract Doc Link PMC524189 Disease Relevance 0.10 Pain Relevance 0.10
Our findings suggest that decreased Maxi-K alpha subunit mRNA expression in human myometrium at labour onset, coupled to an increased proportion of Maxi-K channels expressing the 132 bp spliced exon, may be linked to decreased Maxi-K channel calcium and voltage sensitivity, thereby promoting enhanced uterine activity at the time of labour.



Gene_expression (expressing) of channels in uterine
8) Confidence 0.01 Published 2004 Journal Reprod Biol Endocrinol Section Abstract Doc Link PMC524189 Disease Relevance 0 Pain Relevance 0
subunits expressing this variant was very low, accounting for only ~1% of Maxi-K channels expressed in the three tissues sets.
Gene_expression (expressed) of channels
9) Confidence 0.01 Published 2004 Journal Reprod Biol Endocrinol Section Body Doc Link PMC524189 Disease Relevance 0 Pain Relevance 0
An increased proportion of 132 bp exon-containing alpha subunit variants with labour onset is of interest, as channels expressing this spliced exon have decreased calcium and voltage sensitivities.


Gene_expression (expressing) of channels
10) Confidence 0.01 Published 2004 Journal Reprod Biol Endocrinol Section Abstract Doc Link PMC524189 Disease Relevance 0 Pain Relevance 0
However, the proportion of Maxi-K channels expressing the 132 bp spliced exon was significantly increased with labour onset (PL), compared to both non-pregnant (NP)(P < 0.05) and pregnant not-in-labour myometrial tissues (PNL) (P < 0.01)(Figure 3B).
Gene_expression (expressing) of channels
11) Confidence 0.01 Published 2004 Journal Reprod Biol Endocrinol Section Body Doc Link PMC524189 Disease Relevance 0 Pain Relevance 0
Heterologously expressed vanilloid receptor 1 (VR1), a cloned cDNA encoding for capsaicin (CAP)-sensitive currents, resembles the native CAP channels in cultured sensory neurons in channel property.
Gene_expression (expressed) of channels in sensory neurons associated with qutenza
12) Confidence 0.01 Published 2001 Journal Neurosci. Lett. Section Abstract Doc Link 11166956 Disease Relevance 0.07 Pain Relevance 0.77
Human embryonic kidney cells (HEK293) stably expressing either wild-type (NaV1.7R) or mutant (I136V) channels were generated as described before [21].


Gene_expression (expressing) of channels in embryonic kidney
13) Confidence 0.01 Published 2008 Journal Mol Pain Section Body Doc Link PMC2262064 Disease Relevance 0.42 Pain Relevance 0.22
Sodium currents were recorded from HEK293 cells stably expressing either wild type NaV1.7R or I136V mutant channels.
Gene_expression (expressing) of channels (I136V)
14) Confidence 0.01 Published 2008 Journal Mol Pain Section Body Doc Link PMC2262064 Disease Relevance 0.17 Pain Relevance 0.17
HEK293 cells expressing mutant channels produced larger ramp currents than cells expressing wild type channels (I136V: 0.79 ± 0.04%, n = 26; WT: 0.23 ± 0.02%, n = 19, p < 0.05).
Gene_expression (expressing) of channels (I136V)
15) Confidence 0.01 Published 2008 Journal Mol Pain Section Body Doc Link PMC2262064 Disease Relevance 0.07 Pain Relevance 0.03
Whole-cell voltage-clamp recordings of HEK293 cells expressing either wild type NaV1.7R or I136V mutant channels were performed at room temperature (20–22°C) using an Axopatch 200 B amplifier (Axon Instruments, Foster City, CA).
Gene_expression (expressing) of channels (I136V)
16) Confidence 0.01 Published 2008 Journal Mol Pain Section Body Doc Link PMC2262064 Disease Relevance 0.34 Pain Relevance 0.20
I136V mutant channels are predicted to produce larger window currents than wild type channels, and thus are expected to lead to a more depolarized resting membrane potential in DRG neurons [20,23].


Gene_expression (produce) of channels in neurons
17) Confidence 0.01 Published 2008 Journal Mol Pain Section Body Doc Link PMC2262064 Disease Relevance 0.08 Pain Relevance 0.03
HEK293 cells expressing mutant channels produced larger ramp currents than cells expressing wild type channels (I136V: 0.79 ± 0.04%, n = 26; WT: 0.23 ± 0.02%, n = 19, p < 0.05).
Gene_expression (expressing) of channels
18) Confidence 0.01 Published 2008 Journal Mol Pain Section Body Doc Link PMC2262064 Disease Relevance 0.06 Pain Relevance 0.03

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