INT191739

Context Info Confidence 0.49 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 6 Total Number 6 Disease Relevance 0.76 Pain Relevance 0.04

16S (Mus musculus)

Pain Link Frequency Relevance Heat Inflammation 13 71.88 spastic colon 28 18.72 Crohn's disease 26 5.00 cINOD 9 5.00 Pain 3 5.00 Inflammatory response 2 5.00 antagonist 2 5.00 adenocard 1 5.00 spinal inflammation 1 5.00 abdominal pain 1 5.00

Disease Link Frequency Relevance Heat Bacterial Respiratory Disease 10 97.60 Acute Disease 2 96.80 Gastritis 40 95.32 Infection 40 91.40 Disease 109 79.00 INFLAMMATION 9 71.88 Periodontal Pocket 4 62.48 Diarrhoea 52 50.00 Skin Infection 6 50.00 Clostridium Difficile Infection 4 50.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The full 16S rRNA gene was sequenced using the MicroSeq Full Gene 16S rRNA Bacterial Isolation Sequencing Kit (Applied Biosystems, Foster City, California, United States), according to the manufacturer's protocol, and sequences were analyzed using the 3100 Genetic Analyzer (Applied Biosystems).

Positive_regulation (sequenced) of 16S

1) Confidence 0.49 Published 2006 Journal PLoS Pathogens Section Body Doc Link PMC1435791 Disease Relevance 0 Pain Relevance 0

numerical analysis of the V3-16S rRNA gene fingerprints representing the

Positive_regulation (fingerprints) of 16S

2) Confidence 0.49 Published 2008 Journal Interdisciplinary Perspectives on Infectious Diseases Section Body Doc Link PMC2648627 Disease Relevance 0.26 Pain Relevance 0.04

sequencing of certain loci, such as the highly conserved 16S rRNA locus, are

Positive_regulation (conserved) of 16S

3) Confidence 0.49 Published 2008 Journal Interdisciplinary Perspectives on Infectious Diseases Section Body Doc Link PMC2649424 Disease Relevance 0 Pain Relevance 0

approach discussed above (16S rRNA gene sequencing) to 7 patients with initial (n = 3) and recurrent (n = 4) CDAD and 3 control individuals from an outpatient

Positive_regulation (sequencing) of 16S

4) Confidence 0.49 Published 2008 Journal Interdisciplinary Perspectives on Infectious Diseases Section Body Doc Link PMC2649424 Disease Relevance 0.27 Pain Relevance 0

Colonies were sequenced for the 16S rRNA gene for identification.

Positive_regulation (sequenced) of 16S

5) Confidence 0.49 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2776972 Disease Relevance 0.16 Pain Relevance 0

Interestingly the same samples containing the novel mcrA sequence types also revealed 16S rRNA gene sequences grouping within the Thermoplasmatales but with no cultured relatives.

Positive_regulation (sequences) of 16S

6) Confidence 0.49 Published 2010 Journal Archaea Section Body Doc Link PMC3021867 Disease Relevance 0.06 Pain Relevance 0

General Comments

This test has worked.