INT159140

Context Info Confidence 0.61 First Reported 2007 Last Reported 2010 Negated 0 Speculated 2 Reported most in Body Documents 11 Total Number 13 Disease Relevance 5.04 Pain Relevance 0.50

Golgi apparatus (Tjp1) plasma membrane (Tjp1) nucleus (Tjp1) cytoplasm (Tjp1)

Anatomy Link Frequency colon 4 epithelial cells 2 Tjp1 (Mus musculus)

Pain Link Frequency Relevance Heat anesthesia 9 93.44 Inflammation 130 79.92 Inflammatory response 16 68.52 tolerance 4 63.28 Inflammatory mediators 21 43.32 Crohn's disease 28 36.24 Versed 14 24.16 cytokine 14 5.00 tetrodotoxin 7 5.00 cINOD 4 5.00

Disease Link Frequency Relevance Heat Brain Injury 11 100.00 Infection 192 99.44 Colitis 217 98.52 Adhesions 10 95.04 Sepsis 1 93.68 Inflammatory Bowel Disease 119 91.92 Body Weight 51 88.84 Injury 9 87.80 Brain Disease 1 87.36 INFLAMMATION 156 79.92

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Whilst we observed increased expression of Tjp1 at the transcriptional level in NS398 treated infected mice, Jcam expression was not affected.

Positive_regulation (increased) of Gene_expression (expression) of Tjp1

1) Confidence 0.61 Published 2009 Journal Mol Cancer Section Body Doc Link PMC2667483 Disease Relevance 0.37 Pain Relevance 0.09

Together with the influence of EcN on intestinal permeability and an enhanced ZO-1 expression under pathological conditions, it can be speculated that EcN augments mucosal barrier function.

Positive_regulation (enhanced) of Gene_expression (expression) of ZO-1

2) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.53 Pain Relevance 0

To analyze whether the improved state of health after EcN treatment is accompanied with an increased ZO-1 expression, IECs were isolated from the colon of treated mice and analyzed for ZO-1 gene expression.

Spec (whether) Positive_regulation (increased) of Gene_expression (expression) of ZO-1 in colon

3) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.67 Pain Relevance 0.10

Using another murine colitis model, administration of n-3 polyunsaturated fatty acids resulted not only in reduced pathological scores but also an increase of ZO-1 protein expression [56].

Positive_regulation (increase) of Gene_expression (expression) of ZO-1 protein associated with colitis

4) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.48 Pain Relevance 0

Elevated ZO-1 expression after EcN treatment in experimental colitis

Positive_regulation (Elevated) of Gene_expression (expression) of ZO-1 associated with colitis

5) Confidence 0.49 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.48 Pain Relevance 0

We hypothesized that expression of tight junction protein ZO-1 and occludin, responsible for intestinal architectural and functional integrity, will decrease following TBI and increase intestinal permeability.

Positive_regulation (increase) of Gene_expression (expression) of ZO-1 associated with brain injury

6) Confidence 0.48 Published 2009 Journal J. Neurotrauma Section Abstract Doc Link 19344293 Disease Relevance 1.04 Pain Relevance 0.09

In comparison to IECs isolated from control mice and those colonized with E. coli MG1655, IECs from EcN treated animals showed a markedly elevated expression of ZO-1 protein (Figure 3B).

Positive_regulation (elevated) of Gene_expression (expression) of ZO-1 protein

7) Confidence 0.43 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0 Pain Relevance 0

In NS398 treated mice, the expression of Tjp1 dramatically increased in the early stages of the study compared to infected mice (Figure 3).

Positive_regulation (increased) of Gene_expression (expression) of Tjp1

8) Confidence 0.41 Published 2009 Journal Mol Cancer Section Body Doc Link PMC2667483 Disease Relevance 0.36 Pain Relevance 0.08

This idea is supported by our in vitro observations of a cox-2 dependent increase in Zo-1 protein expression in MKN28 cells.

Positive_regulation (increase) of Gene_expression (expression) of Zo-1 protein

9) Confidence 0.41 Published 2009 Journal Mol Cancer Section Body Doc Link PMC2667483 Disease Relevance 0.22 Pain Relevance 0

Western blot analysis showed that infection with H. pylori also led to increases in Zona occludens 1(ZO1, Human homologue to Tjp1) protein expression in vitro in MKN28 gastric epithelial cells, and that this increase was inhibited in the presence of NS398 (Figure 4A).

Positive_regulation (increases) of Gene_expression (expression) of ZO1 in epithelial cells associated with infection

10) Confidence 0.41 Published 2009 Journal Mol Cancer Section Body Doc Link PMC2667483 Disease Relevance 0.27 Pain Relevance 0.03

To analyze whether the improved state of health after EcN treatment is accompanied with an increased ZO-1 expression, IECs were isolated from the colon of treated mice and analyzed for ZO-1 gene expression.

Spec (analyzed) Positive_regulation (analyzed) of Spec (analyzed) Gene_expression (expression) of ZO-1 in colon

11) Confidence 0.35 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.57 Pain Relevance 0.10

ZO-1 mRNA and protein expression are elevated in gnotobiotic mice colonized with EcN

Positive_regulation (elevated) of Gene_expression (expression) of ZO-1 mRNA

12) Confidence 0.33 Published 2007 Journal PLoS ONE Section Body Doc Link PMC2110898 Disease Relevance 0.06 Pain Relevance 0

The expression level of ZO-1 was increased after treatment with PCB118 or PCB153, without any changes in PCB126-exposed animals.

Positive_regulation (increased) of Gene_expression (expression) of ZO-1

13) Confidence 0.12 Published 2010 Journal Environ Health Perspect Section Body Doc Link PMC2854723 Disease Relevance 0 Pain Relevance 0

General Comments

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