INT173654

Context Info Confidence 0.62 First Reported 2003 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 12 Total Number 12 Disease Relevance 3.16 Pain Relevance 1.43

cytosol (MAPK14) nucleoplasm (MAPK14) signal transduction (MAPK14) nucleus (MAPK14) response to stress (MAPK14) cytoplasm (MAPK14)

Anatomy Link Frequency annulus 2 nucleus 1 vesicle 1 MAPK14 (Homo sapiens)

Pain Link Frequency Relevance Heat Kinase C 6 96.60 cytokine 103 96.32 Inflammation 96 96.28 Eae 2 93.88 Osteoarthritis 314 93.12 imagery 4 82.52 antagonist 27 76.80 chemokine 1 70.72 Immobilon 1 62.84 Central nervous system 45 58.76

Disease Link Frequency Relevance Heat Targeted Disruption 15 97.24 Aging 116 96.48 INFLAMMATION 112 96.28 Osteoarthritis 324 93.12 Amyloid Plaque 2 88.72 Apoptosis 35 88.48 Necrosis 15 87.96 Dementia 2 84.64 Diabetic Retinopathy 2 84.36 Disease 52 81.40

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Primers used for PCR assisted amplification were: p38?

Localization (were) of p38

1) Confidence 0.62 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0 Pain Relevance 0.07

Our analysis showed that mitogen-activated protein kinase p38 (also know as p38, p38 MPA kinase or MPAK14) was significantly upregulated in senescent compared to non-senescent annulus cells.

Localization (kinase) of p38 in annulus

2) Confidence 0.42 Published 2010 Journal BMC Biotechnol Section Body Doc Link PMC2828399 Disease Relevance 0.41 Pain Relevance 0

Our analysis showed that mitogen-activated protein kinase p38 (also know as p38, p38 MPA kinase or MPAK14) was significantly upregulated in senescent compared to non-senescent annulus cells.

Localization (kinase) of p38 in annulus

3) Confidence 0.42 Published 2010 Journal BMC Biotechnol Section Body Doc Link PMC2828399 Disease Relevance 0.41 Pain Relevance 0

At this time (45 min), both p38 kinase and Akt phosphorylated forms were diminished.

Localization (forms) of p38 kinase

4) Confidence 0.37 Published 2005 Journal Arthritis Res Ther Section Body Doc Link PMC1065327 Disease Relevance 0.38 Pain Relevance 0.03

Downstream nuclear events in JNK, p38, and NF-?

Localization (events) of p38

5) Confidence 0.30 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC1906809 Disease Relevance 0 Pain Relevance 0

Finally, clinical pathology results revealed the presence of activated p38?

Localization (presence) of p38

6) Confidence 0.25 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2604896 Disease Relevance 0.39 Pain Relevance 0.25

Consistent with these proposed biological roles in cell function, p38 MAPKs are found in both the nucleus and cytoplasm [14,26].

Localization (found) of p38 in nucleus

7) Confidence 0.24 Published 2008 Journal BMC Neurosci Section Body Doc Link PMC2604896 Disease Relevance 0.19 Pain Relevance 0.36

-N-ethyl-carboxamidoadenosine (NECA) induced interleukin-8 secretion through the involvement of p38 mitogen-activated protein kinase and extracellular signal-regulated protein kinase kinase and this effect was blocked by 3-isobutyl-8-pyrrolidinoxanthine (IPDX) suggesting a basis for the development of new antiasthmatic drugs [24–26].

Localization (secretion) of p38

8) Confidence 0.20 Published 2006 Journal Purinergic Signal Section Body Doc Link PMC2096665 Disease Relevance 0.43 Pain Relevance 0.16

Biochemical analysis and confocal microscopic analysis established that S100A9 translocates to the plasma membrane, secretory vesicle, and gelatinase granules dependently on p38 MAPK-mediated phosphorylation.

Localization (translocates) of p38 in vesicle

9) Confidence 0.13 Published 2008 Journal J Leukoc Biol Section Body Doc Link PMC2567897 Disease Relevance 0 Pain Relevance 0

Indeed, our recent studies have shown that activation of the p38, ERK, and JNK MAP kinases is required for the chondrogenic induction and maintenance of TGF-?

Localization (kinases) of p38

10) Confidence 0.11 Published 2003 Journal Arthritis Res Ther Section Body Doc Link PMC154434 Disease Relevance 0.23 Pain Relevance 0

B, while leptin-induced PGE2 production was reduced by inhibitors of JNK, p38, and Erk1/2 MAPkinases and transcription factor NF-?

Localization (/) of p38

11) Confidence 0.10 Published 2009 Journal Mediators of Inflammation Section Body Doc Link PMC2726438 Disease Relevance 0.46 Pain Relevance 0.43

These findings support the hypothesis that the JNK and p38 MAPKs as well as the NF-?

Localization (support) of p38

12) Confidence 0.07 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656251 Disease Relevance 0.25 Pain Relevance 0.13

General Comments

This test has worked.