INT134747

Context Info Confidence 0.72 First Reported 2004 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 10 Total Number 10 Disease Relevance 0.44 Pain Relevance 3.48

transmembrane transporter activity (SV2A) endoplasmic reticulum (SV2A) cytoplasm (SV2A)

Anatomy Link Frequency row 1 CAR 1 brain 1 SV2A (Homo sapiens)

Pain Link Frequency Relevance Heat opioid receptor 64 99.86 substance P 24 97.52 cytokine 2 83.56 Inflammation 2 83.20 Neuropeptide 6 81.28 Morphine 8 79.20 GABAergic 7 75.72 Opioid 4 75.00 Calcitonin gene-related peptide 11 67.84 Neurotransmitter 32 67.76

Disease Link Frequency Relevance Heat Neuroblastoma 4 99.60 INFLAMMATION 2 83.20 Generalized Anxiety Disorder 19 5.00 Colon Cancer 8 5.00 Disease 5 5.00 Liver Cancer 4 5.00 Rheumatoid Arthritis 3 5.00 Epilepsy 1 5.00 Dyskinesias 1 5.00 Injury 1 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The eukaryotic expression plasmid for CAR(SV5) was constructed by cloning the 910 bp EcoRI/XmaI insert fragment of pcDhCAR(SV1) and the 130 bp XmaI/HindIII insert fragment of a pGEM exon 8–9 clone (see above) containing the deletion in exon 9 into appropriately digested vector pcDNA3.1(-) (Invitrogen).

Gene_expression (expression) of SV5 in CAR

1) Confidence 0.72 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0

In NMB cells expressing human MOR gene, transfection of SV1 or SV2 reduced binding activity of the endogenous MOR.

Gene_expression (transfection) of SV2 associated with opioid receptor

2) Confidence 0.56 Published 2006 Journal Biochem. Biophys. Res. Commun. Section Abstract Doc Link 16580639 Disease Relevance 0.08 Pain Relevance 0.69

In a receptor binding assay, cells expressing the SV1 and SV2 do not exhibit binding to [(3)H]diprenorphine.

Gene_expression (expressing) of SV2

3) Confidence 0.56 Published 2006 Journal Biochem. Biophys. Res. Commun. Section Abstract Doc Link 16580639 Disease Relevance 0.10 Pain Relevance 0.81

Thus we obtained eukaryotic expression plasmids (pcDhCAR) for transcripts SV1, SV2, SV4 and SV6.

Gene_expression (expression) of SV4

4) Confidence 0.56 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0

Thus we obtained eukaryotic expression plasmids (pcDhCAR) for transcripts SV1, SV2, SV4 and SV6.

Gene_expression (expression) of SV2

5) Confidence 0.56 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0

Thus we obtained eukaryotic expression plasmids (pcDhCAR) for transcripts SV1, SV2, SV4 and SV6.

Gene_expression (expression) of SV6

6) Confidence 0.56 Published 2004 Journal Nucl Recept Section Body Doc Link PMC406421 Disease Relevance 0 Pain Relevance 0

SV1 and SV2 of hMOR were present in human neuroblastoma NMB cells and human whole brain confirmed by RT-PCR.

Gene_expression (present) of SV2 in brain associated with neuroblastoma

7) Confidence 0.50 Published 2006 Journal Biochem. Biophys. Res. Commun. Section Abstract Doc Link 16580639 Disease Relevance 0.10 Pain Relevance 0.77

In vitro translation of SV1 and SV2 produced proteins with the predicted molecular weights.

Gene_expression (produced) of SV2

8) Confidence 0.50 Published 2006 Journal Biochem. Biophys. Res. Commun. Section Abstract Doc Link 16580639 Disease Relevance 0.08 Pain Relevance 0.67

As shown in Figure 2, quantitative analysis of SVOP labeling showed saturation with an apparent Kd value of 83 µM, a value within physiological concentrations of ATP in cells, and similar to the apparent Kd values measured for SV2 (?

Gene_expression (measured) of SV2

9) Confidence 0.50 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2669215 Disease Relevance 0 Pain Relevance 0

D12 cells expressed both synaptic proteins (SV2 –second row, first panel; Syn –fourth row, first panel), and SP (second and fourth rows, second panel), with co-localization throughout the cell body and neurites (Merge –second and fourth rows, fourth panel).

Gene_expression (expressed) of SV2 in row associated with substance p

10) Confidence 0.33 Published 2008 Journal Biological Procedures Online Section Body Doc Link PMC2683550 Disease Relevance 0.08 Pain Relevance 0.55

General Comments

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