INT95099
From wiki-pain
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Sentences Mentioned In
Key: | Protein | Mutation | Event | Anatomy | Negation | Speculation | Pain term | Disease term |
The purpose of this study was to evaluate NSAIDs that compete less with methotrexate by using the renal cell line stably expressing human OAT3 (S2-hOAT3) in vitro. | |||||||||||||||
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Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. | |||||||||||||||
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The purpose of this study was to evaluate NSAIDs that compete less with methotrexate by using the renal cell line stably expressing human OAT3 (S2-hOAT3) in vitro. | |||||||||||||||
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Evaluation of the interaction between nonsteroidal anti-inflammatory drugs and methotrexate using human organic anion transporter 3-transfected cells. | |||||||||||||||
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When expressed in Xenopus laevis oocytes, hOAT3 mediated the transport of estrone sulfate (K(m) = 3.1 microM), p-aminohippurate (K(m) = 87.2 microM), methotrexate (K(m) = 10.9 microM), and cimetidine (K(m) = 57.4 microM) in a sodium-independent manner. hOAT3 also mediated the transport of dehydroepiandrosterone sulfate, ochratoxin A, PGE(2), estradiol glucuronide, taurocholate, glutarate, cAMP and uric acid. | |||||||||||||||
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MRP1, MRP2, MRP5, OAT3, and OAT4 were also detected by RT-PCR. | |||||||||||||||
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Mouse proximal tubule cells stably expressing basolateral human organic anion transporters (hOAT1 and hOAT3) and apical hOAT (hOAT4) were established. | |||||||||||||||
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Of the 15 genes nearest AR occupied regions, expression of all but SLC22A8 was detectable, and only 6 of the remaining 14 genes responded to DHT treatment in a consistent manner. | |||||||||||||||
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For example, the active efflux transporters P-glycoprotein (Abcb1, Pgp) and breast cancer resistance protein (Abcg2, Bcrp) are located in the luminal membrane (25,26), while the organic anion transporter 3 (SLC22A8, Oat3) has been found in the abluminal membrane (27), indicating differences in their function. | |||||||||||||||
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Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. | |||||||||||||||
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Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle. | |||||||||||||||
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Furthermore, organic anion transporter 3 (OAT3) is expressed at the BBB and mediates the efflux transport of homovanillic acid, a dopamine metabolite. | |||||||||||||||
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Induction of AKI in ischemia-reperfusion rat models demonstrates decreased OAT1 and OAT3 mRNA as well as protein expression [34-36]. | |||||||||||||||
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For example, the active efflux transporters P-glycoprotein (Abcb1, Pgp) and breast cancer resistance protein (Abcg2, Bcrp) are located in the luminal membrane (25,26), while the organic anion transporter 3 (SLC22A8, Oat3) has been found in the abluminal membrane (27), indicating differences in their function. | |||||||||||||||
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General Comments
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