INT245023

Context Info Confidence 0.58 First Reported 2008 Last Reported 2008 Negated 0 Speculated 1 Reported most in Body Documents 1 Total Number 5 Disease Relevance 0.90 Pain Relevance 0

nucleus (Xrcc1) intracellular (Xrcc1)

Anatomy Link Frequency SH-SY5Y 1 Xrcc1 (Mus musculus)

Pain Link Frequency Relevance Heat bDMF 5 5.00 anesthesia 5 5.00

Disease Link Frequency Relevance Heat Infection 20 93.16 Neuroblastoma 20 87.08 Neurodegenerative Disease 15 83.04 Hypersensitivity 15 79.04 Spinocerebellar Ataxia Type 2 15 77.68 Rheumatoid Arthritis 55 77.32 Apoptosis 80 69.36 Ataxia 25 27.92 Neuropathic Pain 10 21.76 Stress 85 11.76

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Stable suppression of the XRCC1 protein was achieved using the targeting shRNA system, while the scramble-shRNA infected cells retained XRCC1 expression.

Negative_regulation (suppression) of XRCC1

1) Confidence 0.58 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2528184 Disease Relevance 0.16 Pain Relevance 0

XRCC1 is a key component of SSBR, as cells defective in this nonenzymatic scaffold protein show reduced SSBR, enhanced sensitivity to DNA-damaging agents that produce SSBs, and increased sister chromatid exchange (5).

Negative_regulation (defective) of XRCC1

2) Confidence 0.43 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2528184 Disease Relevance 0 Pain Relevance 0

To determine the functional consequence of XRCC1 down-regulation, we evaluated methyl methanesulfonate (MMS) sensitivity.

Spec (determine) Negative_regulation (regulation) of XRCC1

3) Confidence 0.43 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2528184 Disease Relevance 0.16 Pain Relevance 0

Using hypoxanthine–xanthine oxidase as the oxidizing method, XRCC1 deficiency affected both dividing and nondividing SH-SY5Y cells, with a greater effect on survival seen in the former case, suggesting that the spectrum of oxidative DNA damage created dictates the specific contribution of XRCC1 to cellular resistance.

Negative_regulation (deficiency) of XRCC1 in SH-SY5Y

4) Confidence 0.43 Published 2008 Journal Nucleic Acids Research Section Abstract Doc Link PMC2528184 Disease Relevance 0.32 Pain Relevance 0

Shown is a representative western blot of XRCC1 protein expression in dividing XKD and control cells. ?

Negative_regulation (blot) of XRCC1

5) Confidence 0.42 Published 2008 Journal Nucleic Acids Research Section Body Doc Link PMC2528184 Disease Relevance 0.26 Pain Relevance 0

General Comments

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