INT211906

Context Info Confidence 0.19 First Reported 2007 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 15 Total Number 17 Disease Relevance 3.75 Pain Relevance 0.61

nucleoplasm (EPC1) nucleus (EPC1)

Anatomy Link Frequency circulating cells 2 monocytes 2 endothelial cells 2 blood 1 hematopoietic stem cell 1 EPC1 (Homo sapiens)

Pain Link Frequency Relevance Heat cva 22 94.84 Inflammation 70 86.84 cytokine 16 85.12 metalloproteinase 9 81.44 addiction 25 75.40 Angina 9 67.32 opiate 9 50.00 Potency 4 39.72 Pain 3 19.12 alcohol 11 5.00

Disease Link Frequency Relevance Heat Drug Dependence 31 100.00 Necrosis 7 99.92 Myocardial Infarction 76 97.80 Cv General 3 Under Development 31 94.84 Coronary Artery Disease 91 92.32 Disease 55 89.12 Acute Coronary Syndrome 9 88.36 Cardiovascular Disease 13 87.44 INFLAMMATION 74 86.84 Increased Venous Pressure Under Development 17 83.64

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Significant increases of circulating cells expressing the EPC phenotype were observed at days 2 (36.12% compared to pre-treatment control p = 0.04) and 7 (95.35% compared to pretreatment control, p = .001) as shown in Figure 5.

Gene_expression (expressing) of EPC in circulating cells

1) Confidence 0.19 Published 2010 Journal J Transl Med Section Body Doc Link PMC2862021 Disease Relevance 0 Pain Relevance 0.04

Stem-Kine augments circulation of cells with EPC phenotype

Gene_expression (phenotype) of EPC in Stem

2) Confidence 0.17 Published 2010 Journal J Transl Med Section Body Doc Link PMC2862021 Disease Relevance 0 Pain Relevance 0.04

Stem-Kine increases circulating cells with EPC activity

Gene_expression (activity) of EPC in circulating cells

3) Confidence 0.17 Published 2010 Journal J Transl Med Section Body Doc Link PMC2862021 Disease Relevance 0 Pain Relevance 0.04

According to our previous transcript profiling, three highly expressed transcripts for each EPC type were chosen; HLA-DRA, lysozyme, and CD14 for eEPCs, and caveolin1, VE-cadherin, and vWF for OECs.

Gene_expression (expressed) of EPC

4) Confidence 0.16 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0.14 Pain Relevance 0.07

CB-derived as opposed to PB-derived eEPCs and OECs were also included in transcriptomic analysis as it has been suggested that foetal blood may represent a richer EPC source than adult peripheral blood [23,24].

Gene_expression (source) of EPC in blood

5) Confidence 0.16 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0 Pain Relevance 0

An alternative approach to isolate EPCs from peripheral or umbilical cord blood utilizes in vitro culture and this consistently produces two distinct EPC subtypes which have been named as early EPCs (eEPCs) and Outgrowth endothelial cells (OECs) [11].

Gene_expression (produces) of EPC in OECs

6) Confidence 0.16 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0.48 Pain Relevance 0.07

Furthermore, eEPC gene expression patterns partially matched monocytes, and OECs were closely linked to DMECs.

Gene_expression (expression) of eEPC in monocytes

7) Confidence 0.16 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0 Pain Relevance 0.04

In conclusion, the current study suggests feasibility of significant mobilization of cells expressing hematopoietic stem cell and EPC markers and properties.

Gene_expression (expressing) of EPC in hematopoietic stem cell

8) Confidence 0.15 Published 2010 Journal J Transl Med Section Body Doc Link PMC2862021 Disease Relevance 0.16 Pain Relevance 0

Heat maps generated using GenePattern software for the top 30 distinctly expressed transcripts in each EPC subtype show expression patterns at the gene level consistent with hierarchical cluster analysis (Figure 4B, C).

Gene_expression (expression) of EPC

9) Confidence 0.14 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0 Pain Relevance 0.03

Therefore, to elucidate and differentiate the expressed phenotypes of endothelial progenitors, eEPC and OEC transcriptomes were directly compared with monocytes and endothelial cells.

Gene_expression (expressed) of eEPC in endothelial cells

10) Confidence 0.14 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0 Pain Relevance 0

Using a single component (PC1) it was possible to separate samples into two groups; one containing the three eEPC biological replicates with a high PC1 value, and other group with the three OEC biological replicates showing a low PC1 value (Figure 1D).

Gene_expression (value) of eEPC

11) Confidence 0.14 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0.10 Pain Relevance 0

Heat maps generated using GenePattern software for the top 30 distinctly expressed transcripts in each EPC subtype show expression patterns at the gene level consistent with hierarchical cluster analysis (Figure 4B, C).

Gene_expression (expressed) of EPC

12) Confidence 0.14 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0 Pain Relevance 0.03

Currently, the combinations of CD133+CD34+KDR+, CD34+KDR+, or CD14+CD34low [9], [10], [11], are widely used to define or select cells that express properties attributed to EPC.

Gene_expression (express) of EPC

13) Confidence 0.10 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2972200 Disease Relevance 0.07 Pain Relevance 0

Nevertheless, there are ongoing debates about the definition of ‘true’ EPC, as well as the availability of a reliable method to assess their quantity and quality, functional status, and therapeutic application.

Gene_expression (definition) of EPC

14) Confidence 0.10 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2972200 Disease Relevance 0.33 Pain Relevance 0

Patients with ACS were further stratified for troponin T positivity to account for potential effects of myocardial necrosis on EPC levels.

Gene_expression (levels) of EPC associated with necrosis and myocardial infarction

15) Confidence 0.10 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2972200 Disease Relevance 1.87 Pain Relevance 0.22

An approximate F statistic approached significance (P = 0.07), indicating weak evidence in favour of a different relationship between age and both HSC and EPC for the SUD and N-SUD groups – with the SUD group having lower numbers of both HSC's and EPC's compared with NA group members of equivalent age.

Gene_expression (groups) of EPC associated with drug dependence

16) Confidence 0.07 Published 2007 Journal Subst Abuse Treat Prev Policy Section Body Doc Link PMC1949819 Disease Relevance 0.60 Pain Relevance 0.04

Therefore, to elucidate and differentiate the expressed phenotypes of endothelial progenitors, eEPC and OEC transcriptomes were directly compared with monocytes and endothelial cells.

Gene_expression (expressed) of eEPC in monocytes

17) Confidence 0.05 Published 2010 Journal BMC Med Genomics Section Body Doc Link PMC2881111 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.