INT94303

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Context Info
Confidence 0.04
First Reported 2000
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 44
Total Number 46
Disease Relevance 11.62
Pain Relevance 3.87

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

RNA binding (Upf1) helicase activity (Upf1) cell cycle (Upf1)
DNA binding (Upf1) cytoplasm (Upf1)
Anatomy Link Frequency
brain 3
T cells 3
skeletal muscle 2
cleavage 2
embryos 1
Upf1 (Mus musculus)
Pain Link Frequency Relevance Heat
Delta opioid receptors 10 100.00 Very High Very High Very High
GABAergic 675 99.64 Very High Very High Very High
Pyramidal cell 88 99.24 Very High Very High Very High
Central nervous system 71 98.44 Very High Very High Very High
cytokine 38 97.80 Very High Very High Very High
opiate 4 96.72 Very High Very High Very High
gABA 173 96.40 Very High Very High Very High
metalloproteinase 65 94.56 High High
medulla 28 93.12 High High
Calcium channel 88 92.68 High High
Disease Link Frequency Relevance Heat
Targeted Disruption 478 99.76 Very High Very High Very High
Frailty 379 99.64 Very High Very High Very High
Disease 223 99.24 Very High Very High Very High
Sprains And Strains 131 99.04 Very High Very High Very High
Injury 115 98.00 Very High Very High Very High
Spinocerebellar Ataxia Type 2 90 97.28 Very High Very High Very High
Cytomegalovirus Infection 44 96.88 Very High Very High Very High
Neuroblastoma 22 96.20 Very High Very High Very High
Movement Disorders 36 95.76 Very High Very High Very High
Huntington's Chorea 36 95.60 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The critical distinction between the two genes occurs at the RNA processing level: SMN1 produces full-length transcripts, while SMN2 primarily produces an alternatively spliced transcript lacking the final coding exon [10].
Gene_expression (produces) of transcripts
1) Confidence 0.04 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2565107 Disease Relevance 1.14 Pain Relevance 0.03
Recently, quiescent T cells were found to express low levels of DOR transcripts, which increased due to the following: cell culture of unstimulated murine splenocytes (depending on cell density); cross-linking the T-cell receptor (TCR) with anti-CD3-epsilon; and a single in vivo exposure to staphylococcal enterotoxin B (SEB).
Gene_expression (express) of transcripts in T cells
2) Confidence 0.04 Published 2000 Journal Ann. N. Y. Acad. Sci. Section Abstract Doc Link 11268405 Disease Relevance 0 Pain Relevance 0.30
Since expression patterns of these transcripts correlated to the chromosome 12 locus, it follows that their expression patterns are correlated to each other: Fos, Nr4a1, and Id2 were positively co-regulated by C57BL/6 alleles and were also positively correlated with each other (r > 0.68), whereas they were negatively correlated with Gfap (r < ?
Gene_expression (expression) of transcripts
3) Confidence 0.03 Published 2007 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2759132 Disease Relevance 0.37 Pain Relevance 0
Since these transcripts were also differentially expressed in injured retina, this network may also control wound-healing events in the retina and elsewhere in the CNS.


Gene_expression (expressed) of transcripts associated with injury and central nervous system
4) Confidence 0.03 Published 2007 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2759132 Disease Relevance 0.29 Pain Relevance 0.05
Expression of delta opioid receptors and transcripts by splenic T cells.
Gene_expression (Expression) of transcripts in T cells associated with delta opioid receptors
5) Confidence 0.03 Published 2000 Journal Ann. N. Y. Acad. Sci. Section Title Doc Link 11268405 Disease Relevance 0 Pain Relevance 0.38
Our results, summarized in Table 1, demonstrate that a relatively high level of VGAT transcripts was detected in the absence of a VGLUT1 signal in GFPpos samples, and conversely, a similarly high level of expression of VGLUT1 was determined for GFPneg cells in which VGAT RNA was undetectable.
Gene_expression (detected) of transcripts
6) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0.08 Pain Relevance 0.13
As shown in Fig. 6C, qRT-RCR was readily able to distinguish a 7.5-fold and 5-fold greater expression of SNAP-25a transcripts in young P24 (post-weaning) and adult (P124) knockin mice, respectively, relative to wild type, which is comparable to level of overexpression of SNAP-25a transcripts previously determined for these mice based on isoform-specific cleavage by restriction endonucleases of total SNAP-25 PCR amplified cDNA [see ref. [22]].
Gene_expression (expression) of transcripts in cleavage
7) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
Synaptogenesis and maturation of functional synaptic connectivity is accompanied by dramatically increased levels of SNAP-25 [14], as well as a significant change in the relative expression of transcripts encoding two isoforms of the protein that are produced by alternative splicing between divergent, tandem arranged copies of a single exon [15].
Gene_expression (produced) of transcripts
8) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.09
Synaptogenesis and maturation of functional synaptic connectivity is accompanied by dramatically increased levels of SNAP-25 [14], as well as a significant change in the relative expression of transcripts encoding two isoforms of the protein that are produced by alternative splicing between divergent, tandem arranged copies of a single exon [15].
Gene_expression (expression) of transcripts
9) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.09
Using a more sensitive, quantitative qRT-PCR assay, we show here that the expression of SNAP-25a and 25b RNA transcripts compared to ?
Gene_expression (expression) of transcripts
10) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.11
Taken together, these results demonstrated that the RT-PCR assay with these primers provided a quantitative measure of the relative expression of transcripts encoding the two SNAP-25 isoforms.
Gene_expression (expression) of transcripts
11) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
Furthermore, we observed that although the expression of SNAP-25 RNA transcripts varied among neuronal populations in adult brain, the relative ratio of the transcripts encoding alternatively spliced SNAP-25 variant isoforms was not different in GABAergic and glutamatergic neurons.


Gene_expression (expression) of transcripts in adult brain associated with gabaergic
12) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Abstract Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0.49
Analysis of cellular transcripts by qRT-PCR revealed no significant difference in the relative levels of SNAP-25a and SNAP-25b transcripts between GABAergic projection neurons and interneurons, regardless of anatomical location, and nonGABAergic (glutaminergic) hippocampal pyramidal and cerebellar granule cells (p = 0.25, one-way ANOVA, Tukey's Multiple Comparison Test, Fig. 5B).
Gene_expression (levels) of transcripts in cerebellar granule cells associated with pyramidal cell and gabaergic
13) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0.06 Pain Relevance 0.38
To assess the ability to quantify SNAP-25 isoforms from brain tissue, we also compared amplification from cDNAs prepared from cortex of Snap25+/+ and a Snap25 knockin mutant (Snap25tkneo/tkneo) that overexpresses SNAP-25a transcripts [22].
Gene_expression (overexpresses) of transcripts in cortex
14) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
As shown in Fig. 6C, qRT-RCR was readily able to distinguish a 7.5-fold and 5-fold greater expression of SNAP-25a transcripts in young P24 (post-weaning) and adult (P124) knockin mice, respectively, relative to wild type, which is comparable to level of overexpression of SNAP-25a transcripts previously determined for these mice based on isoform-specific cleavage by restriction endonucleases of total SNAP-25 PCR amplified cDNA [see ref. [22]].
Gene_expression (overexpression) of transcripts in cleavage
15) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0 Pain Relevance 0
Preliminary experiments established that the primer sets were equally efficient in amplifying the specific isoform sequences from cDNA templates, and RNA transcripts prepared from brains of SNAP-25a overexpressing mutant and control mice, as well as from cells transfected with cDNAs encoding the individual isoforms (see Methods), thereby validating the RT-PCR assay as a measure of the relative expression of the two isoform transcripts.
Gene_expression (expression) of transcripts in brains
16) Confidence 0.02 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2600647 Disease Relevance 0.12 Pain Relevance 0.20
The oligonucleotide-directed production of small RNA transcripts with T7 RNA polymerase were made with Ampliscribe™ T7 transcription kit (Epicentre Biotechnologies; Madison WI, USA) according to manufacturer's instructions.


Gene_expression (production) of transcripts
17) Confidence 0.02 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794538 Disease Relevance 0 Pain Relevance 0.04
Interestingly, some cells in the subventricular layer expressed proteolipid protein transcripts, and 3 h after a H--I insult they were degenerating in the injured but not contralateral side.
Gene_expression (expressed) of transcripts
18) Confidence 0.02 Published 2001 Journal Int. J. Dev. Neurosci. Section Abstract Doc Link 11255033 Disease Relevance 0.71 Pain Relevance 0.05
The levels of transcripts were very similar among all constructs, indicating that the point mutations did not alter transcription levels.
Gene_expression (levels) of transcripts
19) Confidence 0.02 Published 2007 Journal Nucleic Acids Research Section Body Doc Link PMC1865057 Disease Relevance 0.20 Pain Relevance 0.26
The levels of transcripts were very similar among all constructs, indicating that these mutations did not alter transcription levels (Figure 6B, lower panel).
Gene_expression (levels) of transcripts
20) Confidence 0.02 Published 2007 Journal Nucleic Acids Research Section Body Doc Link PMC1865057 Disease Relevance 0 Pain Relevance 0.03

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