INT275604

Context Info Confidence 0.03 First Reported 2006 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 2 Total Number 7 Disease Relevance 1.62 Pain Relevance 0.06

small molecule metabolic process (PLB1) plasma membrane (PLB1)

Anatomy Link Frequency PLB-985 5 granulocytes 1 respiratory 1 PLB1 (Homo sapiens)

Pain Link Frequency Relevance Heat imagery 12 71.88 tolerance 13 56.56 Pain 8 5.00 alcohol 6 5.00 depression 3 5.00 Central nervous system 1 5.00 Spinal cord 1 5.00 antidepressant 1 5.00 medulla 1 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 78 100.00 Dyspnea 48 99.24 Pulmonary Disease 35 97.24 Leukemia 48 91.72 Chronic Lymphoid Leukemia 6 72.16 Muscle Weakness 2 49.60 Pain 8 5.00 Emphysema 8 5.00 Lymphatic System Cancer 6 5.00 Apoptosis 6 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Compared with spontaneous breathing, PLB reduces respiratory rate, dyspnea and arterial carbon dioxide tension, and improves tidal volume and arterial oxygenation at rest (Bianchi et al 2004).

Gene_expression (reduces) of PLB in respiratory associated with dyspnea

1) Confidence 0.03 Published 2006 Journal International Journal of Chronic Obstructive Pulmonary Disease Section Body Doc Link PMC2707811 Disease Relevance 0.57 Pain Relevance 0.03

If the superoxide production of a differentiated PLB-985 clone in which Hv1 expression is knocked-down is also limited by shortage of compensatory charge flow, we should be able to compensate for it using an artificial conductance.

Gene_expression (production) of PLB-985 in PLB-985

2) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2990768 Disease Relevance 0.19 Pain Relevance 0

To test the latter possibility, PLB-985 cell clones that express normal or diminished amounts of Hv1 were differentiated for 7 days in DMFA containing, low-serum medium.

Gene_expression (express) of PLB-985 in PLB-985

3) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2990768 Disease Relevance 0.07 Pain Relevance 0

It is also demonstrated that a massive reduction in Hv1 expression can limit the Nox2 mediated superoxide production of PLB-985 granulocytes.

Gene_expression (production) of PLB-985 in granulocytes

4) Confidence 0.01 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2990768 Disease Relevance 0.11 Pain Relevance 0.04

Surprisingly, however, in pilot experiments the PMA-induced superoxide production of the Hv1 knock-down F2 and B7 PLB-985 clones exceeded that of G9 and G11 control clones, and was only inferior to that of clone E9 (not shown).

Gene_expression (production) of PLB-985 in PLB-985 associated with targeted disruption

5) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2990768 Disease Relevance 0.19 Pain Relevance 0

We suspected that this result might be explained by the large heterogeneity in the Nox2 expression level of PLB-985 clones (Fig. 6b).

Gene_expression (expression) of PLB-985 in PLB-985

6) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2990768 Disease Relevance 0.17 Pain Relevance 0

After the analysis of Hv1 protein expression in puromycin-resistant PLB-985 clones, three control and two Hv1 knock-down clones were tested in whole-cell patch-clamp experiments (Fig. 5a).

Gene_expression (expression) of PLB-985 in PLB-985 associated with targeted disruption

7) Confidence 0.01 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2990768 Disease Relevance 0.32 Pain Relevance 0

General Comments

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