INT182995

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Context Info
Confidence 0.69
First Reported 2005
Last Reported 2008
Negated 2
Speculated 0
Reported most in Body
Documents 19
Total Number 34
Disease Relevance 13.28
Pain Relevance 8.81

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cytosol (KCNIP3) signal transduction (KCNIP3) Golgi apparatus (KCNIP3)
endoplasmic reticulum (KCNIP3) plasma membrane (KCNIP3) nucleus (KCNIP3)
Anatomy Link Frequency
HeLa 4
nervous system 3
SK-N-MC 2
fibroblast 2
HL-60 1
KCNIP3 (Homo sapiens)
Pain Link Frequency Relevance Heat
Pain 386 99.44 Very High Very High Very High
Osteoarthritis 448 99.08 Very High Very High Very High
Lasting pain 192 98.28 Very High Very High Very High
Dynorphin 160 98.08 Very High Very High Very High
Kappa opioid receptor 128 97.28 Very High Very High Very High
Spinal cord 64 90.84 High High
Opioid 64 88.56 High High
Pain management 48 88.40 High High
potassium channel 16 88.12 High High
Inflammation 16 87.88 High High
Disease Link Frequency Relevance Heat
Neuroblastoma 108 99.58 Very High Very High Very High
Pain 626 99.44 Very High Very High Very High
Repression 152 99.36 Very High Very High Very High
Frailty 464 99.08 Very High Very High Very High
Rheumatoid Arthritis 360 98.24 Very High Very High Very High
Alzheimer's Dementia 16 91.36 High High
INFLAMMATION 16 87.88 High High
Injury 32 87.08 High High
Arthritis 80 75.00 Quite High
Nociception 48 72.40 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Interestingly, in neuroblastoma SK-N-MC cells, which express high DREAM levels (2), overexpression of DREAM failed to increase basal activity of the DR3-TK-CAT reporter (Figure 2B).
Gene_expression (overexpression) of DREAM in SK-N-MC associated with neuroblastoma
1) Confidence 0.69 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.17 Pain Relevance 0
(b) Does targeted inhibition of DREAM expression in synovial fibroblast-like cells (SFLCs) enhance the endogenous level of dynophin action on KOR in the periphery?
Gene_expression (expression) of DREAM in fibroblast associated with kappa opioid receptor
2) Confidence 0.67 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483451 Disease Relevance 0.72 Pain Relevance 0.58
The level of DREAM expression in siRNA-GFP-transfected cells (relative expression 13.78 ± 0.67) served as baseline control and was not statistically different from mock-transfected cells (relative expression 13.65 ± 0.21; mock/siRNA-GFP P = 0.686) (Figure 2).
Gene_expression (expression) of DREAM
3) Confidence 0.67 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483451 Disease Relevance 0.70 Pain Relevance 0.55
The targeted inhibition of the expression of DREAM in SFLCs might enhance the endogenous level of dynorphin acting on KOR, using siRNAs locally in the periphery.
Gene_expression (expression) of DREAM associated with dynorphin and kappa opioid receptor
4) Confidence 0.67 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483451 Disease Relevance 0.90 Pain Relevance 0.63
As shown in Figure 7B, expression of neither native DREAM nor the EF-LCDmut affected significantly the activity of GAL-VDR or GAL-RAR in the absence of ligand.
Gene_expression (expression) of DREAM
5) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.08 Pain Relevance 0
To further investigate a relationship between vitamin D responsiveness and the activation of VDRE-dependent transcription by DREAM, we performed DR3-TK-CAT reporter assays in pituitary and neuroblastoma cells, which in contrast with HeLa cells express DREAM (2,35).
Gene_expression (express) of DREAM in HeLa associated with neuroblastoma
6) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.16 Pain Relevance 0
Overexpression of DREAM in GH4C1 cells caused an increase in basal reporter activity, though less pronounced than that observed in HeLa cells that lack this factor, and significantly enhanced the ligand-dependent response (Figure 2B).
Gene_expression (Overexpression) of DREAM in HeLa
7) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.16 Pain Relevance 0
HeLa cells possess endogenous VDR, but do not express DREAM (2,28).
Neg (not) Gene_expression (express) of DREAM in HeLa
8) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0 Pain Relevance 0
As shown in Figure 2A, expression of DREAM did not significantly affect the activity of the empty TK reporter, confirming previous results (2).
Gene_expression (expression) of DREAM
9) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0 Pain Relevance 0
Future studies will be needed to identify additional genes in DREAM expressing tissues, which contain HRE-like DREAM binding sites and are regulated by this transcription factor.



Gene_expression (expressing) of DREAM
10) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0 Pain Relevance 0
Interestingly, in neuroblastoma SK-N-MC cells, which express high DREAM levels (2), overexpression of DREAM failed to increase basal activity of the DR3-TK-CAT reporter (Figure 2B).
Gene_expression (express) of DREAM in SK-N-MC associated with neuroblastoma
11) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.17 Pain Relevance 0
As occurred with the DR3-containing promoter, DREAM was able to increase the activity of the R90 construct in HeLa and GH4C1 cells, but not in the neuroblastoma cells, that already express high endogenous DREAM levels.
Gene_expression (express) of DREAM in HeLa associated with neuroblastoma
12) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.63 Pain Relevance 0
In addition to expressing DREAM, pituitary somatolactotrop cells contain endogenous VDR and are responsive to vitamin D (27).
Gene_expression (expressing) of DREAM in pituitary
13) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.16 Pain Relevance 0
In addition, binding of in vitro translated DREAM with VDR or RAR fused to GST was analyzed in pull-down assays.
Gene_expression (translated) of DREAM
14) Confidence 0.60 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0.07 Pain Relevance 0
Inhibiting DREAM expression using small interfering RNAs
Gene_expression (expression) of DREAM
15) Confidence 0.59 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483451 Disease Relevance 0.88 Pain Relevance 0.66
In addition, contradicting the theory of DREAM action on pain relief, a reduction of the expression level of DREAM was shown in PBMCs from OA patients with a VAS score of greater than 40 (Figure 2).
Gene_expression (expression) of DREAM associated with pain and osteoarthritis
16) Confidence 0.58 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483451 Disease Relevance 0.70 Pain Relevance 0.84
DREAM expression had a very strong stimulatory effect on HRE-dependent transcription in cells that do not express this factor, but was weaker in cells expressing high endogenous levels of DREAM protein.
Gene_expression (expressing) of DREAM protein
17) Confidence 0.53 Published 2005 Journal Nucleic Acids Research Section Body Doc Link PMC1084319 Disease Relevance 0 Pain Relevance 0
DREAM, also known as calsenilin and KChIP3, is a member of the recoverin/neuronal calcium sensor family of nuclear calcium-binding proteins and so far has mainly been known to be expressed in the nervous system [29-31].
Gene_expression (expressed) of calsenilin in nervous system
18) Confidence 0.52 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2483451 Disease Relevance 0.25 Pain Relevance 0.09
To our knowledge, this is the first report showing the expression of DREAM in SFLCs and PBMCs on the mRNA level.
Gene_expression (expression) of DREAM
19) Confidence 0.52 Published 2008 Journal Arthritis Res Ther Section Abstract Doc Link PMC2483451 Disease Relevance 1.04 Pain Relevance 0.71
However, DREAM protein was not detectable.
Neg (not) Gene_expression (detectable) of DREAM protein
20) Confidence 0.52 Published 2008 Journal Arthritis Res Ther Section Abstract Doc Link PMC2483451 Disease Relevance 0.97 Pain Relevance 0.67

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