INT124569

Context Info Confidence 0.15 First Reported 2005 Last Reported 2009 Negated 0 Speculated 3 Reported most in Abstract Documents 6 Total Number 8 Disease Relevance 1.25 Pain Relevance 3.32

cytosol (HPD) small molecule metabolic process (HPD) Golgi apparatus (HPD) endoplasmic reticulum (HPD) cellular nitrogen compound metabolic process (HPD)

Anatomy Link Frequency hypothalamus 5 preoptic area 1 ovary 1 HPD (Homo sapiens)

Pain Link Frequency Relevance Heat Dynorphin 45 99.60 Morphine 12 95.36 Kappa opioid receptor 2 88.08 Endogenous opioid 5 78.16 Opioid 6 78.08 drug abuse 2 70.04 cytokine 29 33.44 opiate 2 25.00 Inflammation 12 21.28 Angina 1 5.00

Disease Link Frequency Relevance Heat Infection 55 98.22 Mycobacterial Infection 70 90.56 Stress 5 84.48 Drug Dependence 2 70.04 Latent Tuberculosis 6 50.00 INFLAMMATION 13 21.28 Lymphatic Filariasis 7 5.00 Worm Infection 4 5.00 Disease 4 5.00 Autoimmune Disease 2 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

To detect PPD mRNA, an ovine PPD mRNA was subcloned by reverse transcription-polymerase chain reaction from sheep hypothalamus and used to create a (35)S-labeled riboprobe for in situ hybridization.

Transcription (detect) of PPD in hypothalamus

1) Confidence 0.15 Published 2005 Journal Neuroscience Section Abstract Doc Link 15664697 Disease Relevance 0.08 Pain Relevance 0.40

Neurons that expressed PPD mRNA and DYN A immunoreactivity were widely distributed in the ovine preoptic area and hypothalamus.

Transcription (expressed) of PPD in hypothalamus associated with dynorphin

2) Confidence 0.15 Published 2005 Journal Neuroscience Section Abstract Doc Link 15664697 Disease Relevance 0.07 Pain Relevance 0.41

To detect PPD mRNA, an ovine PPD mRNA was subcloned by reverse transcription-polymerase chain reaction from sheep hypothalamus and used to create a (35)S-labeled riboprobe for in situ hybridization.

Transcription (detect) of PPD in hypothalamus

3) Confidence 0.15 Published 2005 Journal Neuroscience Section Abstract Doc Link 15664697 Disease Relevance 0.08 Pain Relevance 0.40

In this study, we determined the distribution of PPD mRNA and DYN A-containing cell bodies in the brains of ovary-intact, luteal ewes.

Transcription (distribution) of PPD in ovary associated with dynorphin

4) Confidence 0.15 Published 2005 Journal Neuroscience Section Abstract Doc Link 15664697 Disease Relevance 0.08 Pain Relevance 0.38

Neurons that expressed PPD mRNA and DYN A immunoreactivity were widely distributed in the ovine preoptic area and hypothalamus.

Transcription (expressed) of PPD in preoptic area associated with dynorphin

5) Confidence 0.05 Published 2005 Journal Neuroscience Section Abstract Doc Link 15664697 Disease Relevance 0.07 Pain Relevance 0.41

To determine the impact of coexisting filarial infection on baseline and antigen-specific TLR expression of PPD+ individuals, and because filarial infection is associated with diminished expression of TLR1, 2, 4, and 9 [3], we examined the mRNA expression of these TLRs from PPD+Fil?

Spec (examined) Transcription (expression) of PPD associated with infection

6) Confidence 0.02 Published 2009 Journal PLoS Neglected Tropical Diseases Section Body Doc Link PMC2710006 Disease Relevance 0.72 Pain Relevance 0

Several weeks later, quantitative RT-PCR was used to determine mRNA expression within the MBH in diestrus, never pregnant (nulliparous) controls, postpartum day 5 (PPD5), PPD10, PPD18, and diestrus, reproductively experienced (primiparous) females.

Spec (determine) Transcription (expression) of PPD18 in MBH

7) Confidence 0.00 Published 2008 Journal Brain Res. Section Abstract Doc Link 18083149 Disease Relevance 0.07 Pain Relevance 0.66

Several weeks later, quantitative RT-PCR was used to determine mRNA expression within the MBH in diestrus, never pregnant (nulliparous) controls, postpartum day 5 (PPD5), PPD10, PPD18, and diestrus, reproductively experienced (primiparous) females.

Spec (determine) Transcription (expression) of PPD10 in MBH

8) Confidence 0.00 Published 2008 Journal Brain Res. Section Abstract Doc Link 18083149 Disease Relevance 0.07 Pain Relevance 0.66

General Comments

This test has worked.