INT97450

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Context Info
Confidence 0.75
First Reported 2001
Last Reported 2010
Negated 1
Speculated 0
Reported most in Body
Documents 36
Total Number 52
Disease Relevance 17.22
Pain Relevance 0.39

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

pigmentation (Mitf) cell differentiation (Mitf) nucleus (Mitf)
protein complex assembly (Mitf) DNA binding (Mitf) protein complex (Mitf)
Anatomy Link Frequency
melanocyte 4
uterus 3
mast cells 2
embryo 1
muscle 1
Mitf (Mus musculus)
Pain Link Frequency Relevance Heat
wide dynamic range 17 99.68 Very High Very High Very High
Inflammation 43 99.60 Very High Very High Very High
melanocortin 1 receptor 32 91.92 High High
palliative 27 62.32 Quite High
Inflammatory response 7 52.24 Quite High
metalloproteinase 95 40.96 Quite Low
qutenza 1 25.00 Low Low
depression 26 5.00 Very Low Very Low Very Low
anesthesia 19 5.00 Very Low Very Low Very Low
Central nervous system 8 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Microphthalmia 53 100.00 Very High Very High Very High
Melanoma 1902 99.84 Very High Very High Very High
Skin Cancer 69 99.84 Very High Very High Very High
INFLAMMATION 54 99.60 Very High Very High Very High
Congenital Anomalies 2 99.58 Very High Very High Very High
Metastasis 340 99.16 Very High Very High Very High
Experimental Melanoma 27 98.68 Very High Very High Very High
Cancer 1174 97.96 Very High Very High Very High
Targeted Disruption 9 97.84 Very High Very High Very High
Stress 53 97.80 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
For example, MITF was shown to bind and transactivate the HIF1a promoter in mouse B16 melanoma cells [29], yet in our study HIF1a was inversely correlated with MITF expression, particularly in the invasive cell lines, suggesting de-regulated MITF and/or cAMP signalling in NZM cells, or that B16 cells may not be a good model of human metastatic melanoma.
Gene_expression (expression) of MITF associated with melanoma and experimental melanoma
1) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.56 Pain Relevance 0
MITF was reported to be regulated by ERK-activating BRAF mutations [20], so we investigated whether differential MITF expression could be explained by using CD200 as a proxy for ERK activation [21].
Gene_expression (expression) of MITF
2) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.20 Pain Relevance 0
DCT, CDK2, BCL2, GPR143) despite having relatively higher MITF expression (Figure 1B, C), indicating that there are probably melanoma sub-types present within the broad Motif 1 and 2 classifications, and that not all cell lines with lower MITF have a Motif 1 expression profile.


Gene_expression (expression) of MITF associated with melanoma
3) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.31 Pain Relevance 0
Thirty-five of the genes in our 96-gene invasion signature are known or predicted targets of MITF [18], and although experimentally confirmed MITF targets such as MLANA, RAB27A, and GPR143 corresponded closely with MITF expression in our data, some did not, suggesting a disconnect in MITF signalling for some MITF targets in certain melanomas.
Gene_expression (expression) of MITF associated with melanoma
4) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.63 Pain Relevance 0
Genomic amplification of the MITF locus, which may occur in 10–15% of metastatic melanomas [23], was unlikely to underlie the differential expression of MITF between our Motif 1 and 2 NZM cell lines as there was no difference in MITF copy number between NZM cell lines with varying levels of relative MITF gene expression (Figure S3).


Gene_expression (expression) of MITF associated with melanoma
5) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.33 Pain Relevance 0
Motif 1 cell lines showed a 23-fold higher capacity for migration in transwell assays than Motif 2 cell lines (Figure 2A), and were significantly faster at wound repair in scratch assays than Motif 2 cell lines (Figure 2B; Movie S1, Movie S2). siRNA-mediated MITF knockdown in weakly invasive NZM06 and NZM15 cells caused an average 4-fold increase in migration in transwell assays (Figure 2C; Figure S2), confirming that relative MITF expression was central to the observed difference in invasive potential between Motif 1 and 2 cell lines, and consistent with Motif 1 representing an invasion signature for melanoma cells in vitro.
Gene_expression (expression) of MITF associated with melanoma and injury
6) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.19 Pain Relevance 0
Genomic amplification of the MITF locus, which may occur in 10–15% of metastatic melanomas [23], was unlikely to underlie the differential expression of MITF between our Motif 1 and 2 NZM cell lines as there was no difference in MITF copy number between NZM cell lines with varying levels of relative MITF gene expression (Figure S3).


Gene_expression (expression) of MITF associated with melanoma
7) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.31 Pain Relevance 0
In accordance with the microarray, qPCR, and western blot data, immunofluoresence suggested differential expression of both MITF and MLANA in representative Motif 1 and 2 NZM cells, with strong nuclear signal for MITF and strong cytoplasmic signal for MLANA in NZM06 compared to NZM09 (Figure 1D).


Gene_expression (expression) of MITF
8) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.12 Pain Relevance 0
MITF transcript levels did not correlate with CD200 expression in NZM cell lines (Figure S3), whereas MLANA, a transcriptional target of MITF, strongly correlated with MITF expression as expected (Figure S3).
Gene_expression (expression) of MITF
9) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.20 Pain Relevance 0
We found that MITF transcript levels did not correlate with CD200, a proxy of ERK activation, which agrees with the conclusions of others that if ERK activation caused by, for example, BRAF mutation, regulates MITF expression in melanoma then it is only weakly so at the level of transcription.
Gene_expression (expression) of MITF associated with melanoma
10) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.80 Pain Relevance 0
This is consistent with the work of other investigators that showed reduced levels of MITF was associated with increased invasiveness in melanoma cells [27], whereas MITF over-expression suppressed melanoma metastasis in mouse xenograft tumours [28].
Gene_expression (over) of MITF associated with cancer, melanoma and metastasis
11) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 1.35 Pain Relevance 0
A number of mechanisms have been postulated to alter MITF expression in melanoma.
Gene_expression (expression) of MITF associated with melanoma
12) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 1.19 Pain Relevance 0
Further, expression of the POU3F2 (BRN2) transcription factor, which was reported to be a negative regulator of MITF expression [22], did not inversely correlate with MITF transcript levels in NZM cell lines (Figure S3).
Gene_expression (expression) of MITF
13) Confidence 0.75 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.15 Pain Relevance 0
Le Pape et al. (2009) have also now reported a valuable microarray analysis of gene expression changes in melan-a cells grown with ASIP, which supports the concept of partial dedifferentiation and complements the present findings; for example reduced expression of a large number of pigmentary genes including Mitf was noted after growth with ASIP.
Gene_expression (expression) of Mitf
14) Confidence 0.72 Published 2009 Journal Pigment Cell & Melanoma Research Section Body Doc Link PMC2784899 Disease Relevance 0 Pain Relevance 0.07
The mitf gene is reported to be expressed in various tissues containing skeletal muscle, heart muscle, and mast cells [18], but no marked defects have been observed in the skeletal muscles of mi/mi and mitf null mice [13].
Gene_expression (expressed) of mitf in mast cells
15) Confidence 0.68 Published 2010 Journal The Open Dentistry Journal Section Body Doc Link PMC2835863 Disease Relevance 0.07 Pain Relevance 0
In contrast, there was no significant increase detectable in the mesentery of MITF-mutants, from which bone marrow-derived cultured mast cells (BMMCs) lack SgIGSF.
Neg (lack) Gene_expression (lack) of MITF in bone marrow
16) Confidence 0.65 Published 2007 Journal J. Neuroimmunol. Section Abstract Doc Link 17250899 Disease Relevance 0.17 Pain Relevance 0
We found that MITF transcript levels did not correlate with CD200, a proxy of ERK activation, which agrees with the conclusions of others that if ERK activation caused by, for example, BRAF mutation, regulates MITF expression in melanoma then it is only weakly so at the level of transcription.
Gene_expression (levels) of MITF associated with melanoma
17) Confidence 0.65 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.95 Pain Relevance 0
In accordance with the microarray, qPCR, and western blot data, immunofluoresence suggested differential expression of both MITF and MLANA in representative Motif 1 and 2 NZM cells, with strong nuclear signal for MITF and strong cytoplasmic signal for MLANA in NZM06 compared to NZM09 (Figure 1D).


Gene_expression (expression) of MITF
18) Confidence 0.65 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.17 Pain Relevance 0
Global transcript profiling identified a signature featuring decreased expression of developmental and lineage specification genes including MITF, EDNRB, DCT, and TYR, and increased expression of genes involved in interaction with the extracellular environment, such as PLAUR, VCAN, and HIF1a.
Gene_expression (expression) of MITF
19) Confidence 0.58 Published 2009 Journal PLoS ONE Section Abstract Doc Link PMC2794539 Disease Relevance 0.68 Pain Relevance 0
Global gene expression analysis showed that the cell lines could be stratified by differential expression of genes related to melanocyte development and differentiation, and that lower expression of MITF and related transcriptional networks combined with higher expression of environmental interaction genes correlated with increased invasive potential in vitro.
Gene_expression (expression) of MITF in melanocyte
20) Confidence 0.58 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2794539 Disease Relevance 0.78 Pain Relevance 0

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