INT144010

Context Info Confidence 0.78 First Reported 2005 Last Reported 2007 Negated 1 Speculated 0 Reported most in Abstract Documents 11 Total Number 11 Disease Relevance 2.40 Pain Relevance 0.66

nucleus (Hspb6) response to stress (Hspb6) cytoplasm (Hspb6)

Anatomy Link Frequency myometrium 3 muscle 2 longitudinal muscle 1 Hspb6 (Rattus norvegicus)

Pain Link Frequency Relevance Heat Dismenorea 9 97.32 Glutamate 144 92.08 Morphine 1 31.32 anesthesia 3 5.00 ketamine 2 5.00 Spinal cord 1 5.00 Nerve growth factor 1 5.00 ischemia 1 5.00 Neurotransmitter 1 5.00 Hippocampus 1 5.00

Disease Link Frequency Relevance Heat Shock 18 100.00 Dysmenorrhea 9 97.32 Death 68 92.84 Ganglion Cysts 3 87.92 Parkinson's Disease 1 87.52 Apoptosis 68 78.64 Osteoporosis 1 40.72 Neurodegenerative Disease 6 5.00 Adhesions 4 5.00 Necrosis 4 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Heat shock protein 20 (HSP20) is known to be present at high levels in smooth muscle and implicated in muscle relaxation, but HSP20 expression in the myometrium is completely undetermined.

Gene_expression (expression) of HSP20 in muscle associated with shock

1) Confidence 0.78 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.27 Pain Relevance 0.09

Expression of small heat shock-related protein 20 (HSP20) in rat myometrium is markedly decreased during late pregnancy and labour.

Gene_expression (Expression) of HSP20 in myometrium associated with shock

2) Confidence 0.78 Published 2007 Journal Reproduction Section Title Doc Link 17504924 Disease Relevance 0.29 Pain Relevance 0.10

Since HSP20 has been implicated in smooth muscle relaxation, we hypothesized that HSP20 would be highly expressed in the rat myometrium during early and mid-pregnancy when the myometrium is relatively quiescent.

Gene_expression (expressed) of HSP20 in myometrium

3) Confidence 0.78 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.25 Pain Relevance 0.09

Heat shock protein 20 (HSP20) is known to be present at high levels in smooth muscle and implicated in muscle relaxation, but HSP20 expression in the myometrium is completely undetermined.

Gene_expression (expression) of HSP20 in muscle associated with shock

4) Confidence 0.78 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.27 Pain Relevance 0.10

The observed patterns of HSP20 expression are consistent with a potential role for HSP20 in facilitating myometrium quiescence during early and mid-pregnancy.

Gene_expression (expression) of HSP20 in myometrium

5) Confidence 0.78 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.07 Pain Relevance 0

Our results demonstrate that HSP20 mRNA and protein are highly expressed during early and mid-pregnancy and then the expression markedly decreases during late pregnancy and labour.

Gene_expression (expressed) of HSP20 mRNA

6) Confidence 0.78 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.07 Pain Relevance 0

Immunoblot analysis showed that detection of HSP20 was significantly decreased at d23 compared with d12 and d15 (P < 0.05).

Gene_expression (detection) of HSP20

7) Confidence 0.68 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.21 Pain Relevance 0.07

Northern blot analysis particularly demonstrated that HSP20 mRNA detection was significantly decreased from day (d) 22 of pregnancy to 1-day post-partum (PP) compared with d6 (P < 0.05).

Gene_expression (detection) of HSP20 mRNA

8) Confidence 0.68 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.24 Pain Relevance 0.08

In addition, HSP20 was detectable near cell membranes at much higher levels in the longitudinal muscle layer of progesterone-treated pregnant rats (delayed labour) at all gestational time points examined, compared with controls.

Gene_expression (detectable) of HSP20 in longitudinal muscle

9) Confidence 0.60 Published 2007 Journal Reproduction Section Abstract Doc Link 17504924 Disease Relevance 0.19 Pain Relevance 0.05

Although we were unable to directly detect by Western blot analysis, the active caspase-3 fragments p20 and p11, the presence of active caspase-3 was confirmed as discussed above.

Neg (unable) Gene_expression (detect) of p20

10) Confidence 0.20 Published 2005 Journal BMC Neurosci Section Body Doc Link PMC555946 Disease Relevance 0.45 Pain Relevance 0.08

We next focused on the general pattern of CD81, EBP50 and Sap97, at two time points, P2 (Figure 6) and P20 (Figure 5).

Gene_expression (pattern) of P20

11) Confidence 0.15 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2610370 Disease Relevance 0.09 Pain Relevance 0

General Comments

This test has worked.