INT236167

Context Info Confidence 0.06 First Reported 2007 Last Reported 2007 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 3 Disease Relevance 0.18 Pain Relevance 0.07

molecular_function (Lins) cellular_component (Lins) biological_process (Lins)

Anatomy Link Frequency brain 1 heads 1 Lins (Mus musculus)

Pain Link Frequency Relevance Heat anesthesia 3 74.08 imagery 63 14.24 Peripheral nervous system 3 13.64 Spinal cord 48 5.00 medulla 6 5.00 unmyelinated 6 5.00 cytokine 3 5.00 chemokine 3 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 9 100.00 Dislocations 3 82.80 Death 3 40.44 Apoptosis 3 5.00 Spinal Cord Compression 3 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

The systematic use of GFP, while extremely useful for the analysis of single gene mutations or wildtype brain development, actually slows down mutant analysis because GFP expressing transgenic lines (Lichtman and Conchello, 2005; Shaner et al., 2005) have to be crossed into the mutant lines, which then have to be bred back to homozygosity.

Gene_expression (expressing) of lines in brain associated with targeted disruption

1) Confidence 0.06 Published 2007 Journal Immunological Investigations Section Body Doc Link PMC2430174 Disease Relevance 0.10 Pain Relevance 0

For this study we used E18.5/P0 mouse embryonic spinal cords or heads of wild-type or several mutant lines with or without expression of GFP.

Gene_expression (expression) of lines in heads

2) Confidence 0.05 Published 2007 Journal Immunological Investigations Section Body Doc Link PMC2430174 Disease Relevance 0.08 Pain Relevance 0.07

Unfortunately, the conditional approach is not always feasible, since widespread expression of genes used for the currently available Cre lines to eliminate the LoxP flanked gene(s) of choice often gives rise to adverse effects in organs not targeted (Ohyama and Groves, 2004).

Gene_expression (expression) of lines

3) Confidence 0.04 Published 2007 Journal Immunological Investigations Section Body Doc Link PMC2430174 Disease Relevance 0 Pain Relevance 0

General Comments

This test has worked.