INT98687

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Context Info
Confidence 0.61
First Reported 2001
Last Reported 2010
Negated 2
Speculated 0
Reported most in Body
Documents 38
Total Number 38
Disease Relevance 7.03
Pain Relevance 1.59

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

unfolded protein binding (Calr) Golgi apparatus (Calr) endoplasmic reticulum (Calr)
cytoplasm (Calr) cytosol (Calr) protein folding (Calr)
Anatomy Link Frequency
nucleus 3
muscles 1
SCN 1
muscle fibers 1
myotube 1
Calr (Mus musculus)
Pain Link Frequency Relevance Heat
gABA 2 100.00 Very High Very High Very High
Inflammation 245 99.42 Very High Very High Very High
Glutamate 20 99.22 Very High Very High Very High
Neuropeptide 2 95.16 Very High Very High Very High
Enkephalin 1 94.52 High High
5HT 1 93.32 High High
anesthesia 37 83.28 Quite High
ischemia 3 82.16 Quite High
ketamine 20 74.08 Quite High
Calcium channel 76 70.88 Quite High
Disease Link Frequency Relevance Heat
Neuroma 79 99.84 Very High Very High Very High
Myositis 294 99.64 Very High Very High Very High
INFLAMMATION 205 98.28 Very High Very High Very High
Stress 232 98.18 Very High Very High Very High
Cancer 34 95.68 Very High Very High Very High
Injury 12 94.16 High High
Necrosis 16 93.76 High High
Myocardial Infarction 4 91.96 High High
Apoptosis 12 91.04 High High
Vibrio Infection 1 89.24 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
GDH (cytosolic marker), CZ (lysosomal marker) and CRT (ER marker) were detected in the soluble fractions by Western blot using the appropriate antibodies.


Gene_expression (detected) of CRT
1) Confidence 0.61 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
PatCRT underwent the CRT procedure to resynchronise the contraction of the LV.
Gene_expression (procedure) of CRT
2) Confidence 0.56 Published 2006 Journal Biomed Eng Online Section Body Doc Link PMC1636054 Disease Relevance 0.22 Pain Relevance 0.08
The aim of the present study was therefore to investigate the stress-response ongoing in muscle fibers of patients affected with inflammatory myopathies, focusing on Grp94, calreticulin and Grp75 expression and distribution.
Gene_expression (expression) of calreticulin in muscle fibers associated with stress and inflammation
3) Confidence 0.48 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2888201 Disease Relevance 0.60 Pain Relevance 0.12
CRT was immunoprecipitated and biotinylated CRT was detected by Western blot using Streptavidin-HRP.
Gene_expression (detected) of CRT
4) Confidence 0.47 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
subunit, ERp57 (a member of the protein disulfide isomerase family) and calreticulin (CRT) can also be found in the cytoplasm and nucleus.
Gene_expression (found) of calreticulin in nucleus
5) Confidence 0.47 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0.05 Pain Relevance 0
subunit, ERp57 (a member of the protein disulfide isomerase family) and calreticulin (CRT) can also be found in the cytoplasm and nucleus.
Gene_expression (found) of CRT in nucleus
6) Confidence 0.47 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0.05 Pain Relevance 0
Lower cytosolic TcCRT levels were observed in the presence of cycloheximide, thus suggesting that TcCRT was being degraded in the cytosol.


Gene_expression (levels) of TcCRT
7) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Aborted ER translocation would operate during the first steps of protein synthesis and the C-terminal-truncated TcCRT and the full length protein should be expected to behave similarly.
Gene_expression (expected) of TcCRT
8) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Although CRT resides mainly in the ER lumen, several reports have found it in other locations such as cytosol, nucleus, secretory granules, the outer side of the plasma membrane and the extracellular space.
Gene_expression (resides) of CRT in nucleus
9) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
The effect exerted by ER calcium was strictly dependent on the C-terminal domain (CRT-C), since a CRT lacking it was totally retained in the ER, whereas the localization of an unrelated protein fused to CRT-C mirrored that of endogenous CRT.
Gene_expression (retained) of CRT
10) Confidence 0.46 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2950133 Disease Relevance 0.16 Pain Relevance 0
The amount of cytosolic TcCRT reached a maximum ?
Gene_expression (amount) of TcCRT
11) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
We also found that a shorter version of TcCRT lacking the C-terminal domain was totally retained in the ER, whereas a non related protein such as GFP fused to this same domain mimicked TcCRT localization.
Gene_expression (retained) of TcCRT
12) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0.03
Only a very small proportion of TcCRT was biotinylated, probably due to a low fraction of dead, lysed parasites.
Gene_expression (proportion) of TcCRT
13) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Similar changes of cytosolic TcCRT were observed in the presence or absence of the protein synthesis inhibitor (Figure 2B and D), thus arguing in favor of the retrotranslocation hypothesis.
Gene_expression (changes) of TcCRT
14) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
In both cases the endogenous TcCRT appeared in both compartments, with a distribution similar to that observed in non-transfected parasites.
Gene_expression (appeared) of TcCRT
15) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
The full length HA-tagged TcCRT appeared in both cytosolic and ER fractions, whereas the protein lacking the C-terminal domain was visible only in the last fraction, as revealed by Western blot using anti-TcCRT antibodies (Figure 3B).
Gene_expression (appeared) of TcCRT
16) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
In contrast, the presence of TcCRT was evident in both fractions (Figure 1A).
Gene_expression (presence) of TcCRT
17) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Sulfo-NHS-biotin was also employed to asses the proportion of TcCRT present in the lysosomal and ER fractions.
Gene_expression (present) of TcCRT
18) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
The presence of cytosolic CRT in mammalian cells depends on CRT-C [31].
Gene_expression (presence) of CRT
19) Confidence 0.46 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2950133 Disease Relevance 0 Pain Relevance 0
Different patterns of immunohistochemical labeling for Grp94, Grp75 and calreticulin were detected in biopsies from the patient groups (Figures 1 and 2).
Gene_expression (detected) of calreticulin
20) Confidence 0.41 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2888201 Disease Relevance 0.34 Pain Relevance 0

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