INT95099

Context Info Confidence 0.67 First Reported 2001 Last Reported 2008 Negated 0 Speculated 0 Reported most in Abstract Documents 14 Total Number 14 Disease Relevance 1.59 Pain Relevance 5.14

transmembrane transporter activity (SLC22A8) plasma membrane (SLC22A8) transmembrane transport (SLC22A8)

Anatomy Link Frequency proximal 1 brain 1 skeletal muscle 1 oocytes 1 kidney 1 SLC22A8 (Homo sapiens)

Pain Link Frequency Relevance Heat methotrexate 59 99.74 cINOD 48 99.40 ischemia 1 98.88 Dopamine 1 97.20 Morphine 44 93.28 dexamethasone 1 91.68 Opioid 13 87.20 aspirin 4 82.80 vincristine 1 82.08 Paracetamol 6 79.92

Disease Link Frequency Relevance Heat INFLAMMATION 14 99.28 Cv Unclassified Under Development 1 98.88 Injury 92 98.64 Breast Cancer 2 97.64 Prostate Cancer 83 82.56 Cancer 25 73.20 Disease 11 42.00 Chronic Renal Failure 8 5.00 Repression 7 5.00 Critical Illness 7 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The purpose of this study was to evaluate NSAIDs that compete less with methotrexate by using the renal cell line stably expressing human OAT3 (S2-hOAT3) in vitro.

Gene_expression (expressing) of OAT3 associated with cinod and methotrexate

1) Confidence 0.67 Published 2008 Journal Eur. J. Pharmacol. Section Abstract Doc Link 18789319 Disease Relevance 0.16 Pain Relevance 1.20

Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle.

Gene_expression (expressed) of hOAT3 in brain

2) Confidence 0.57 Published 2001 Journal Mol. Pharmacol. Section Abstract Doc Link 11306713 Disease Relevance 0 Pain Relevance 0.04

The purpose of this study was to evaluate NSAIDs that compete less with methotrexate by using the renal cell line stably expressing human OAT3 (S2-hOAT3) in vitro.

Gene_expression (expressing) of hOAT3 associated with cinod and methotrexate

3) Confidence 0.52 Published 2008 Journal Eur. J. Pharmacol. Section Abstract Doc Link 18789319 Disease Relevance 0.16 Pain Relevance 1.21

Evaluation of the interaction between nonsteroidal anti-inflammatory drugs and methotrexate using human organic anion transporter 3-transfected cells.

Gene_expression (transfected) of organic anion transporter 3 associated with inflammation, cinod and methotrexate

4) Confidence 0.52 Published 2008 Journal Eur. J. Pharmacol. Section Title Doc Link 18789319 Disease Relevance 0.20 Pain Relevance 1.42

When expressed in Xenopus laevis oocytes, hOAT3 mediated the transport of estrone sulfate (K(m) = 3.1 microM), p-aminohippurate (K(m) = 87.2 microM), methotrexate (K(m) = 10.9 microM), and cimetidine (K(m) = 57.4 microM) in a sodium-independent manner. hOAT3 also mediated the transport of dehydroepiandrosterone sulfate, ochratoxin A, PGE(2), estradiol glucuronide, taurocholate, glutarate, cAMP and uric acid.

Gene_expression (expressed) of hOAT3 in oocytes associated with methotrexate

5) Confidence 0.51 Published 2001 Journal Mol. Pharmacol. Section Abstract Doc Link 11306713 Disease Relevance 0.05 Pain Relevance 0.10

MRP1, MRP2, MRP5, OAT3, and OAT4 were also detected by RT-PCR.

Gene_expression (detected) of OAT3

6) Confidence 0.48 Published 2005 Journal Brain Res. Section Abstract Doc Link 16289483 Disease Relevance 0 Pain Relevance 0.14

Mouse proximal tubule cells stably expressing basolateral human organic anion transporters (hOAT1 and hOAT3) and apical hOAT (hOAT4) were established.

Gene_expression (expressing) of hOAT3 in proximal

7) Confidence 0.44 Published 2002 Journal J. Pharmacol. Exp. Ther. Section Abstract Doc Link 12130730 Disease Relevance 0.09 Pain Relevance 0.51

Of the 15 genes nearest AR occupied regions, expression of all but SLC22A8 was detectable, and only 6 of the remaining 14 genes responded to DHT treatment in a consistent manner.

Gene_expression (expression) of SLC22A8

8) Confidence 0.36 Published 2007 Journal Mol Cancer Section Body Doc Link PMC1904239 Disease Relevance 0.16 Pain Relevance 0.03

For example, the active efflux transporters P-glycoprotein (Abcb1, Pgp) and breast cancer resistance protein (Abcg2, Bcrp) are located in the luminal membrane (25,26), while the organic anion transporter 3 (SLC22A8, Oat3) has been found in the abluminal membrane (27), indicating differences in their function.

Gene_expression (found) of SLC22A8 associated with breast cancer

9) Confidence 0.21 Published 2007 Journal Pharm Res Section Body Doc Link PMC2469271 Disease Relevance 0.10 Pain Relevance 0

Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle.

Gene_expression (expressed) of hOAT3 in skeletal muscle

10) Confidence 0.19 Published 2001 Journal Mol. Pharmacol. Section Abstract Doc Link 11306713 Disease Relevance 0 Pain Relevance 0.04

Northern blot analysis revealed that hOAT3 mRNA is expressed in the kidney, brain, and skeletal muscle.

Gene_expression (expressed) of hOAT3 in kidney

11) Confidence 0.19 Published 2001 Journal Mol. Pharmacol. Section Abstract Doc Link 11306713 Disease Relevance 0 Pain Relevance 0.04

Furthermore, organic anion transporter 3 (OAT3) is expressed at the BBB and mediates the efflux transport of homovanillic acid, a dopamine metabolite.

Gene_expression (expressed) of OAT3 associated with dopamine

12) Confidence 0.15 Published 2003 Journal Nippon Yakurigaku Zasshi Section Abstract Doc Link 12843573 Disease Relevance 0 Pain Relevance 0.14

Induction of AKI in ischemia-reperfusion rat models demonstrates decreased OAT1 and OAT3 mRNA as well as protein expression [34-36].

Gene_expression (expression) of OAT3 associated with ischemia and injury

13) Confidence 0.10 Published 2008 Journal Crit Care Section Body Doc Link PMC2646335 Disease Relevance 0.57 Pain Relevance 0.26

For example, the active efflux transporters P-glycoprotein (Abcb1, Pgp) and breast cancer resistance protein (Abcg2, Bcrp) are located in the luminal membrane (25,26), while the organic anion transporter 3 (SLC22A8, Oat3) has been found in the abluminal membrane (27), indicating differences in their function.

Gene_expression (found) of Oat3 associated with breast cancer

14) Confidence 0.09 Published 2007 Journal Pharm Res Section Body Doc Link PMC2469271 Disease Relevance 0.10 Pain Relevance 0

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