INT264717

Context Info Confidence 0.42 First Reported 2008 Last Reported 2009 Negated 0 Speculated 0 Reported most in Body Documents 7 Total Number 7 Disease Relevance 0.77 Pain Relevance 0.03

cell adhesion (Hes1) nucleus (Hes1) intracellular (Hes1) protein complex assembly (Hes1) DNA binding (Hes1) transcription factor binding (Hes1)

Anatomy Link Frequency Daoy 1 Hes1 (Mus musculus)

Pain Link Frequency Relevance Heat Central nervous system 6 59.40 imagery 76 5.00 ketamine 7 5.00 anesthesia 7 5.00 alcohol 6 5.00

Disease Link Frequency Relevance Heat Repression 6 100.00 Cancer 450 93.88 Apoptosis 30 72.24 Severe Combined Immunodeficiency 6 64.08 Central Nervous System Cancer 6 59.64 Hepatocellular Cancer 6 46.84 Bladder Cancer 6 41.44 Lung Cancer 12 38.48 Medulloblastoma 42 5.00 Brain Tumor 18 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

These effects of miR-199b-5p on HES1 protein expression were not restricted to the stable clones or Daoy cells, as D283MED cells transiently transfected with the expression construct for miR-199b-5p also showed reduced HES1 levels (Text S1, Fig.

Negative_regulation (reduced) of HES1 in Daoy

1) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.08 Pain Relevance 0

Three clones demonstrated reduced HES1 protein levels, one of which (199bSC1) showed no detectable HES1.

Negative_regulation (reduced) of HES1

2) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0 Pain Relevance 0

Here, HES1 levels were restored, suggesting 2-OM block of HES1 repression by miR-199b-5p, providing further confirmation that miR-199b-5p targets HES1 directly.

Negative_regulation (repression) of HES1 associated with repression

3) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.10 Pain Relevance 0

We believe these findings to be due to an overall lower efficiency of 199bMC1 for reducing HES1 protein levels.

Negative_regulation (reducing) of HES1

4) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.06 Pain Relevance 0

Here, HES1 levels were restored, suggesting 2-OM block of HES1 repression by miR-199b-5p, providing further confirmation that miR-199b-5p targets HES1 directly.

Negative_regulation (repression) of HES1 associated with repression

5) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.10 Pain Relevance 0

To confirm that the cellular phenotype is directly correlated with down-regulation of HES1, we performed in vitro cell-rescue experiments.

Negative_regulation (regulation) of HES1

6) Confidence 0.42 Published 2009 Journal PLoS ONE Section Body Doc Link PMC2656623 Disease Relevance 0.43 Pain Relevance 0.03

Analysis of RNA synthesis in RPCs using RT-PCR revealed marked down-regulation of Pax6, Hes1, B3-tubulin, DCX and partial down-regulation of nestin and Sox2 (Fig. 4).

Negative_regulation (down-regulation) of Hes1

7) Confidence 0.27 Published 2008 Journal J Ocul Biol Dis Infor Section Body Doc Link PMC2802414 Disease Relevance 0 Pain Relevance 0

General Comments

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