INT217742

Context Info Confidence 0.67 First Reported 2007 Last Reported 2010 Negated 1 Speculated 1 Reported most in Body Documents 20 Total Number 21 Disease Relevance 9.76 Pain Relevance 4.23

extracellular space (COL2A1) extracellular region (COL2A1) cytoplasm (COL2A1)

Anatomy Link Frequency chondrocyte 12 cartilage 8 endothelial cells 2 cleavage 2 upper 2 COL2A1 (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 545 100.00 metalloproteinase 108 99.56 Inflammation 161 99.08 cytokine 82 98.84 Arthritis 80 95.24 nociceptor 3 81.20 Paracetamol 24 80.12 fibrosis 7 78.80 Pain 124 76.56 cINOD 24 74.64

Disease Link Frequency Relevance Heat Osteoarthritis 528 100.00 Hypertrophy 75 100.00 INFLAMMATION 178 99.08 Disease 107 98.56 Apoptosis 152 98.28 Arthritis 108 95.24 Leukemia 5 94.16 Aging 12 84.04 Knee Osteoarthritis 43 83.72 Frailty 58 82.56

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

It did not upregulate chondrocyte proliferation and type II collagen synthesis as evidenced by the lack of significant changes in the expression of cyclin B2 and COL2A1, respectively.

Neg (not) Positive_regulation (upregulate) of Gene_expression (synthesis) of type II collagen in chondrocyte

1) Confidence 0.67 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2206385 Disease Relevance 0.53 Pain Relevance 0.16

generation [33], to stimulate collagen and proteoglycan synthesis [11-13] and chondrocyte proliferation [11], and to inhibit chondrocyte terminal differentiation/hypertrophy [24,25] and apoptosis in endothelial cells [34].

Positive_regulation (stimulate) of Gene_expression (synthesis) of collagen in endothelial cells associated with hypertrophy and apoptosis

2) Confidence 0.45 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2206385 Disease Relevance 0.86 Pain Relevance 0.24

This involves phenotypic hypertrophy-related changes in chondrocytes, such as the production of type X collagen (COL10A1) (hypertrophy marker), and the upregulation of collagenase matrix metalloproteinase (MMP)-13, as is seen in the fetal growth plate [1-3].

Positive_regulation (upregulation) of Gene_expression (production) of collagen in growth plate associated with hypertrophy and metalloproteinase

3) Confidence 0.42 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2206385 Disease Relevance 0.92 Pain Relevance 0.61

In the present article we report that physiological tonicity (380 mOsm) during isolation and monolayer expansion can suppress chondrocyte dedifferentiation and that expression of the extracelluar matrix components collagen type I and collagen type II as well as aggrecan is NFAT5 dependent.

Positive_regulation (dependent) of Gene_expression (expression) of collagen type II in chondrocyte

4) Confidence 0.41 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2911888 Disease Relevance 0.47 Pain Relevance 0.15

Aigner and colleagues have suggested that the expression of COL2A1 is suppressed in the upper zones of early OA cartilage, but increased in late-stage OA cartilage relative to normal controls [31], [32].

Positive_regulation (increased) of Gene_expression (expression) of COL2A1 in upper associated with osteoarthritis

5) Confidence 0.41 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3012157 Disease Relevance 1.24 Pain Relevance 0.55

Controversy exists among others about whether COL2A1 expression is increased or suppressed in OA cartilage.

Spec (whether) Positive_regulation (increased) of Spec (whether) Gene_expression (expression) of COL2A1 in cartilage associated with osteoarthritis

6) Confidence 0.41 Published 2010 Journal PLoS ONE Section Body Doc Link PMC3012157 Disease Relevance 1.23 Pain Relevance 0.55

They used adenoviral delivery vectors expressing Sox9 to infect a chondroblastic cell line and human disc cells; Sox9 and Type II collagen production increased.

Positive_regulation (increased) of Gene_expression (production) of Type II collagen

7) Confidence 0.32 Published 2008 Journal International Journal of Biological Sciences Section Body Doc Link PMC2532796 Disease Relevance 0.36 Pain Relevance 0

However, there were small, but significant, increases in gene expression for COL2A1 and ACAN in AC cells when compared with NP cells (P = 0.011 and 0.026, respectively; Figure 2).

Positive_regulation (increases) of Gene_expression (expression) of COL2A1

8) Confidence 0.23 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875656 Disease Relevance 0.12 Pain Relevance 0

Aggrecan and collagen type-II gene expression, which is characteristic for hyaline cartilage, stayed elevated, while expression of the collagen type-I gene, indicating dedifferentiated chondrocytes, was slowly decreasing during cultivation.

Positive_regulation (elevated) of Gene_expression (expression) of collagen type-II gene in chondrocytes associated with osteoarthritis

9) Confidence 0.21 Published 2010 Journal BMC Complement Altern Med Section Body Doc Link PMC2921357 Disease Relevance 0.18 Pain Relevance 0

Gene expression of collagen type-II, collagen type-I, aggrecan, MMP-13, IL-6, Il-1?

Positive_regulation (Gene) of Gene_expression (expression) of collagen type-II

10) Confidence 0.15 Published 2010 Journal BMC Complement Altern Med Section Body Doc Link PMC2921357 Disease Relevance 0.11 Pain Relevance 0

In one of these clinical studies, progression was identified by the increase in type II collagen cleavage products compared to a decrease in the propeptide marker of synthesis [2].

Positive_regulation (increase) of Gene_expression (products) of type II collagen in cleavage

11) Confidence 0.13 Published 2007 Journal Arthritis Res Ther Section Body Doc Link PMC2212578 Disease Relevance 0.48 Pain Relevance 0.23

Studies using explants of human cartilage indicate that catabolic degradation of cartilage matrix, a MMP dependent response, is suppressed by reparagen in concert with increased expression of the cartilage repair factor, IGF-1 [20].

Positive_regulation (increased) of Gene_expression (expression) of cartilage repair factor in cartilage

12) Confidence 0.12 Published 2007 Journal BMC Complement Altern Med Section Body Doc Link PMC2131759 Disease Relevance 0.05 Pain Relevance 0.14

Collagen type II expression in MPCs was low and found to be only increased in the presence of 10 and 20 ?

Positive_regulation (increased) of Gene_expression (expression) of Collagen type II

13) Confidence 0.11 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875662 Disease Relevance 0.06 Pain Relevance 0

For the 7-day and 10-day culture periods, a progressive stimulation of collagen type II synthesis was observed with PPS concentrations up to 5 ?

Positive_regulation (stimulation) of Gene_expression (synthesis) of collagen type II

14) Confidence 0.11 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875662 Disease Relevance 0 Pain Relevance 0

Reparagen is derived from South American medicinal plants and supported by preclinical studies demonstrating chondroprotective, cytoprotective, and anti-inflammatory properties [21-24] as well as the ability to enhance human chondrocyte production of the cartilage repair factor, IGF-1 [20].

Positive_regulation (enhance) of Gene_expression (production) of cartilage repair factor in cartilage associated with inflammation

15) Confidence 0.08 Published 2007 Journal BMC Complement Altern Med Section Body Doc Link PMC2131759 Disease Relevance 0.28 Pain Relevance 0.29

The aforementioned findings were consistent with the real-time PCR results, which showed the corresponding upregulation of SOX-9, aggrecan and type II collagen gene expression but downregulation of type I collagen expression by MPCs cultured in the presence of PPS.

Positive_regulation (upregulation) of Gene_expression (expression) of type II collagen gene

16) Confidence 0.08 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875662 Disease Relevance 0.09 Pain Relevance 0

In the 5-day cultures, lower but significant (P < 0.001 to 0.05) stimulation of collagen type II synthesis by MPCs was still evident at PPS concentrations up to 20 ?

Positive_regulation (stimulation) of Gene_expression (synthesis) of collagen type II

17) Confidence 0.08 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2875662 Disease Relevance 0 Pain Relevance 0

synthesis, and enhances type II collagen and aggrecan core protein synthesis in human articular chondrocytes [24].

Positive_regulation (enhances) of Gene_expression (synthesis) of type II collagen in chondrocytes

18) Confidence 0.05 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2888220 Disease Relevance 0.36 Pain Relevance 0.15

For instance, treatment with PGD2 enhances the expression of the cartilage-specific matrix components collagen type II and aggrecan [7] and prevents chondrocyte apoptosis [8].

Positive_regulation (enhances) of Gene_expression (expression) of collagen type II in chondrocyte associated with apoptosis

19) Confidence 0.03 Published 2008 Journal Arthritis Res Ther Section Body Doc Link PMC2656251 Disease Relevance 0.59 Pain Relevance 0.28

It stimulates collagen type II and proteoglycan expression, inhibits MMP, proinflammatory cytokine or nitric oxide expression and protects against chondrocyte apoptosis (reviewed in [71]).

Positive_regulation (stimulates) of Gene_expression (expression) of collagen type II in chondrocyte associated with metalloproteinase, apoptosis and cytokine

20) Confidence 0.02 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2911903 Disease Relevance 1.31 Pain Relevance 0.67

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