INT97693

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Context Info
Confidence 0.75
First Reported 2001
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 29
Total Number 32
Disease Relevance 5.51
Pain Relevance 5.26

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (REST) nucleolus (REST) nucleus (REST)
intracellular (REST) DNA binding (REST) transcription factor binding (REST)
Anatomy Link Frequency
Neuron 5
neuronal 2
nerve 2
HeLa 2
brain 1
REST (Homo sapiens)
Pain Link Frequency Relevance Heat
dorsal root ganglion 6 99.68 Very High Very High Very High
Calcium channel 154 99.12 Very High Very High Very High
Opioid 31 99.08 Very High Very High Very High
Nerve growth factor 15 98.80 Very High Very High Very High
opioid receptor 11 96.08 Very High Very High Very High
sodium channel 38 94.96 High High
c fibre 8 90.24 High High
Neuronal excitability 3 82.80 Quite High
Neuropathic pain 12 80.92 Quite High
Peripheral nerve injury 2 79.96 Quite High
Disease Link Frequency Relevance Heat
Nervous System Injury 8 99.74 Very High Very High Very High
Ganglion Cysts 6 99.68 Very High Very High Very High
Small Cell Lung Cancer 16 99.16 Very High Very High Very High
Infection 15 98.96 Very High Very High Very High
Stress 205 98.92 Very High Very High Very High
Repression 45 98.64 Very High Very High Very High
Neuroblastoma 12 97.48 Very High Very High Very High
Neuropathic Pain 24 80.92 Quite High
Phobia 3 79.56 Quite High
Ataxia 14 78.04 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
On the other hand, in neuronal PC12 cells, the promoter activity of the CRH gene is down-regulated by the overexpression of REST/NRSF.
Gene_expression (overexpression) of NRSF in neuronal
1) Confidence 0.75 Published 2008 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2733102 Disease Relevance 0 Pain Relevance 0
When RE-1/NRSE is disrupted, the basal promoter activity of the CRH gene is robustly elevated in myoblast-derived L6 cells in which REST/NRSF is abundantly expressed, compared with the wild-type construct (Seth and Majzoub, 2001).
Gene_expression (expressed) of NRSF in myoblast
2) Confidence 0.75 Published 2008 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2733102 Disease Relevance 0 Pain Relevance 0
However, the splice variant of REST/NRSF, produced in several kinds of SCLC cells, interferes with the normal silencing function of wild-type REST/NRSF, facilitating the ectopic expression of the vasopressin gene in the heterologous cells (Coulson et al. 1999b).
Gene_expression (produced) of NRSF associated with small cell lung cancer
3) Confidence 0.75 Published 2008 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2733102 Disease Relevance 0.10 Pain Relevance 0
Thus, the promoter activity is stimulated in this mutant when REST/NRSF expression vector is introduced, indicating that REST/NRSF acts as an enhancer through the RE-1/NRSF-independent pathway, depending on the types of cells or tissues.


Gene_expression (expression) of NRSF
4) Confidence 0.65 Published 2008 Journal Gene Regulation and Systems Biology Section Body Doc Link PMC2733102 Disease Relevance 0 Pain Relevance 0
Overexpression of NRSF led to both increased opioid ligand-binding activity of the endogenous MOR and MOR-GFP fusion protein expression.
Gene_expression (Overexpression) of NRSF associated with opioid
5) Confidence 0.60 Published 2008 Journal Biochim. Biophys. Acta Section Abstract Doc Link 18657578 Disease Relevance 0.07 Pain Relevance 0.10
When MOR and NRSF genes were coexpressed, the specific ligand-binding activity of MOR was increased in neuroblastoma NMB cells, but decreased in PC12 cells result from its localization.
Gene_expression (coexpressed) of NRSF associated with neuroblastoma
6) Confidence 0.60 Published 2008 Journal Biochim. Biophys. Acta Section Abstract Doc Link 18657578 Disease Relevance 0.10 Pain Relevance 0.09
Overexpression of NRSF also led to enhanced phosphorylation of eIF4G.
Gene_expression (Overexpression) of NRSF
7) Confidence 0.60 Published 2008 Journal Biochim. Biophys. Acta Section Abstract Doc Link 18657578 Disease Relevance 0.09 Pain Relevance 0.11
In order to investigate whether these weaker sites can recruit REST at higher cellular concentrations, similar ChIP assays were carried out on HeLa cells overexpressing virally delivered, full-length REST protein.
Gene_expression (overexpressing) of REST in HeLa
8) Confidence 0.60 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0
In contrast to wild-type cells, all four CACNA1A RE1s were found to be occupied in cells overexpressing REST.
Gene_expression (overexpressing) of REST
9) Confidence 0.60 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0
HeLa cells are non-neuronal and express relatively high levels of REST [L.
Gene_expression (express) of REST in neuronal
10) Confidence 0.60 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0.09 Pain Relevance 0.08
Recombinant adenoviruses expressing transgenes for REST DNA-binding domain (Ad DN:REST), or full-length REST (Ad REST), or no transgene (Ad), were amplified in HEK293 cells and purified by centrifugation in a CsCl gradient as described in Ref. (38).
Gene_expression (expressing) of REST
11) Confidence 0.60 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0.10 Pain Relevance 0
The gene is classified in the RE1-enriched ‘voltage-gated calcium channel complex’ ontology classification, and is a paralogue of the REST target CACNA1H.
Gene_expression (paralogue) of REST associated with calcium channel
12) Confidence 0.60 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0.10
The three non-conserved human RE1s we tested by ChIP (CACNB2, CACNA1G, CACNG7) recruit REST in vivo, suggesting that these are functional sites (Figure 5D).
Gene_expression (tested) of REST
13) Confidence 0.60 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0.08
This resonates with the model of Ballas et al. (45) regarding the progressive loss of REST from target genes during neuronal differentiation, where REST recruitment is lost from those genes with weaker RE1s first, as REST levels in the nucleus drop during development.
Gene_expression (levels) of REST in nucleus
14) Confidence 0.53 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0.09 Pain Relevance 0
Indeed, after overexpressing NRSF in NMB cells, the target RNA moved to the translationally active polysomal fraction.
Gene_expression (overexpressing) of NRSF
15) Confidence 0.53 Published 2008 Journal Biochim. Biophys. Acta Section Abstract Doc Link 18657578 Disease Relevance 0.10 Pain Relevance 0.11
Intriguingly, many neuronal genes with rapidly evolving coding sequences have been identified as REST targets by this study (65), while evolutionary changes in brain developmental processes, in which REST plays a central role, have been important drivers of brain evolution (66).
Gene_expression (targets) of REST in brain
16) Confidence 0.52 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0
Dissecting the REST-regulatory sequences of a model gene, CACNA1A
Gene_expression (sequences) of REST-regulatory
17) Confidence 0.52 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0.03
We also apply the RE1 PSSM to the exhaustive analysis of the REST-regulatory apparatus of a model gene, the novel target CACNA1A.
Gene_expression (apparatus) of REST-regulatory
18) Confidence 0.52 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0.31 Pain Relevance 0.20
Dissecting the REST-regulatory sequences of a model gene, CACNA1A
Gene_expression (Dissecting) of REST-regulatory
19) Confidence 0.52 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0 Pain Relevance 0.03
Recombinant adenoviruses expressing transgenes for REST DNA-binding domain (Ad DN:REST), or full-length REST (Ad REST), or no transgene (Ad), were amplified in HEK293 cells and purified by centrifugation in a CsCl gradient as described in Ref. (38).
Gene_expression (expressing) of REST
20) Confidence 0.47 Published 2006 Journal Nucleic Acids Research Section Body Doc Link PMC1557810 Disease Relevance 0.10 Pain Relevance 0

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