INT165963

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Context Info
Confidence 0.75
First Reported 2008
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 37
Total Number 37
Disease Relevance 20.68
Pain Relevance 0.66

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (Map2k2) kinase activity (Map2k2)
Anatomy Link Frequency
IEC-6 8
colon 6
SW480 3
epithelial cells 2
lung 2
Map2k2 (Mus musculus)
Pain Link Frequency Relevance Heat
metalloproteinase 245 97.52 Very High Very High Very High
Analgesic 4 70.52 Quite High
Perioperative pain 35 36.44 Quite Low
isoflurane 35 24.56 Low Low
Inflammation 5 5.00 Very Low Very Low Very Low
chemokine 5 5.00 Very Low Very Low Very Low
cytokine 3 5.00 Very Low Very Low Very Low
Inflammatory response 3 5.00 Very Low Very Low Very Low
colic 1 5.00 Very Low Very Low Very Low
headache 1 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Cancer 1260 99.98 Very High Very High Very High
Targeted Disruption 141 99.82 Very High Very High Very High
Colon Cancer 1575 99.66 Very High Very High Very High
Intestinal Cancer 280 99.24 Very High Very High Very High
Apoptosis 285 99.04 Very High Very High Very High
Metastasis 350 98.64 Very High Very High Very High
Adenocarcinoma 280 95.36 Very High Very High Very High
Adhesions 70 95.00 High High
Injury 35 93.00 High High
Infection 264 92.72 High High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The activation levels of ERK, the p38 target MK-2 and the JNK target c-jun in cell lysates were assessed by immunoblotting with phospho-specific antibodies.
Gene_expression (levels) of MK-2
1) Confidence 0.75 Published 2010 Journal BMC Microbiol Section Body Doc Link PMC3022711 Disease Relevance 0.07 Pain Relevance 0
However, ectopic expression or MEK2DD slightly increased the steady-state levels of endogenous MEK1, while overexpression of MEK1DD had a similar effect on MEK2 levels (Fig. 1B).
Gene_expression (expression) of MEK2DD
2) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.40 Pain Relevance 0
Strikingly, lowering of MEK2 expression with the two shRNAs completely suppressed the proliferation of HCT116 cells, whereas MEK1 shRNAs exerted a significant but much weaker effect (Fig. 6B).
Gene_expression (expression) of MEK2
3) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.11 Pain Relevance 0
The ability of activated MEK1- or MEK2-expressing tumor cells to colonize distant organs was associated with increased invasiveness, secretion of matrix proteases and resistance to anoikis.
Gene_expression (expressing) of MEK2 associated with cancer
4) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 1.34 Pain Relevance 0
Of the 69 gene transcripts that showed altered expression in the study of Komatsu, 18 (26%) were found to be modulated in IEC-6 cells expressing constitutively active MEK1 or MEK2.
Gene_expression (expressing) of MEK2 in IEC-6
5) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.43 Pain Relevance 0.07
This knowledge is important since both MEK1 and MEK2 are expressed in intestinal epithelial cells and immunohistochemistry analysis with phospho-specific MEK1/2 antibodies does not allow to discriminate between the two isoforms.
Gene_expression (expressed) of MEK2 in epithelial cells
6) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.57 Pain Relevance 0
Here, we show that expression of activated MEK1 or MEK2 not only induces the formation of intestinal tumors but also promotes later stages of tumor progression and metastasis to distant organs.
Gene_expression (expression) of MEK2 associated with cancer, intestinal cancer and metastasis
7) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 1.57 Pain Relevance 0
Expression of the urokinase receptor was also up-regulated in IEC-6 cells expressing activated MEK2.
Gene_expression (expressing) of MEK2 in IEC-6
8) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.34 Pain Relevance 0.18
MEK1 and MEK2 display 85% amino acid identity overall and are expressed ubiquitously in cell lines and tissues.
Gene_expression (expressed) of MEK2
9) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.81 Pain Relevance 0
Reciprocally, induction of the BH3-only pro-apoptotic protein Bim was completely suppressed in cells expressing MEK1DD or MEK2DD (Fig. 5C).
Gene_expression (expressing) of MEK2DD associated with apoptosis
10) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.70 Pain Relevance 0
Most importantly, we found that silencing of MEK2 expression (even if not total) completely suppressed the proliferation of human colon carcinoma cell lines, whereas complete knock-down of MEK1 had a much weaker effect.
Gene_expression (expression) of MEK2 in colon associated with targeted disruption and colon cancer
11) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.60 Pain Relevance 0.04
Similar to HCT116 cells, knock-down of MEK2 expression dramatically suppressed the proliferation of SW480 cells, whereas MEK1 silencing induced a significant but much lower decrease of cell proliferation (Fig. 6C).
Gene_expression (expression) of MEK2 in SW480 associated with targeted disruption
12) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.36 Pain Relevance 0
No major difference was observed in the growth rate of tumors expressing activated MEK1 or MEK2 (Fig. 2D).
Gene_expression (expressing) of MEK2 associated with cancer
13) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.58 Pain Relevance 0
Ectopic expression of activated MEK1 or MEK2 significantly enhanced the invasive capacity of IEC-6 cells, while the wild type MEK isoforms had no effect (Fig. 3B).
Gene_expression (expression) of MEK2 in IEC-6
14) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.48 Pain Relevance 0
Silencing of MEK2 expression markedly inhibits the proliferation of human colon cancer cells
Gene_expression (expression) of MEK2 in colon associated with colon cancer
15) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.66 Pain Relevance 0
Immunohistochemistry analysis of a colorectal cancer tissue microarray containing over 400 colorectal cancer and 50 normal colon tissue biopsies revealed that 44% of colorectal cancers display high cytoplasmic expression of phosphorylated MEK1/MEK2 as compared to 10% of normal tissues (analysis to be published elsewhere).
Gene_expression (expression) of MEK2 in colon associated with colon cancer
16) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.53 Pain Relevance 0
SW480 cells display a comparable expression pattern of MEK1 and MEK2 proteins as HCT116 cells (Fig. 6A).
Gene_expression (expression) of MEK2 in SW480
17) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.37 Pain Relevance 0
Notably, higher levels of MMP-13 protein were detected in IEC-6 cells expressing the activated MEK2 isoform.
Gene_expression (expressing) of MEK2 in IEC-6
18) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.13 Pain Relevance 0.19
We also showed that silencing of MEK1 or MEK2 expression significantly reduces the extent of ERK1 and ERK2 activating phosphorylation (Fig. 7).
Gene_expression (expression) of MEK2
19) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.29 Pain Relevance 0
Overexpression of wild type MEK1 or MEK2 did not affect the expression of endogenous MEK isoforms.
Gene_expression (Overexpression) of MEK2
20) Confidence 0.62 Published 2008 Journal BMC Cancer Section Body Doc Link PMC2596176 Disease Relevance 0.41 Pain Relevance 0

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