INT208915

Context Info Confidence 0.29 First Reported 2007 Last Reported 2008 Negated 0 Speculated 1 Reported most in Body Documents 3 Total Number 5 Disease Relevance 1.02 Pain Relevance 0.25

cell differentiation (Nkx2-5) nucleus (Nkx2-5) DNA binding (Nkx2-5) transcription factor binding (Nkx2-5) cytoplasm (Nkx2-5)

Anatomy Link Frequency J82 1 skeletal muscle 1 bladder 1 Nkx2-5 (Mus musculus)

Pain Link Frequency Relevance Heat substance P 66 99.52 Inflammation 84 58.68 Serotonin 3 23.44 antagonist 15 5.00 Inflammatory response 12 5.00 Inflammatory mediators 6 5.00 Pain 6 5.00 interstitial cystitis 6 5.00 Kinase C 4 5.00 Lasting pain 3 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 22 98.60 Toxicity 4 97.36 Muscular Dystrophy 2 92.00 Disease 32 90.08 Cancer 12 66.60 Hypopituitarism 22 60.64 INFLAMMATION 99 58.68 Myotonic Dystrophy 16 50.00 Bladder Disease 9 39.12 Natriuresis 6 35.16

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Results show that instillation of SP into the mouse bladder increased the amount of shifted Nkx-2.5 and NF-kappaB probes that peaked at 24 hours post stimulation (Figure 9 A-D).

Positive_regulation (increased) of Nkx-2.5 in bladder associated with substance p

1) Confidence 0.29 Published 2007 Journal BMC Urol Section Body Doc Link PMC1888709 Disease Relevance 0 Pain Relevance 0.09

To determine whether activation of NK1R increases the transcriptional activities of NF-kappaB and Nkx-2.5, an EMSA was performed.

Spec (whether) Positive_regulation (increases) of Nkx-2.5

2) Confidence 0.21 Published 2007 Journal BMC Urol Section Body Doc Link PMC1888709 Disease Relevance 0 Pain Relevance 0.10

We, therefore, generated preliminary results using an urothelial cell line (J82) which indicated a constitutive activation of both NF-kappaB and NKx-2.5 in the absence of overt stimulation (data not shown).

Positive_regulation (activation) of NKx-2.5 in J82

3) Confidence 0.20 Published 2007 Journal BMC Urol Section Body Doc Link PMC1888709 Disease Relevance 0.18 Pain Relevance 0.06

Moreover, overexpression of DMPK 3' UTR mRNA in mouse skeletal muscle also induced transcriptional activation of Nkx2-5 and its targets.

Positive_regulation (activation) of Nkx2-5 in skeletal muscle

4) Confidence 0.02 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0.42 Pain Relevance 0

Levels of NKX2-5 increased in cardiac tissue in a reversible transgenic mouse model for RNA toxicity [43].

Positive_regulation (increased) of NKX2-5 associated with targeted disruption and toxicity

5) Confidence 0.01 Published 2008 Journal Current Genomics Section Body Doc Link PMC2694559 Disease Relevance 0.42 Pain Relevance 0

General Comments

This test has worked.