INT96928

Context Info Confidence 0.03 First Reported 2001 Last Reported 2001 Negated 0 Speculated 0 Reported most in Abstract Documents 1 Total Number 1 Disease Relevance 0.09 Pain Relevance 0.09

cytosol (Alox5) nuclear envelope (Alox5) oxidoreductase activity (Alox5) nucleus (Alox5) cytoplasm (Alox5)

Alox5 (Rattus norvegicus)

Pain Link Frequency Relevance Heat cINOD 1 86.48

Disease Link Frequency Relevance Heat INFLAMMATION 1 86.24

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In our attempt to characterize the stimulating constituents, we identified the tetracyclic triterpene 3-oxo-tirucallic acid (3-oxo-TA), which, in the range from 2.5 to 15 microM, enhanced 5-LO product formation in ionophore-challenged polymorphonuclear cells (PMNs) (e.g., from 1981 +/- 177 to 3042 +/- 208 pmol at 10 microM 3-oxo-TA), and initiated Ca(2+) mobilization, MEK-1/2 phosphorylation, 5-LO translocation, and 5-LO product formation in resting cells (534 +/- 394 pmol/5 x 10(6) PMNs).

Positive_regulation (enhanced) of 5-LO Binding (formation) of

1) Confidence 0.03 Published 2001 Journal Mol. Pharmacol. Section Abstract Doc Link 11455013 Disease Relevance 0.09 Pain Relevance 0.09

General Comments

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