INT127009

Context Info Confidence 0.70 First Reported 2005 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 5 Total Number 8 Disease Relevance 5.30 Pain Relevance 9.90

transport (Slc12a2) plasma membrane (Slc12a2) transmembrane transport (Slc12a2)

Anatomy Link Frequency spinal cord 6 spinal 4 plasma 4 visceral 2 Slc12a2 (Mus musculus)

Pain Link Frequency Relevance Heat Dorsal horn 28 100.00 Spinal cord 24 100.00 qutenza 148 99.88 Pain 72 99.82 allodynia 320 99.02 Hyperalgesia 76 98.32 spinal dorsal horn 12 97.04 GABAergic 24 94.20 Neuronal excitability 4 93.40 intrathecal 120 88.92

Disease Link Frequency Relevance Heat Neuropathic Pain 324 99.02 Hyperalgesia 92 98.32 Targeted Disruption 12 96.46 Pain 36 87.64 Nociception 16 87.36 INFLAMMATION 60 86.24 Peripheral Arterial Disease 16 75.44 Ganglion Cysts 28 67.12 Injury 4 54.64 Dyspepsia 8 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Painful stimuli induce in vivo phosphorylation and membrane mobilization of mouse spinal cord NKCC1 co-transporter.

Positive_regulation (induce) of Phosphorylation (phosphorylation) of NKCC1 in spinal cord associated with pain and spinal cord

1) Confidence 0.70 Published 2005 Journal Neuroscience Section Title Doc Link 15893647 Disease Relevance 0.33 Pain Relevance 0.98

Finally, intracolonic capsaicin injection stimulates a rapid and transient increase in spinal phosphorylated NKCC1 and a long lasting increase in trafficking of NKCC1 protein to the plasma membrane [11].

Positive_regulation (increase) of Phosphorylation (phosphorylated) of NKCC1 in plasma associated with qutenza

2) Confidence 0.64 Published 2007 Journal Mol Pain Section Body Doc Link PMC1929059 Disease Relevance 0.74 Pain Relevance 1.25

These results suggest that phosphorylation and recruitment of NKCC1 might play a role in referred mechanical hyperalgesia evoked by a painful visceral stimulus.

Positive_regulation (recruitment) of Phosphorylation (phosphorylation) of NKCC1 in visceral associated with pain and hyperalgesia

3) Confidence 0.50 Published 2005 Journal Neuroscience Section Abstract Doc Link 15893647 Disease Relevance 0.33 Pain Relevance 1.06

Intracolonic capsaicin also evoked a rapid (10 min) and transient phosphorylation of NKCC1, however, intracolonic saline did not produce significant changes in either NKCC1 trafficking or phosphorylation and none of the treatments induced any alterations of NKCC1 in the thoracic spinal cord.

Positive_regulation (evoked) of Phosphorylation (phosphorylation) of NKCC1 in spinal cord associated with qutenza and spinal cord

4) Confidence 0.50 Published 2005 Journal Neuroscience Section Abstract Doc Link 15893647 Disease Relevance 0.36 Pain Relevance 1.12

Intracolonic capsaicin also evoked a rapid (10 min) and transient phosphorylation of NKCC1, however, intracolonic saline did not produce significant changes in either NKCC1 trafficking or phosphorylation and none of the treatments induced any alterations of NKCC1 in the thoracic spinal cord.

Positive_regulation (induced) of Phosphorylation (alterations) of NKCC1 in spinal cord associated with qutenza and spinal cord

5) Confidence 0.50 Published 2005 Journal Neuroscience Section Abstract Doc Link 15893647 Disease Relevance 0.34 Pain Relevance 1.09

-fiber input that is GABAergic in nature [13], 2) stroking allodynia following capsaicin injection into the hindpaw is absent in NKCC1 knockout mice [8], 3) spinal application of the NKCC1 blocker BUM inhibits intraplantar capsaicin-evoked, secondary mechanical allodynia in rats [10] and 4) a painful visceral stimulus increases dorsal horn NKCC1 phosphorylation and membrane trafficking [11], both of which are mechanisms known to increase NKCC1 activity and intracellular Cl- concentrations [1,26].

Positive_regulation (increases) of Phosphorylation (phosphorylation) of NKCC1 in spinal associated with targeted disruption, pain, qutenza, allodynia, gabaergic and dorsal horn

6) Confidence 0.46 Published 2007 Journal Mol Pain Section Body Doc Link PMC1929059 Disease Relevance 0.93 Pain Relevance 1.27

-fiber input that is GABAergic in nature [13], 2) stroking allodynia following capsaicin injection into the hindpaw is absent in NKCC1 knockout mice [8], 3) spinal application of the NKCC1 blocker BUM inhibits intraplantar capsaicin-evoked, secondary mechanical allodynia in rats [10] and 4) a painful visceral stimulus increases dorsal horn NKCC1 phosphorylation and membrane trafficking [11], both of which are mechanisms known to increase NKCC1 activity and intracellular Cl- concentrations [1,26].

Positive_regulation (increase) of Phosphorylation (phosphorylation) of NKCC1 in spinal associated with targeted disruption, pain, qutenza, allodynia, gabaergic and dorsal horn

7) Confidence 0.46 Published 2007 Journal Mol Pain Section Body Doc Link PMC1929059 Disease Relevance 0.95 Pain Relevance 1.29

Work from this laboratory has shown previously that an intracolonic capsaicin injection causes a transient increase in NKCC1 phosphorylation and a sustained increase in plasma membrane localization of NKCC1 in the spinal dorsal horn [11].

Positive_regulation (increase) of Phosphorylation (phosphorylation) of NKCC1 in plasma associated with qutenza and spinal dorsal horn

8) Confidence 0.43 Published 2007 Journal Mol Pain Section Body Doc Link PMC1929059 Disease Relevance 1.32 Pain Relevance 1.84

General Comments

This test has worked.