INT223459

Context Info Confidence 0.71 First Reported 2007 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 2 Total Number 8 Disease Relevance 1.50 Pain Relevance 0.16

cytosol (Dock4) cytoplasm (Dock4)

Anatomy Link Frequency eyes 1 photoreceptor 1 finger 1 Dock4 (Mus musculus)

Pain Link Frequency Relevance Heat Trk A 1 91.28 nociceptor 18 58.76 Glutamate receptor 2 55.80 Nerve growth factor 8 48.44 Neuropeptide 3 31.84 sodium channel 1 29.76 anesthesia 14 5.00 GABAergic 7 5.00 Pain 5 5.00 Spinal cord 3 5.00

Disease Link Frequency Relevance Heat Osteogenic Sarcomas 7 100.00 Myopia 91 98.68 Adhesions 7 98.36 Death 14 97.76 Ganglion Cysts 16 89.48 Usher Syndrome 7 88.72 Syndrome 8 86.00 Nonsyndromic Deafness 7 85.04 Targeted Disruption 8 84.48 Blindness 7 82.16

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

However, the differential expression of Dock4 (p=0.07) and Myb (p=0.09) just missed significance, and the differential expression of Usp36 and Zmym4 also could not be confirmed.

Gene_expression (expression) of Dock4

1) Confidence 0.71 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0.08 Pain Relevance 0

Krt2-6b, Myb, and Dock4 expression

Gene_expression (expression) of Dock4

2) Confidence 0.71 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0.33 Pain Relevance 0

Accordingly, eight potentially interesting genes were selected for validation: early growth response 1 (Egr-1), protein kinase B (Akt2), keratin complex 2, basic, gene 6b (Krt2-6b), myeloblastosis oncogene (Myb), FBJ osteosarcoma oncogene (cFos), ubiquitin specific peptidase 36 (Usp36), zinc finger, MYM-type 4 (Zmym4), and dedicator of cytokinesis 4 (Dock4).

Gene_expression (selected) of Dock4 in finger associated with osteogenic sarcomas

3) Confidence 0.62 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0.10 Pain Relevance 0

Dock4 is a potent rac activator and an unconventional guanine exchange factor for the Rho family of guanosine triphosphatases (Rho GEF GTPases), as a protein interacting with harmonin, which is expressed in the inner and outer photoreceptor segments.

Gene_expression (expressed) of Dock4 in photoreceptor

4) Confidence 0.62 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0.86 Pain Relevance 0

Baseline expression levels of Akt2, Krt2-6b, Myb, Zmym4, Usp36, and Dock4

Gene_expression (levels) of Dock4

5) Confidence 0.55 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0 Pain Relevance 0

The mRNA expression of Akt2 and Dock4 increased in both diffuser- and ND filter-treated eyes, however, the increase was more pronounced in the diffuser-treated eyes.

Gene_expression (expression) of Dock4 in eyes

6) Confidence 0.55 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0 Pain Relevance 0

Baseline expression levels of Akt2, Krt2-6b, Myb, Zmym4, Usp36, and Dock4

Gene_expression (expression) of Dock4

7) Confidence 0.55 Published 2007 Journal Molecular Vision Section Body Doc Link PMC2774458 Disease Relevance 0 Pain Relevance 0

When expressed as "fold-change in expression" the SAGE results demonstrate a down regulation of Grik1, Dock4 and Crip2 expression between P0 WT and P0 Trka-/- DRG of about 18, 6 and 3 fold respectively, while QRT-PCR provided an expression ratio between P0 WT and P0 Trka-/- DRG of 17, 4 and 4 fold respectively.

Gene_expression (expression) of Dock4

8) Confidence 0.53 Published 2007 Journal BMC Neurosci Section Body Doc Link PMC2241628 Disease Relevance 0.13 Pain Relevance 0.16

General Comments

This test has worked.