INT244779

Context Info Confidence 0.47 First Reported 2008 Last Reported 2008 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 9 Disease Relevance 0.14 Pain Relevance 0.03

cell differentiation (Lbx1) nucleus (Lbx1) DNA binding (Lbx1)

Anatomy Link Frequency nodes 3 Lbx1 (Mus musculus)

Pain Link Frequency Relevance Heat Spinal cord 18 57.56 Pain 9 37.92 substantia gelatinosa 18 15.12 Central nervous system 9 5.72 Dorsal horn neuron 9 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 54 76.12 Pain 9 37.92 Apoptosis 18 5.00 Acquired Immune Deficiency Syndrome Or Hiv Infection 9 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

There is also a clear interlocking of the two data sets because 30 of the known nodes are Lbx1 targets and because virtually all of the known Lbx1 interactions were recapitulated in the ANCEA dataset.

Lbx1 Binding (interactions) of in nodes

1) Confidence 0.47 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0

We demonstrated this by comparing green (Lbx1+) and white (Lbx1?)

Lbx1 Binding (white) of

2) Confidence 0.36 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0

Primer pairs for Lbx1, activated targets (Pax2, Lmx1b, Pax8, Pax2?

Lbx1 Binding (pairs) of

3) Confidence 0.36 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0.03

For example, in this report, we compared gene expression of heterozygote Lbx1GFP/+ green cells with null Lbx1GFP/GFP green cells to discover epistatic interactions between Lbx1 and target SSTFs.

Lbx1 Binding (interactions) of

4) Confidence 0.36 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0.07 Pain Relevance 0

We demonstrated this by comparing green (Lbx1+) and white (Lbx1?)

Lbx1 Binding (green) of

5) Confidence 0.36 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0

The interaction between Lbx1 and a high fraction of the available SSTF nodes, the strong association of Lbx1 target SSTFs with the densest UCR clusters, and the ability to observe combinatorial codes by immunohistochemistry are inconsistent with standard hierarchical models of cell lineage specification, in which “master regulators” at the top of the lineage control hierarchies of lineage-specific SSTFs.

Lbx1 Binding (interaction) of in nodes

6) Confidence 0.35 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0

Constraining epistasis analysis of Lbx1 to only those cells that normally express Lbx1 allowed unprecedented sensitivity in identifying Lbx1 network interactions and allowed the interactions to be assigned to a specific set of cell populations.

Lbx1 Binding (interactions) of

7) Confidence 0.35 Published 2008 Journal PLoS ONE Section Abstract Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0

Either Lbx1 is a highly connected hub in some or all of the five populations that are currently defined, or there are far more populations in which Lbx1 participates in only a few of interactions we discovered.

Lbx1 Binding (interactions) of

8) Confidence 0.35 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0.08 Pain Relevance 0

The interaction between Lbx1 and a high fraction of the available SSTF nodes, the strong association of Lbx1 target SSTFs with the densest UCR clusters, and the ability to observe combinatorial codes by immunohistochemistry are inconsistent with standard hierarchical models of cell lineage specification, in which “master regulators” at the top of the lineage control hierarchies of lineage-specific SSTFs.

Lbx1 Binding (association) of in nodes

9) Confidence 0.35 Published 2008 Journal PLoS ONE Section Body Doc Link PMC2527684 Disease Relevance 0 Pain Relevance 0

General Comments

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