INT113021

Context Info Confidence 0.37 First Reported 2003 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 2 Total Number 5 Disease Relevance 2.94 Pain Relevance 4.49

cell differentiation (ADAM18)

Anatomy Link Frequency tubule 1 ADAM18 (Homo sapiens) ADAM18 - L858F (4)

Pain Link Frequency Relevance Heat Nav1.7 392 100.00 Pain 46 94.24 addiction 24 89.16 sodium channel 40 82.00 burning pain 8 71.12 chronic pain syndrome 8 67.60 dorsal root ganglion 40 66.60 IPN 4 61.88 neuralgia 2 10.00 nav1.8 16 5.00

Disease Link Frequency Relevance Heat Erythermalgia 108 100.00 Pain 44 94.24 Disease 8 88.40 Ganglion Cysts 48 86.12 Nociception 8 84.52 Neuropathic Pain 1 79.52 Pressure And Volume Under Development 1 78.36 Non-diabetic Peripheral Neuropathy 1 76.28 Increased Venous Pressure Under Development 8 72.60 Complex Regional Pain Syndromes 8 67.60

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Double staining confocal microscope studies using anti-human protein-gene-product 9.5, anti-tubule, anti-neurofilament, and anti-synaptophysin antibodies were performed to assess specific accumulation within swellings.

Gene_expression (using) of protein-gene-product 9.5 in tubule

1) Confidence 0.37 Published 2003 Journal Neurology Section Body Doc Link 12963753 Disease Relevance 0.23 Pain Relevance 0

Whole-cell voltage-clamp measurements on wild type or L858F channels expressed in HEK293 cells revealed that cooling decreases current density, slows deactivation and increases ramp currents for both mutant and wild type channels.

Gene_expression (expressed) of L858F (L858F)

2) Confidence 0.01 Published 2007 Journal Mol Pain Section Abstract Doc Link PMC1781932 Disease Relevance 0.93 Pain Relevance 0.77

Whole-cell pa</span>tch-clamp recordings of sodium currents from HEK293 cells stably expressing WT or the IEM mutant Nav1.7 channel (L858F) were carried out at three different temperatures: 16°C, 25°C and 35°C.

Gene_expression (expressing) of L858F (L858F) associated with erythermalgia and nav1.7

3) Confidence 0.01 Published 2007 Journal Mol Pain Section Body Doc Link PMC1781932 Disease Relevance 1.03 Pain Relevance 1.47

As shown previously [7] L858F produces larger ramp currents than WT channels.

Gene_expression (produces) of L858F (L858F) associated with nav1.7

4) Confidence 0.01 Published 2007 Journal Mol Pain Section Body Doc Link PMC1781932 Disease Relevance 0 Pain Relevance 0.92

Both WT and L858F mutant channels produced fast activating and inactivating currents (Figure 1A).

Gene_expression (produced) of L858F (L858F) associated with nav1.7

5) Confidence 0.01 Published 2007 Journal Mol Pain Section Body Doc Link PMC1781932 Disease Relevance 0.75 Pain Relevance 1.32

General Comments

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