INT182435

Context Info Confidence 0.76 First Reported 2005 Last Reported 2008 Negated 0 Speculated 1 Reported most in Body Documents 36 Total Number 37 Disease Relevance 1.66 Pain Relevance 0.58

nucleoplasm (OGG1) mitochondrion (OGG1) lyase activity (OGG1) hydrolase activity, acting on glycosyl bonds (OGG1) nucleolus (OGG1) nucleus (OGG1)

Anatomy Link Frequency HeLa 3 nucleus 3 kidney cortex 1 crescent 1 terminal segment 1 OGG1 (Homo sapiens)

Pain Link Frequency Relevance Heat Glutamate 36 94.48 imagery 36 83.76 Pain 36 51.76 cerebral cortex 1 5.00 transdermal 1 5.00 adenocard 1 5.00 Angina 1 5.00

Disease Link Frequency Relevance Heat Stress 38 93.96 Cancer 344 92.80 Apoptosis 3 90.08 Tuberous Sclerosis 11 87.76 Sprains And Strains 3 62.48 Contagious Ecthyma 36 58.72 Cockayne Syndrome 72 54.24 Renal Cancer 3 52.16 Pain 36 51.76 Disease 57 43.36

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Furthermore, the nucleolar localization of EGFP-hOGG1 is linked to transcription.

Localization (localization) of EGFP-hOGG1

1) Confidence 0.76 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.11 Pain Relevance 0

EGFP/EYFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 localization during S-phase in live cells

Localization (localization) of EGFP-hOGG1

2) Confidence 0.76 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

To compare the localization of EGFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 in living cells during S-phase, stable cell lines were transiently transfected with HcRed-PCNA.

Localization (localization) of EGFP-hOGG1

3) Confidence 0.76 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

EGFP-hOGG1 cells were synchronized at the G1/S boundary, released and allowed to enter S-phase before being treated with 0.04 ?

Localization (released) of EGFP-hOGG1

4) Confidence 0.76 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

EGFP/EYFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 localization during S-phase in live cells

Localization (localization) of EYFP-hOGG1

5) Confidence 0.76 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

As shown in Figure 8B, EGFP-hOGG1-Cys326 is not associated with the nucleoli while the localization of EGFP-hOGG1-Glu326 looks similar to the wild-type protein.

Localization (localization) of EGFP-hOGG1

6) Confidence 0.76 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.03

EGFP/EYFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 localization during S-phase in live cells

Localization (localization) of EYFP-hOGG1

7) Confidence 0.71 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

EGFP/EYFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 localization during S-phase in live cells

Localization (localization) of EGFP-hOGG1

8) Confidence 0.71 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

At this time, EGFP-hOGG1 is mainly distributed evenly throughout the nucleus (Figure 4).

Localization (distributed) of EGFP-hOGG1 in nucleus

9) Confidence 0.71 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

At 2–8 h after nocodazole removal (data not shown), and at the time of double-thymidine block release, the cells are in G1, and EGFP-hOGG1 is mainly homogeneously distributed to the nucleus (Figure 4).

Localization (distributed) of EGFP-hOGG1 in nucleus

10) Confidence 0.71 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Furthermore, during mitosis, from pro-metaphase to telophase, the EGFP-hOGG1-Glu326 signal was co-localized with the condensed chromosomes (Figure 7B shows examples of cells in pro-metaphase and in telophase).

Localization (localized) of EGFP-hOGG1

11) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

Using this approach we showed that EGFP-hOGG1 was relocalized during the cell cycle and it was associated with the nucleoli during S-phase and with condensed chromosomes during mitosis.

Localization (relocalized) of EGFP-hOGG1

12) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.06 Pain Relevance 0

Transient transfection of HeLa cells with EGFP-hOGG1-Ala326 showed the distribution to be identical to that of EGFP-hOGG1-Cys326 indicating that the presence of the serine residue is important for the correct localization of hOGG1 to the nucleoli (data not shown).

Localization (localization) of hOGG1 in HeLa

13) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

The localization of EGFP-hOGG1 to the nucleoli is linked to transcription

Localization (localization) of EGFP-hOGG1

14) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

EGFP/EYFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 localization during S-phase in live cells

Localization (localization) of EGFP-hOGG1

15) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

To compare the localization of EGFP-hOGG1, EGFP-hOGG1-Cys326 and EGFP-hOGG1-Glu326 in living cells during S-phase, stable cell lines were transiently transfected with HcRed-PCNA.

Localization (localization) of EGFP-hOGG1

16) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

We, therefore, used the EGFP tag to study the subcellular localization of hOGG1.

Localization (localization) of hOGG1

17) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.06 Pain Relevance 0

In addition, to confirm the localization of EGFP-hOGG1 to the nucleoli during S-phase, we visualized sites of replication using anti-BrdU antibodies.

Localization (localization) of EGFP-hOGG1

18) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0

Supporting this idea is the restoration of wild-type localization of the EGFP-hOGG1-Glu326 mutant protein that mimicked a phosphorylated serine.

Localization (localization) of EGFP-hOGG1

19) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0.09 Pain Relevance 0

Throughout S-phase, EGFP-hOGG1 is localized around the nucleoli as shown in Figure 8A, which shows a cell during S-phase.

Localization (localized) of EGFP-hOGG1

20) Confidence 0.67 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1072800 Disease Relevance 0 Pain Relevance 0.04

General Comments

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