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Context Info
Confidence 0.75
First Reported 2006
Last Reported 2010
Negated 0
Speculated 1
Reported most in Body
Documents 8
Total Number 32
Disease Relevance 6.74
Pain Relevance 8.05

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

signal transduction (P2rx4) transport (P2rx4) plasma membrane (P2rx4)
Anatomy Link Frequency
microglia 9
C8-B4 6
plasma 3
vesicle 2
spinal cord 1
P2rx4 (Mus musculus)
Pain Link Frequency Relevance Heat
Endep 400 99.54 Very High Very High Very High
Spinal cord 45 99.34 Very High Very High Very High
Neuropathic pain 302 99.28 Very High Very High Very High
allodynia 42 98.88 Very High Very High Very High
tetrodotoxin 2 98.56 Very High Very High Very High
sodium channel 2 97.72 Very High Very High Very High
antidepressant 875 97.64 Very High Very High Very High
Eae 25 97.56 Very High Very High Very High
antagonist 154 96.96 Very High Very High Very High
IPN 7 96.40 Very High Very High Very High
Disease Link Frequency Relevance Heat
Neuropathic Pain 387 99.28 Very High Very High Very High
Injury 13 98.30 Very High Very High Very High
Nervous System Injury 54 98.28 Very High Very High Very High
Spinal Cord Injury 5 97.64 Very High Very High Very High
Inflammatory Pain 7 96.40 Very High Very High Very High
INFLAMMATION 28 96.32 Very High Very High Very High
Hypertrophy 1 95.16 Very High Very High Very High
Hypersensitivity 14 94.56 High High
Pain 69 93.88 High High
Targeted Disruption 26 91.68 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
These results indicate functional expression of P2X4 receptors in cortical neurones and their involvement in purinergic synaptic transmission in cortex.
Gene_expression (expression) of P2X4 in cortex
1) Confidence 0.75 Published 2007 Journal Neurosci. Lett. Section Abstract Doc Link 17566648 Disease Relevance 0 Pain Relevance 0.37
Ivermectin potentiates ATP-induced ion currents in cortical neurones: evidence for functional expression of P2X4 receptors?
Gene_expression (expression) of P2X4
2) Confidence 0.75 Published 2007 Journal Neurosci. Lett. Section Title Doc Link 17566648 Disease Relevance 0 Pain Relevance 0.29
Eighth, we provide the first measure for the Pf% for a natively expressed P2X4 receptor.
Gene_expression (expressed) of P2X4
3) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.09 Pain Relevance 0.25
Before proceeding further, we tested the effect of the Src family tyrosine kinase inhibitor PP2 (20 µM for 4 h with LPS) because previous work shows that this reduces P2X4 expression in microglia (Tsuda et al., 2008).
Gene_expression (expression) of P2X4 in microglia
4) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
Resting C8-B4 cells expressed virtually no functional P2X receptors, but largely increased functional expression of P2X4 receptors within 2–6 h of entering the activated state.
Gene_expression (expression) of P2X4 in C8-B4
5) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Abstract Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
Cognizant of the aforementioned limitations of using immunocytochemistry, we used surface biotinylation of membrane proteins to obtain more direct data to support our hypothesis that C8-B4 cell activation results in elevated P2X4 expression in the plasma membrane.
Gene_expression (expression) of P2X4 in plasma
6) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
Moreover, in the study by Raouf et al. (2007), the microglia used expressed both P2X4 and P2X7 receptors.
Gene_expression (expressed) of P2X4 in microglia
7) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.08 Pain Relevance 0.17
Analysis of P2X4 subunit protein expression in resting and activated C8-B4 cells
Gene_expression (expression) of P2X4 subunit in C8-B4
8) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
This limitation is increased by the fact that available P2X4 antibodies target a cytosolic domain epitope and require cell permeabilization, rendering the increase in plasma membrane expression of P2X4 difficult to detect against a much larger cytosolic component.
Gene_expression (expression) of P2X4 in plasma
9) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
Third, we found no significant ATP-evoked currents in resting cells, but we detected small but clear surface expression of P2X4 proteins by biotinylation, suggesting that some P2X4 receptors in the plasma membrane of resting C8-B4 cells were nonfunctional.
Gene_expression (expression) of P2X4 in C8-B4
10) Confidence 0.67 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.06 Pain Relevance 0.08
In contrast to the neuropathic pain model, neither the upregulation of P2X4R expression [7] nor activation of Lyn tyrosine kinase [13] has been demonstrated in spinal microglia following peripheral inflammation caused by CFA.
Gene_expression (expression) of P2X4R in microglia associated with inflammation and neuropathic pain
11) Confidence 0.60 Published 2009 Journal Mol Pain Section Body Doc Link PMC2704200 Disease Relevance 1.38 Pain Relevance 0.98
In a later section, we examine surface P2X4 expression in resting and activated microglia using surface biotinylation.


Spec (examine) Gene_expression (expression) of P2X4 in microglia
12) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.09 Pain Relevance 0
Surface biotinylation of P2X4 receptors
Gene_expression (receptors) of P2X4
13) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0.04
We also observed P2X4 immunostaining in larger vesicle-like structures that were reminiscent of lysosomes (Qureshi et al., 2007; Stokes and Surprenant, 2009).
Gene_expression (immunostaining) of P2X4 in vesicle
14) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0
Early studies showed that P2X4 receptors were widely expressed in the brain (Lê et al., 1998b; Rubio and Soto, 2001), whereas more recent studies show that they are also expressed in microglia and alveolar macrophages (Bowler et al., 2003; Tsuda et al., 2003; Raouf et al., 2007; Ulmann et al., 2008; Stokes and Surprenant, 2009).
Gene_expression (expressed) of P2X4 in microglia
15) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0.07
Second, how are P2X4 receptors up-regulated in microglia?
Gene_expression (receptors) of P2X4 in microglia
16) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.32 Pain Relevance 0.37
Thus, P2X4-mediated responses desensitized over seconds, displayed inwardly rectifying current–voltage relations that reversed at ?
Gene_expression (desensitized) of P2X4
17) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.18 Pain Relevance 0.24
These data therefore favor P2X2, P2X5, P2X4, or P2X7 receptors (Surprenant and North, 2009).
Gene_expression (favor) of P2X4
18) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0 Pain Relevance 0.05
Similarities and differences to past work with P2X4 receptors in immune cells
Gene_expression (receptors) of P2X4 in immune cells
19) Confidence 0.58 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.09 Pain Relevance 0.22
Thus, strategies that reduce or abolish P2X4 expression alleviate symptoms such as allodynia that are a hallmark of neuropathic pain models (Tsuda et al., 2003; Ulmann et al., 2008).
Gene_expression (expression) of P2X4 associated with allodynia and eae
20) Confidence 0.52 Published 2010 Journal The Journal of General Physiology Section Body Doc Link PMC2847917 Disease Relevance 0.37 Pain Relevance 0.40

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