INT277003

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Context Info
Confidence 0.47
First Reported 2009
Last Reported 2009
Negated 1
Speculated 0
Reported most in Body
Documents 1
Total Number 10
Disease Relevance 3.05
Pain Relevance 0.07

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

cell differentiation (MUSK) plasma membrane (MUSK)
Anatomy Link Frequency
plasma 4
muscle 1
band 1
MUSK (Homo sapiens)
Pain Link Frequency Relevance Heat
Multiple sclerosis 10 71.92 Quite High
tolerance 10 69.84 Quite High
Calcium channel 20 20.96 Low Low
anesthesia 10 19.40 Low Low
rheumatoid arthritis 10 5.00 Very Low Very Low Very Low
Inflammation 10 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Myasthenia Gravis 270 100.00 Very High Very High Very High
Disease 190 99.56 Very High Very High Very High
Malignant Neoplastic Disease 20 81.88 Quite High
Hypersensitivity 20 81.28 Quite High
Demyelinating Disease 10 71.92 Quite High
Diabetes Mellitus 20 61.36 Quite High
Autoimmune Disease 50 47.64 Quite Low
Syndrome 10 43.12 Quite Low
Cancer 60 42.40 Quite Low
Neurological Disease 20 35.80 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
Having found that all three MuSK-MG plasmas bound to and immunoprecipitated a biotinylated 90-kDa protein, we wished to estimate the quantity of immunoprecipitated protein upon which mass spectrometry identification was to be attempted.
MuSK-MG Binding (bound) of in plasmas associated with myasthenia gravis
1) Confidence 0.47 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.39 Pain Relevance 0
Another subgroup of myasthenia gravis patients has antibodies to a muscle-specific tyrosine kinase (MuSK).1 These antibodies are known to bind to the cell surface and to inhibit the clustering function of MuSK (10).
MuSK Neg (inhibit) Binding (bind) of in muscle associated with myasthenia gravis
2) Confidence 0.47 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.78 Pain Relevance 0
The three MuSK-MG patients' sera bound to the cells at levels higher than the mean + 2 S.D. of the healthy control sera (Fig. 2a).
MuSK-MG Binding (bound) of associated with myasthenia gravis
3) Confidence 0.41 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.18 Pain Relevance 0
l of MuSK-MG plasma (with a maximum MuSK binding capacity of 62 ng) immunoprecipitated ?
MuSK Binding (binding) of in plasma associated with myasthenia gravis
4) Confidence 0.36 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.16 Pain Relevance 0
This preliminary step demonstrated that an IgG antibody in the MuSK-positive plasmas bound to the TE671 cells and that these cells should provide a good starting point for the identification of the antigenic target(s).


MuSK Binding (bound) of in plasmas
5) Confidence 0.36 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.23 Pain Relevance 0
After SDS-PAGE and Western blotting, the unlabeled cell immunoprecipitate was probed with a goat antibody to MuSK, which bound to the same 90-kDa band believed to be MuSK (Fig. 2d).


MuSK Binding (bound) of in band
6) Confidence 0.36 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.39 Pain Relevance 0
However, these immunoprecipitates contained an excess of IgG that was not bound to MuSK but that bound to the protein G-Sepharose, making it very difficult to identify the antigen in subsequent mass spectrometry experiments.
MuSK Binding (bound) of
7) Confidence 0.36 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.31 Pain Relevance 0
l of MuSK-MG plasma (with a maximum MuSK binding capacity of 62 ng) immunoprecipitated ?
MuSK Binding (capacity) of in plasma associated with myasthenia gravis
8) Confidence 0.36 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.16 Pain Relevance 0
Like many pathogenic disease-specific antibodies, MuSK antibodies bind with high affinity to their target MuSK (12).
MuSK Binding (bind) of associated with disease
9) Confidence 0.35 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.37 Pain Relevance 0.04
To establish this method, we used a model system involving the TE671 cell line and MuSK antibodies that are known to bind to MuSK on the TE671 cells.
MuSK Binding (bind) of
10) Confidence 0.35 Published 2009 Journal Molecular & Cellular Proteomics : MCP Section Body Doc Link PMC2716719 Disease Relevance 0.08 Pain Relevance 0.03

General Comments

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