INT226668

Context Info Confidence 0.29 First Reported 2008 Last Reported 2008 Negated 0 Speculated 1 Reported most in Body Documents 1 Total Number 5 Disease Relevance 2.77 Pain Relevance 0.13

endosome (Cxcr4) signal transduction (Cxcr4) cytoplasmic membrane-bounded vesicle (Cxcr4) plasma membrane (Cxcr4) signal transducer activity (Cxcr4)

Anatomy Link Frequency T cells 2 Cxcr4 (Mus musculus)

Pain Link Frequency Relevance Heat Kinase C 20 66.56 imagery 10 5.00 chemokine 5 5.00 Inflammatory response 5 5.00 Opioid 5 5.00

Disease Link Frequency Relevance Heat Acquired Immune Deficiency Syndrome Or Hiv Infection 375 98.64 Infection 50 94.00 Apoptosis 20 91.60 Cytomegalovirus Infection 5 87.64 Superinfection 20 50.68 Cancer 15 5.00 INFLAMMATION 5 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Incubation of Gag-GFP expressing Jurkat cells with SDF-1, PMA and ionomycin revealed that downregulation of endogenous CXCR4 was clearly attenuated by expression of wt Gag-GFP (Fig. 3C,D,E).

Positive_regulation (attenuated) of Negative_regulation (downregulation) of CXCR4

1) Confidence 0.29 Published 2008 Journal Virol J Section Body Doc Link PMC2262066 Disease Relevance 0.82 Pain Relevance 0

In order to determine whether SDF-1 induced HA-CXCR4 downregulation is dependent on the ESCRT-I complex, cells were depleted of the critical ESCRT-I component, TSG101.

Spec (whether) Positive_regulation (dependent) of Spec (whether) Negative_regulation (downregulation) of CXCR4

2) Confidence 0.29 Published 2008 Journal Virol J Section Body Doc Link PMC2262066 Disease Relevance 0 Pain Relevance 0

SDF-1 induced CXCR4 downregulation is ESCRT-I dependent

Positive_regulation (induced) of Negative_regulation (downregulation) of CXCR4

3) Confidence 0.27 Published 2008 Journal Virol J Section Body Doc Link PMC2262066 Disease Relevance 0.34 Pain Relevance 0

We speculate that during the late stages of the viral life cycle when mostly structural proteins such as Gag are expressed, SDF-1 induced CXCR4 downregulation is attenuated resulting in the accumulation of densensitized CXCR4 within intracellular compartments.

Positive_regulation (induced) of Negative_regulation (downregulation) of CXCR4

4) Confidence 0.27 Published 2008 Journal Virol J Section Body Doc Link PMC2262066 Disease Relevance 0.74 Pain Relevance 0.13

In order to examine the effects of Gag expression on endogenous CXCR4, we monitored the kinetics of SDF-1 induced downregulation of CXCR4 in Jurkat T cells.

Positive_regulation (induced) of Negative_regulation (downregulation) of CXCR4 in T cells

5) Confidence 0.27 Published 2008 Journal Virol J Section Body Doc Link PMC2262066 Disease Relevance 0.86 Pain Relevance 0

General Comments

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