INT94356

Context Info Confidence 0.58 First Reported 2001 Last Reported 2010 Negated 2 Speculated 4 Reported most in Body Documents 11 Total Number 15 Disease Relevance 12.80 Pain Relevance 6.59

plasma membrane (Slc12a2)

Anatomy Link Frequency spinal cord 5 neuronal 1 spinal 1 neurons 1 amygdala 1 Slc12a2 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Inflammatory mediators 230 99.68 spinal dorsal horn 8 99.68 Spinal cord 196 99.52 Neuropathic pain 132 99.12 amygdala 2 98.80 Hyperalgesia 160 98.72 Acute pain 1 98.12 GABAergic 81 96.80 gABA 103 93.92 Thermal hyperalgesia 140 93.88

Disease Link Frequency Relevance Heat Arachnoid Cysts 44 99.84 INFLAMMATION 338 99.68 Spinal Cord Injury 504 99.48 Neuropathic Pain 208 99.12 Hyperalgesia 356 98.72 Contusions 28 98.72 Pain 48 98.12 Cyst 40 97.28 Nociception 110 96.20 Injury 85 95.24

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Interestingly, a recent thermodynamic analysis of Cl- transport in DRG neurons indicated that the regulation of NKCC1 activity by phosphorylation/dephosphorylation may account for substantial changes in ECl [3].

Regulation (regulation) of NKCC1 in neurons

1) Confidence 0.58 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.31 Pain Relevance 0.34

We investigated changes in NKCC1 protein levels in the epicenter of spinal cord tissue harvested at 2, 7, and 14 days post-SCI.

Spec (investigated) Regulation (changes) of NKCC1 in spinal cord associated with spinal cord injury and spinal cord

2) Confidence 0.52 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 1.08 Pain Relevance 0.42

The activity of NKCC1 and KCC2 can be modulated by phosphorylation [26,27].

Regulation (modulated) of NKCC1

3) Confidence 0.42 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.17 Pain Relevance 0.09

These data demonstrate that NKCC1 and KCC2 expression levels remain unchanged over 2 hr of treatment with inflammatory mediators.

Neg (unchanged) Regulation (unchanged) of NKCC1 associated with inflammatory mediators

4) Confidence 0.42 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.17 Pain Relevance 0.08

To further establish that changes in NKCC1 and KCC2 are specific to the lesion epicenter of the spinal cord in response to SCI, we also examined spinal cord tissue rostral to the SCI injury epicenter.

Regulation (specific) of NKCC1 in spinal cord associated with spinal cord injury, injury and spinal cord

5) Confidence 0.38 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 1.57 Pain Relevance 0.35

On the other hand, intraplantar formalin stimulation triggers a significant decrease in KCC2 protein expression without changes in NKCC1 in the rat spinal cord [18].

Neg (without) Regulation (changes) of NKCC1 in spinal cord associated with spinal cord

6) Confidence 0.38 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 1.23 Pain Relevance 1.13

The significance of changes of NKCC1 and KCC2 proteins following SCI is not clear.

Regulation (changes) of NKCC1 associated with spinal cord injury

7) Confidence 0.38 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 0.79 Pain Relevance 0.34

We examined changes in NKCC1 and KCC2 protein levels in the contusion epicenter of spinal cord during days 21–42 post-SCI, when it was possible to determine the presence of chronic TH.

Spec (examined) Regulation (changes) of NKCC1 in spinal cord associated with spinal cord injury, thermal hyperalgesia, contusions and spinal cord

8) Confidence 0.38 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 1.87 Pain Relevance 0.56

Immunoblotting showed a significant transient up-regulation of NKCC1 protein in the lesion epicenter of the rat spinal cord on day 14 post-SCI.

Regulation (regulation) of NKCC1 in spinal cord associated with spinal cord injury and spinal cord

9) Confidence 0.38 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 1.24 Pain Relevance 1.17

However, in contrast to the NKCC1 immunosignal, the p-NKCC1 signal responded rapidly and robustly to the inflammatory mediators.

Regulation (responded) of p-NKCC1 associated with inflammatory mediators

10) Confidence 0.37 Published 2008 Journal Mol Pain Section Body Doc Link PMC2526990 Disease Relevance 0.36 Pain Relevance 0.18

Immunohistochemistry of NKCC1 and KCC2, the main neuronal Cl- importer and exporter, respectively, exposed an inverse regulation by the inflammatory mediators.

Regulation (regulation) of NKCC1 in neuronal associated with inflammatory mediators

11) Confidence 0.25 Published 2008 Journal Mol Pain Section Abstract Doc Link PMC2526990 Disease Relevance 0.73 Pain Relevance 0.44

The present work was aimed at examining the possible involvement of different parts of the septal area (dorsal, medial, lateral, and septohypothalamic nucleus), the basolateral amygdala, and the bed nucleus of the stria terminalis (BNST) in the regulation of the cytotoxic activity of NK cells (NKCC).

Regulation (regulation) of NKCC in amygdala associated with amygdala

12) Confidence 0.24 Published 2001 Journal Brain Behav. Immun. Section Abstract Doc Link 11259084 Disease Relevance 0.06 Pain Relevance 0.14

In summary, we investigated the roles of two major intracellular Cl- regulatory proteins, NKCC1 and KCC2, in chronic neuropathic pain following contusive SCI in the rat model.

Spec (investigated) Regulation (roles) of NKCC1 associated with spinal cord injury and neuropathic pain

13) Confidence 0.23 Published 2008 Journal Mol Pain Section Body Doc Link PMC2561007 Disease Relevance 1.13 Pain Relevance 0.63

We, therefore, investigated expression changes of KCC2 and NKCC1 in the spinal dorsal horn of the rat after the intraplantar injection of formalin as an acute nociceptive stimulus.

Spec (investigated) Regulation (changes) of NKCC1 in spinal associated with nociception and spinal dorsal horn

14) Confidence 0.12 Published 2006 Journal Neurosci. Res. Section Abstract Doc Link 17007947 Disease Relevance 0.65 Pain Relevance 0.70

We have recently described the up-regulation of the CSF-secreting cation chloride co-transporter NKCC1 in AC membranes compared with normal arachnoid [8].

Regulation (regulation) of NKCC1 associated with arachnoid cysts

15) Confidence 0.09 Published 2010 Journal Cerebrospinal Fluid Res Section Body Doc Link PMC2898803 Disease Relevance 1.43 Pain Relevance 0

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