INT127500

Context Info Confidence 0.78 First Reported 2005 Last Reported 2010 Negated 1 Speculated 2 Reported most in Abstract Documents 79 Total Number 81 Disease Relevance 24.58 Pain Relevance 23.30

Anatomy Link Frequency sensory neurons 5 neurons 5 nerve 4 spinal nerve 3 trigeminal ganglia 3 TRPA1 (Homo sapiens)

Pain Link Frequency Relevance Heat antagonist 1069 100.00 agonist 903 100.00 Pain 726 100.00 Trigeminal ganglion neurons 1 99.92 nociceptor 24 99.84 Cold hyperalgesia 186 99.44 Hyperalgesia 16 99.44 dysesthesia 21 99.42 c fibre 6 98.88 Inflammation 204 98.72

Disease Link Frequency Relevance Heat Neuroma 51 99.84 Nociception 32 99.84 Ganglion Cysts 133 99.76 Hyperalgesia 206 99.44 Dysesthesia 21 99.42 Targeted Disruption 296 99.34 Pain 692 99.20 Injury 89 98.88 INFLAMMATION 99 98.72 Neurogenic Inflammation 121 97.84

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

TRPA1 is an ion channel expressed by nociceptors and activated by irritant compounds such as mustard oil.

Gene_expression (expressed) of TRPA1 in nociceptors associated with nociceptor

1) Confidence 0.78 Published 2007 Journal Nat. Neurosci. Section Abstract Doc Link 17259981 Disease Relevance 0 Pain Relevance 0.10

TRPA1 expression in human lingual nerve neuromas in patients with and without symptoms of dysaesthesia.

Gene_expression (expression) of TRPA1 in nerve associated with pain, dysesthesia and neuroma

2) Confidence 0.78 Published 2009 Journal Neurosci. Lett. Section Title Doc Link 19715741 Disease Relevance 0.83 Pain Relevance 0.51

No significant difference between levels of TRPA1 in neuromas from patients with or without symptoms of dysaesthesia and no relationship between TRPA1 expression and VAS scores for pain, tingling or discomfort were observed.

Gene_expression (levels) of TRPA1 associated with pain, dysesthesia and neuroma

3) Confidence 0.78 Published 2009 Journal Neurosci. Lett. Section Abstract Doc Link 19715741 Disease Relevance 1.07 Pain Relevance 0.45

No significant difference between levels of TRPA1 in neuromas from patients with or without symptoms of dysaesthesia and no relationship between TRPA1 expression and VAS scores for pain, tingling or discomfort were observed.

Gene_expression (expression) of TRPA1 associated with pain, dysesthesia and neuroma

4) Confidence 0.78 Published 2009 Journal Neurosci. Lett. Section Abstract Doc Link 19715741 Disease Relevance 1.07 Pain Relevance 0.46

We examined expression of TRPA1 in lingual nerve neuromas and investigated any potential correlation with the presence or absence of symptoms of dysaesthesia.

Spec (examined) Gene_expression (expression) of TRPA1 in nerve associated with dysesthesia and neuroma

5) Confidence 0.78 Published 2009 Journal Neurosci. Lett. Section Abstract Doc Link 19715741 Disease Relevance 0.75 Pain Relevance 0.26

These data show that TRPA1 is expressed in lingual nerve neuromas, but, it appears that, at this site, TRPA1 does not play a principal role in the development of neuropathic pain.

Gene_expression (expressed) of TRPA1 in nerve associated with neuropathic pain and neuroma

6) Confidence 0.78 Published 2009 Journal Neurosci. Lett. Section Abstract Doc Link 19715741 Disease Relevance 1.11 Pain Relevance 0.45

TRPA1 expression and the time after initial injury that the specimen was obtained also showed no correlation.

Gene_expression (expression) of TRPA1 associated with injury

7) Confidence 0.78 Published 2009 Journal Neurosci. Lett. Section Abstract Doc Link 19715741 Disease Relevance 1.06 Pain Relevance 0.45

TRPA1 is sufficient to mediate responses to CO(2) as TRPA1 channels expressed in HEK-293 cells, but not TRPV1 channels, were activated by bath-applied CO(2).

Gene_expression (expressed) of TRPA1

8) Confidence 0.78 Published 2010 Journal J. Neurosci. Section Abstract Doc Link 20881114 Disease Relevance 0.07 Pain Relevance 0.08

Here we show that CO(2) specifically activates a subpopulation of trigeminal neurons that express TRPA1, a mustard oil- and cinnamaldehyde-sensitive channel, and that these responses are dependent on a functional TRPA1 gene.

Gene_expression (express) of TRPA1 in neurons

9) Confidence 0.78 Published 2010 Journal J. Neurosci. Section Abstract Doc Link 20881114 Disease Relevance 0.07 Pain Relevance 0.09

TRPA1 is a member of the transient receptor potential (TRP) cation channel family, and is predominantly expressed in nociceptive neurons of dorsal root ganglia (DRG) and trigeminal ganglia.

Gene_expression (expressed) of TRPA1 in trigeminal ganglia associated with nociception

10) Confidence 0.78 Published 2008 Journal Channels (Austin) Section Abstract Doc Link 18769139 Disease Relevance 0.47 Pain Relevance 0.26

Here, we show that a variety of inflammatory mediators (15-deoxy-Delta(12,14)-prostaglandin J(2) (15d-PGJ(2)), nitric oxide (NO), hydrogen peroxide (H(2)O(2)), and proton (H(+))) activate human TRPA1 heterologously expressed in HEK cells.

Gene_expression (expressed) of TRPA1 associated with inflammatory mediators

11) Confidence 0.78 Published 2008 Journal Channels (Austin) Section Abstract Doc Link 18769139 Disease Relevance 0.58 Pain Relevance 0.34

MEASUREMENTS: The expression patterns of TRPA1 were studied with Western blot and immunohistochemistry.

Gene_expression (expression) of TRPA1

12) Confidence 0.78 Published 2009 Journal Eur. Urol. Section Body Doc Link 18468780 Disease Relevance 0.08 Pain Relevance 0

The active ingredient in mustard oil, allyl isothiocyanate (AITC) selectively activates a non-selective cation channel, transient receptor potential ankyrin 1 (TRPA1) expressed in the small neurons of the dorsal root and trigeminal ganglia [4,5].

Gene_expression (expressed) of ankyrin 1 in dorsal root

13) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0.26 Pain Relevance 0.31

We then characterized both human and rat TRPA1 expression in CHO cells by measuring AITC-induced increases in intracellular calcium and the ability to inhibit this response by ruthenium red.

Gene_expression (expression) of TRPA1

14) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0.65 Pain Relevance 0.69

In order to screen the small molecule libraries, CHO cells expressing human TRPA1 seeded in 96-well plates were first incubated with 10 ?

Gene_expression (expressing) of TRPA1

15) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0 Pain Relevance 0.13

Maximum response was observed in TRPA1 expressing CHO cells incubated at 3.5–4.2°C.

Gene_expression (expressing) of TRPA1

16) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0 Pain Relevance 0.07

Minimal induction of 45Ca2+ uptake by untrasfected CHO cells was observed at noxious cold (3–5 to 7°C), however cells expressing TRPA1 showed a four to five-fold higher activation at the same temperatures in this assay (Fig. 2C).

Gene_expression (expressing) of TRPA1

17) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0 Pain Relevance 0.06

To evaluate if noxious cold activates TRPA1 channels in our experimental conditions, we employed a radioactive calcium uptake assay in native CHO cells as well as CHO cells that express TRPA1 and confirmed that noxious cold indeed activates both human and rat TRPA1.

Gene_expression (express) of TRPA1

18) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0.47 Pain Relevance 0.48

Before evaluating TCEB compounds ability to inhibit rat TRPA1 activation, we tested their potential agonism in CHO cells expressing TRPA1.

Gene_expression (expressing) of TRPA1

19) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0 Pain Relevance 0.20

We compared 45Ca2+ uptake in CHO cells expressing human TRPA1 and in untransfected CHO cells exposed to temperatures between 3.5 and 25°C.

Gene_expression (expressing) of TRPA1

20) Confidence 0.77 Published 2007 Journal Mol Pain Section Body Doc Link PMC2222611 Disease Relevance 0 Pain Relevance 0.07

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