INT242532

Context Info Confidence 0.50 First Reported 2008 Last Reported 2010 Negated 2 Speculated 1 Reported most in Body Documents 14 Total Number 14 Disease Relevance 2.38 Pain Relevance 1.44

mitochondrion (Chat) transferase activity, transferring acyl groups (Chat)

Anatomy Link Frequency neurons 16 dorsal horn 2 nucleus ambiguus 2 Chat (Mus musculus)

Pain Link Frequency Relevance Heat Spinal cord 73 99.84 Action potential 198 99.78 Dorsal horn 2 96.60 medulla 18 95.68 agonist 7 94.56 Inflammation 29 94.24 substance P 22 71.48 Nerve growth factor 59 60.28 Root ganglion neuron 3 56.36 Neuropeptide 14 31.56

Disease Link Frequency Relevance Heat Urological Neuroanatomy 48 99.84 Stress 17 98.88 Disease 139 97.04 INFLAMMATION 33 94.24 Insulin Resistance 2 90.64 Ganglion Cysts 137 88.60 Targeted Disruption 68 86.24 Diabetes Mellitus 10 83.96 Death 24 77.92 Injury 3 75.76

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Somatic size was similar for ChAT+ and ChAT?

Positive_regulation (for) of Gene_expression (similar) of ChAT

1) Confidence 0.50 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0

During action potential trains at 10 Hz, the normalized action potential amplitude (normalized to the first action potential) was 0.85±0.03 for ChAT+ neurons and 0.92±0.02 for ChAT?

Positive_regulation (0.03) of Gene_expression (neurons) of ChAT in neurons associated with action potential

2) Confidence 0.43 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0.15

neurons (ChAT+ neurons were 35.3×15.8 µm; ChAT?

Positive_regulation (neurons) of Gene_expression (15.8) of ChAT in neurons

3) Confidence 0.43 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0

During action potential trains at 10 Hz, the normalized action potential amplitude (normalized to the first action potential) was 0.85±0.03 for ChAT+ neurons and 0.92±0.02 for ChAT?

Positive_regulation (for) of Gene_expression (neurons) of ChAT in neurons associated with action potential

4) Confidence 0.43 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0.15

During action potential trains at 10 Hz, the normalized action potential amplitude (normalized to the first action potential) was 0.85±0.03 for ChAT+ neurons and 0.92±0.02 for ChAT?

Positive_regulation (for) of Gene_expression (neurons) of ChAT in neurons associated with action potential

5) Confidence 0.43 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0.15

Somatic size was similar for ChAT+ and ChAT?

Positive_regulation (for) of Gene_expression (similar) of ChAT

6) Confidence 0.43 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0

With increasing current injections the spiking rates of ChAT+ neurons increased to a plateau frequency of ?

Positive_regulation (increased) of Gene_expression (neurons) of ChAT in neurons

7) Confidence 0.43 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2883570 Disease Relevance 0 Pain Relevance 0.05

We determined whether the SCCs express ChAT and VAChT, two critical elements for synthesis and packaging of ACh, a potential transmitter.

Spec (whether) Positive_regulation (packaging) of Spec (whether) Gene_expression (express) of ChAT

8) Confidence 0.34 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2912856 Disease Relevance 0.08 Pain Relevance 0.07

The overlay of both channels is shown in c. d–f Magnification of the region of the nucleus ambiguus identified by high levels of ChAT expression (e).

Positive_regulation (levels) of Gene_expression (expression) of ChAT in nucleus ambiguus

9) Confidence 0.29 Published 2009 Journal Pflugers Arch Section Body Doc Link PMC2691554 Disease Relevance 0 Pain Relevance 0.12

The data show that ret is required for the development of cholinergic sympathetic neurons at advanced developmental stages but not for the induction of ChAT and VAChT expression early during embryonic development.

Neg (not) Positive_regulation (induction) of Gene_expression (expression) of ChAT in neurons

10) Confidence 0.26 Published 2008 Journal Cell Tissue Res Section Body Doc Link PMC2516536 Disease Relevance 0.30 Pain Relevance 0

After the ret-independent initial induction of ChAT and VAChT expression in the majority or even all sympathetic neurons, the cholinergic markers are lost in most cells and become expressed at comparatively high levels in a small subset of sympathetic neurons (Fig. 5).

Neg (independent) Positive_regulation (induction) of Gene_expression (expression) of ChAT in neurons

11) Confidence 0.26 Published 2008 Journal Cell Tissue Res Section Body Doc Link PMC2516536 Disease Relevance 0 Pain Relevance 0.06

In the DMV, 1.5 and 3 months rotenone treatment resulted in increased intracellular alpha-synuclein accumulation inside ChAT+ neurons (Figure 3B) when compared to controls (Figure 3A).

Positive_regulation (accumulation) of Gene_expression (neurons) of ChAT in neurons

12) Confidence 0.23 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808242 Disease Relevance 0.40 Pain Relevance 0.20

We observed an increase in alpha-synuclein and some large alpha-synuclein intracellular inclusions (2% of the analyzed images) in ChAT+ neurons of IML (>7.5 µm) (Figure 2A–C) as well as increased alpha-synuclein staining in the dorsal horn layer I after 1.5 and 3 months treatment (Figure 2G and 2H) when compared to controls.

Positive_regulation (increase) of Gene_expression (neurons) of ChAT in dorsal horn associated with dorsal horn and spinal cord

13) Confidence 0.23 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2808242 Disease Relevance 0.67 Pain Relevance 0.39

phosphorylation, and augmenting choline acetyltransferase (ChAT) expression (de la Monte et al., 2006).

Positive_regulation (augmenting) of Gene_expression (expression) of choline acetyltransferase

14) Confidence 0.10 Published 2010 Journal Frontiers in Aging Neuroscience Section Body Doc Link PMC2912024 Disease Relevance 0.92 Pain Relevance 0.10

General Comments

This test has worked.