INT94356
From wiki-pain
|
|
|
|
|
Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
Interestingly, a recent thermodynamic analysis of Cl- transport in DRG neurons indicated that the regulation of NKCC1 activity by phosphorylation/dephosphorylation may account for substantial changes in ECl [3]. | |||||||||||||||
| |||||||||||||||
|
We investigated changes in NKCC1 protein levels in the epicenter of spinal cord tissue harvested at 2, 7, and 14 days post-SCI. | |||||||||||||||
| |||||||||||||||
|
The activity of NKCC1 and KCC2 can be modulated by phosphorylation [26,27]. | |||||||||||||||
| |||||||||||||||
|
These data demonstrate that NKCC1 and KCC2 expression levels remain unchanged over 2 hr of treatment with inflammatory mediators. | |||||||||||||||
| |||||||||||||||
|
To further establish that changes in NKCC1 and KCC2 are specific to the lesion epicenter of the spinal cord in response to SCI, we also examined spinal cord tissue rostral to the SCI injury epicenter. | |||||||||||||||
| |||||||||||||||
|
On the other hand, intraplantar formalin stimulation triggers a significant decrease in KCC2 protein expression without changes in NKCC1 in the rat spinal cord [18]. | |||||||||||||||
| |||||||||||||||
|
The significance of changes of NKCC1 and KCC2 proteins following SCI is not clear. | |||||||||||||||
| |||||||||||||||
|
We examined changes in NKCC1 and KCC2 protein levels in the contusion epicenter of spinal cord during days 2142 post-SCI, when it was possible to determine the presence of chronic TH. | |||||||||||||||
| |||||||||||||||
|
Immunoblotting showed a significant transient up-regulation of NKCC1 protein in the lesion epicenter of the rat spinal cord on day 14 post-SCI. | |||||||||||||||
| |||||||||||||||
|
However, in contrast to the NKCC1 immunosignal, the p-NKCC1 signal responded rapidly and robustly to the inflammatory mediators. | |||||||||||||||
| |||||||||||||||
|
Immunohistochemistry of NKCC1 and KCC2, the main neuronal Cl- importer and exporter, respectively, exposed an inverse regulation by the inflammatory mediators. | |||||||||||||||
| |||||||||||||||
|
The present work was aimed at examining the possible involvement of different parts of the septal area (dorsal, medial, lateral, and septohypothalamic nucleus), the basolateral amygdala, and the bed nucleus of the stria terminalis (BNST) in the regulation of the cytotoxic activity of NK cells (NKCC). | |||||||||||||||
| |||||||||||||||
|
In summary, we investigated the roles of two major intracellular Cl- regulatory proteins, NKCC1 and KCC2, in chronic neuropathic pain following contusive SCI in the rat model. | |||||||||||||||
| |||||||||||||||
|
We, therefore, investigated expression changes of KCC2 and NKCC1 in the spinal dorsal horn of the rat after the intraplantar injection of formalin as an acute nociceptive stimulus. | |||||||||||||||
| |||||||||||||||
|
We have recently described the up-regulation of the CSF-secreting cation chloride co-transporter NKCC1 in AC membranes compared with normal arachnoid [8]. | |||||||||||||||
| |||||||||||||||
|
General Comments
This test has worked.