INT146241

Context Info Confidence 0.69 First Reported 2007 Last Reported 2010 Negated 3 Speculated 1 Reported most in Body Documents 23 Total Number 25 Disease Relevance 8.07 Pain Relevance 8.74

transport (Trpa1) transmembrane transport (Trpa1)

Anatomy Link Frequency nociceptor 8 neurons 4 HEK 4 nerve 2 IB4 2 Trpa1 (Mus musculus)

Pain Link Frequency Relevance Heat nociceptor 171 100.00 agonist 120 100.00 Morphine 83 99.98 TRP channel 124 99.76 Neuropathic pain 57 99.40 Pain 286 99.34 Eae 38 98.32 antagonist 79 98.20 Cold hyperalgesia 18 98.16 Inflammation 104 97.00

Disease Link Frequency Relevance Heat Neuroblastoma 2 99.84 Neuropathic Pain 179 99.40 Pain 278 99.34 Hypersensitivity 50 99.00 Hyperalgesia 121 97.96 Targeted Disruption 116 97.10 INFLAMMATION 101 97.00 Nociception 148 96.48 Injury 147 96.32 Nervous System Injury 63 96.32

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Similarly, a recent study showed that overexpression of TRPA1 in neuroblastoma cell lines also does not confer novel mechanical sensitivity or induce any changes in endogenous mechanically-evoked currents in these heterologous cells [27].

Positive_regulation (overexpression) of Gene_expression (overexpression) of TRPA1 associated with neuroblastoma

1) Confidence 0.69 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2922334 Disease Relevance 0.17 Pain Relevance 0.09

It was argued that this selective increase in TRPA1 expression in the uninjured L4 DRG underlied the emergence of cold hypersensitivity.

Positive_regulation (increase) of Gene_expression (expression) of TRPA1 associated with hypersensitivity

2) Confidence 0.66 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2753652 Disease Relevance 0.56 Pain Relevance 0.29

In a subclass of mouse nociceptors expressing TRPA1 channels, assessed by responses to the TRPA1 agonist mustard oil, nifedipine also produced large elevations in [Ca2+](i).

Positive_regulation (elevations) of Gene_expression (expressing) of TRPA1 in nociceptors associated with nociceptor and agonist

3) Confidence 0.50 Published 2008 Journal Channels (Austin) Section Abstract Doc Link 18971630 Disease Relevance 0.16 Pain Relevance 0.45

In a subclass of mouse nociceptors expressing TRPA1 channels, assessed by responses to the TRPA1 agonist mustard oil, nifedipine also produced large elevations in [Ca2+](i).

Positive_regulation (elevations) of Gene_expression (expressing) of TRPA1 in nociceptors associated with nociceptor and agonist

4) Confidence 0.50 Published 2008 Journal Channels (Austin) Section Abstract Doc Link 18971630 Disease Relevance 0.16 Pain Relevance 0.45

Transfection of TRPA1 in HEK293 cells did not significantly alter the proportion or magnitude of mechanically-activated currents in HEK293 cells, indicating that TRPA1 alone is not sufficient to confer mechanical sensitivity.These parallel genetic and pharmacological data demonstrate that TRPA1 mediates the Slowly Adapting mechanically-activated currents in small-diameter IB4 negative neurons from adult mice.

Positive_regulation (Transfection) of Gene_expression (Transfection) of TRPA1 in IB4

5) Confidence 0.50 Published 2010 Journal PLoS ONE Section Abstract Doc Link 20808441 Disease Relevance 0.05 Pain Relevance 0.19

Expression of TRPA1 in HEK293 cells does not significantly increase mechanically-evoked currents

Neg (not) Positive_regulation (increase) of Gene_expression (Expression) of TRPA1

6) Confidence 0.50 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2922334 Disease Relevance 0 Pain Relevance 0

This suggests that cold hypersensitivity in neuropathic pain is not always dependent upon increased expression of TRPA1 and TRPM8 in DRG.

Positive_regulation (increased) of Gene_expression (expression) of TRPA1 associated with hypersensitivity and neuropathic pain

7) Confidence 0.48 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2753652 Disease Relevance 0.68 Pain Relevance 0.28

KEY FINDINGS: The 3 terpenoids activated TRPA1 that was heterologously expressed in HEK293 or CHO cells.

Positive_regulation (activated) of Gene_expression (expressed) of TRPA1

8) Confidence 0.46 Published 2009 Journal Life Sci. Section Body Doc Link 19409911 Disease Relevance 0 Pain Relevance 0

RESULTS: Irritant inhaled anesthetics activate TRPA1 expressed in human embryonic kidney cells and in nociceptive neurons.

Positive_regulation (activate) of Gene_expression (expressed) of TRPA1 in neurons

9) Confidence 0.46 Published 2010 Journal Anesthesiology Section Body Doc Link 20463581 Disease Relevance 0.08 Pain Relevance 0

Previous studies have shown that robust activation of TRPV1 can desensitize TRPA1 [48], but also that TRPV1 is required for proper TRPA1 expression [40].

Positive_regulation (required) of Gene_expression (expression) of TRPA1

10) Confidence 0.45 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2764051 Disease Relevance 0.36 Pain Relevance 0.07

Both cisplatin and oxaliplatin-treated mice displayed significant reductions in mechanical pain thresholds and also showed high levels of TRPA1 expression (11 fold and 6.7 fold, respectively).

Positive_regulation (levels) of Gene_expression (expression) of TRPA1 associated with pain

11) Confidence 0.44 Published 2010 Journal Mol Pain Section Body Doc Link PMC2848188 Disease Relevance 1.15 Pain Relevance 0.81

In this study, using real-time, reverse transcriptase, polymerase chain reaction (RT-PCR), we analyzed the expression of TRPV1, TRPM8, and TRPA1 induced by cisplatin or oxaliplatin in vitro and in vivo.

Spec (analyzed) Positive_regulation (induced) of Spec (analyzed) Gene_expression (expression) of TRPA1

12) Confidence 0.44 Published 2010 Journal Mol Pain Section Abstract Doc Link PMC2848188 Disease Relevance 1.03 Pain Relevance 0.69

We found that clinically relevant CLT concentrations activate heterologously expressed TRPV1 and TRPA1, two TRP channels that act as receptors of irritant chemical and/or thermal stimuli in nociceptive neurons.

Positive_regulation (activate) of Gene_expression (expressed) of TRPA1 in neurons associated with nociception and trp channel

13) Confidence 0.44 Published 2008 Journal J. Neurosci. Section Abstract Doc Link 18199759 Disease Relevance 0.43 Pain Relevance 0.37

Among 17 TRP channels expressed in HEK cells, HalphaSS caused Ca(2+) influx in cells transfected with TRPV1 or TRPA1, and evoked robust inward currents in cells transfected with TRPV1 or TRPA1.

Positive_regulation (transfected) of Gene_expression (transfected) of TRPA1 in HEK associated with trp channel

14) Confidence 0.44 Published 2007 Journal Eur. J. Neurosci. Section Abstract Doc Link 17767493 Disease Relevance 0 Pain Relevance 0.14

Among 17 TRP channels expressed in HEK cells, HalphaSS caused Ca(2+) influx in cells transfected with TRPV1 or TRPA1, and evoked robust inward currents in cells transfected with TRPV1 or TRPA1.

Positive_regulation (transfected) of Gene_expression (transfected) of TRPA1 in HEK associated with trp channel

15) Confidence 0.44 Published 2007 Journal Eur. J. Neurosci. Section Abstract Doc Link 17767493 Disease Relevance 0 Pain Relevance 0.14

The observation suggests that ret signalling is not required for the generation of a TRPV1-positive nociceptor subclass but for the expression of an additional differentiation marker, TRPA1.

Neg (not) Positive_regulation (required) of Gene_expression (expression) of TRPA1 in nociceptor associated with nociceptor

16) Confidence 0.40 Published 2008 Journal Cell Tissue Res Section Body Doc Link PMC2516536 Disease Relevance 0 Pain Relevance 0.42

Correspondingly, transgenic animals show no difference in behavioural response to mechanical stimulation but an increased heat and cold immersion response correlating with increased TRPV1 and TRPA1 expression, respectively.

Positive_regulation (increased) of Gene_expression (expression) of TRPA1 associated with targeted disruption

17) Confidence 0.40 Published 2008 Journal Cell Tissue Res Section Body Doc Link PMC2516536 Disease Relevance 0.38 Pain Relevance 0.42

When TRPA1 is overexpressed in CHO cells, it can be activated by micromolar concentrations of cinnamaldehyde, that is, an increase in intracellular Ca2+ concentration was observed [6].

Positive_regulation (overexpressed) of Gene_expression (overexpressed) of TRPA1

18) Confidence 0.39 Published 2008 Journal Current Neuropharmacology Section Body Doc Link PMC2645550 Disease Relevance 0.22 Pain Relevance 0.25

M menthol significantly inhibits both the cold-induced rise of [Ca2+]cyt as cold-induced currents in TRPA1 transfected CHO cells [65].

Positive_regulation (transfected) of Gene_expression (transfected) of TRPA1

19) Confidence 0.39 Published 2008 Journal Current Neuropharmacology Section Body Doc Link PMC2645550 Disease Relevance 0.13 Pain Relevance 0.19

When TRPA1 is overexpressed in CHO cells, it can be activated by micromolar concentrations of cinnamaldehyde, that is, an increase in intracellular Ca2+ concentration was observed [6].

Positive_regulation (activated) of Gene_expression (overexpressed) of TRPA1

20) Confidence 0.39 Published 2008 Journal Current Neuropharmacology Section Body Doc Link PMC2645550 Disease Relevance 0.22 Pain Relevance 0.28

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