INT17275

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Context Info
Confidence 0.75
First Reported 1990
Last Reported 2010
Negated 14
Speculated 4
Reported most in Body
Documents 267
Total Number 271
Disease Relevance 232.07
Pain Relevance 24.06

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (HIVEP1) intracellular (HIVEP1) DNA binding (HIVEP1)
cytoplasm (HIVEP1)
Anatomy Link Frequency
plasma 11
HeLa 9
T cells 9
brain 7
blood 6
HIVEP1 (Homo sapiens)
Pain Link Frequency Relevance Heat
cytokine 427 100.00 Very High Very High Very High
chemokine 336 100.00 Very High Very High Very High
Pain 248 100.00 Very High Very High Very High
cocaine 69 100.00 Very High Very High Very High
opiate 39 100.00 Very High Very High Very High
Central nervous system 845 99.98 Very High Very High Very High
Morphine 51 99.90 Very High Very High Very High
Opioid 56 99.84 Very High Very High Very High
depression 99 99.74 Very High Very High Very High
Lamotrigine 43 99.62 Very High Very High Very High
Disease Link Frequency Relevance Heat
Acquired Immune Deficiency Syndrome Or Hiv Infection 18094 100.00 Very High Very High Very High
Infection 7533 100.00 Very High Very High Very High
Apoptosis 4798 100.00 Very High Very High Very High
Targeted Disruption 428 100.00 Very High Very High Very High
Reprotox - General 2 201 100.00 Very High Very High Very High
Cytomegalovirus Infection 112 100.00 Very High Very High Very High
Hepatitis B Virus Infection 109 100.00 Very High Very High Very High
Syndrome 91 100.00 Very High Very High Very High
Disease Progression 133 99.96 Very High Very High Very High
Sprains And Strains 759 99.92 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Immunoblot analysis demonstrated that expression of GAAPs did not interfere with Bax expression levels (Figure 5B, right panel).
Gene_expression (expression) of GAAP
1) Confidence 0.75 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.91 Pain Relevance 0
Thus, overexpression of human and viral GAAPs provides partial protection against apoptosis triggered by the extrinsic pathway, and this inhibition takes place downstream of caspase-8 cleavage.


Gene_expression (overexpression) of GAAP in cleavage associated with apoptosis
2) Confidence 0.75 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.40 Pain Relevance 0
To examine the kinetics of v-GAAP protein expression, cells were infected with CMLV in the presence or absence of cytosine arabinoside (AraC), an inhibitor of DNA replication and late gene expression.
Spec (examine) Gene_expression (expression) of GAAP protein
3) Confidence 0.75 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.41 Pain Relevance 0
Immunofluorescence using U2OS-h-GAAP cells confirmed that h-GAAP protein expression was reduced to background by 36 h after transfection with GAAP siRNAs 1 and 3, but not with siRNA2 or GFP-siRNA (Figure 4E).
Gene_expression (expression) of GAAP protein
4) Confidence 0.75 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.37 Pain Relevance 0.05
Immunofluorescence analysis showed that 36 h post transfection, GAAP siRNA1 down-regulated h-GAAP and v-GAAP, whereas siRNAs 2 and 3 did not affect v-GAAP expression, and siRNA2 affected neither h-GAAP nor v-GAAP (Figure 7A).
Gene_expression (expression) of v-GAAP
5) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.07 Pain Relevance 0
Accordingly, we tested GAAP siRNAs 1–3 for their ability to down-regulate v-GAAP in the U2OS human cell line that we had engineered to stably express v-GAAP, U2OS-v-GAAP.
Gene_expression (express) of U2OS-v-GAAP
6) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.08 Pain Relevance 0
To investigate whether h-GAAP was expressed in different human tissue, complementary DNA (cDNA) from 16 human tissues obtained from multiple disease-free individuals was used for PCR analysis of h-GAAP gene expression.
Gene_expression (expression) of h-GAAP associated with disease
7) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.25 Pain Relevance 0
These analyses showed that the kinetics of expression of the CMLV and VACV v-GAAP genes were indistinguishable.
Gene_expression (expression) of v-GAAP
8) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.36 Pain Relevance 0
Immunoblotting demonstrated that h-GAAP protein expression was undetectable 56 h after transfection with GAAP siRNAs 1 and 3, but not with siRNA2 and GFP-siRNA (Figure 4F).
Gene_expression (expression) of GAAP protein
9) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.55 Pain Relevance 0.04
Overexpression of h-GAAP or v-GAAP rendered cells resistant to induction of apoptosis, while knock down of expression of the human gene by siRNA caused cells to die by apoptosis.
Gene_expression (Overexpression) of v-GAAP associated with targeted disruption and apoptosis
10) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 1.00 Pain Relevance 0.03
Accordingly, we tested GAAP siRNAs 1–3 for their ability to down-regulate v-GAAP in the U2OS human cell line that we had engineered to stably express v-GAAP, U2OS-v-GAAP.
Gene_expression (express) of v-GAAP
11) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.08 Pain Relevance 0
To investigate whether h-GAAP was expressed in different human tissue, complementary DNA (cDNA) from 16 human tissues obtained from multiple disease-free individuals was used for PCR analysis of h-GAAP gene expression.
Gene_expression (expressed) of h-GAAP associated with disease
12) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.32 Pain Relevance 0
Stable expression of both viral GAAP (v-GAAP) and human GAAP (h-GAAP), which is expressed in all human tissues tested, inhibited apoptosis induced by intrinsic and extrinsic apoptotic stimuli.
Gene_expression (expression) of v-GAAP associated with apoptosis
13) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Abstract Doc Link PMC1803007 Disease Relevance 1.06 Pain Relevance 0
Overexpression of h-GAAP or v-GAAP rendered cells resistant to induction of apoptosis, while knock down of expression of the human gene by siRNA caused cells to die by apoptosis.
Gene_expression (Overexpression) of h-GAAP associated with targeted disruption and apoptosis
14) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 1.00 Pain Relevance 0.03
This showed that h-GAAP is expressed in all human tissue examined (Figure 2C).
Gene_expression (expressed) of h-GAAP
15) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.25 Pain Relevance 0
Consistent with the cell morphology and the result of HeLa cell transfection (Figure 4B), U2OS-h-GAAP cells transfected with GAAP siRNAs 1 and 3 and U2OS-v-GAAP transfected with GAAP siRNA1 showed reduced uptake of TMRE (unpublished data).
Gene_expression (transfected) of U2OS-v-GAAP in HeLa
16) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.24 Pain Relevance 0
In contrast, U2OS-v-GAAP cells, in which endogenous h-GAAP was down-regulated but v-GAAP was still expressed (siRNA3), remained healthy (Figure 7B, upper panels).
Gene_expression (expressed) of v-GAAP in upper
17) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.20 Pain Relevance 0
To examine if GAAP expression was polarised to either the cis or trans Golgi cisternae, U2OS-v-GAAP and U2OS-h-GAAP cells were analysed by immunoelectron microscopy using protein-A gold (Figure 3B) or immunocytochemistry (unpublished data). v-GAAP was expressed throughout the stacks of the Golgi complex (Figure 3B, upper left panel), and similar results were obtained for h-GAAP (unpublished data).
Gene_expression (expressed) of v-GAAP in upper
18) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.14 Pain Relevance 0
In U2OS cells stably expressing h-GAAP or v-GAAP, the expression levels varied among individual cells, but, in all cases, GAAPs co-localised with the Golgi markers GM130 (Figure 3A) and giantin (unpublished data).
Gene_expression (expressing) of h-GAAP
19) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.21 Pain Relevance 0
Just as in HeLa cells (Figure 4B), U2OS-h-GAAP cells transfected with h-GAAP siRNAs 1 and 3, but not siRNA2, died by 48 h post transfection (Figure 7B, lower panels).
Gene_expression (transfected) of with h-GAAP siRNAs 1 and 3 in HeLa
20) Confidence 0.65 Published 2007 Journal PLoS Pathogens Section Body Doc Link PMC1803007 Disease Relevance 0.18 Pain Relevance 0

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