INT289724

Context Info Confidence 0.55 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 14 Disease Relevance 2.29 Pain Relevance 0.61

intracellular (Sat1) transferase activity, transferring acyl groups (Sat1) cytoplasm (Sat1)

Anatomy Link Frequency hair 1 Sat1 (Mus musculus)

Pain Link Frequency Relevance Heat antagonist 154 96.92 fibrosis 14 64.80 Dismenorea 14 61.60 endometriosis 14 60.40 Kinase C 154 5.00 adenocard 28 5.00 imagery 14 5.00

Disease Link Frequency Relevance Heat Targeted Disruption 42 99.74 Alopecia 14 98.20 Female Infertility 14 97.76 Pancreatitis 14 94.72 Intestinal Cancer 28 79.36 Cancer 14 68.16 Fibrosis 14 64.80 Reprotox - General 1 28 61.60 Endometriosis (extended) 84 60.40

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Protein

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Event

Anatomy

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Speculation

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Disease term

Butaprost-mediated SAT1 gene expression is potentiated by G?

Gene_expression (expression) of SAT1 gene

1) Confidence 0.55 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0

We investigated the integrative signaling mediating the role of prostanoids on SAT1 expression in FPEP2 cells.

Gene_expression (expression) of SAT1

2) Confidence 0.55 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0.38 Pain Relevance 0.03

For example SAT1 gene expression was enhanced from a 5.9 fold increase to a 7.5 fold increase, whereas cytochrome P450, family 26, subfamily A, polypeptide 1 (CYP26A1) gene expression was repressed from an 18.0 fold increase to a 12.3 fold increase.

Gene_expression (expression) of SAT1 gene

3) Confidence 0.55 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0

No significant elevation of SAT1 gene expression was observed following PGF treatment alone.

Gene_expression (expression) of SAT1 gene

4) Confidence 0.55 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.04

q cross-talk via AC3, such that siRNA knockdown of the AC3 isoform completely inhibited the potentiation of Butaprost-stimulated SAT1 expression by PGF.

Gene_expression (expression) of SAT1

5) Confidence 0.48 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0.69 Pain Relevance 0.03

However, co-stimulation of FPEP2 cells with Butaprost and PGF enhanced the Butaprost-stimulated expression of SAT1 significantly at all time points (P < 0.001).

Gene_expression (expression) of SAT1

6) Confidence 0.48 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.04

To determine whether the PGF-mediated potentiation of SAT1 expression is mediated by the FP receptor, the cells were treated with the FP receptor antagonist (AL8810) in the presence/absence of Butaprost and/or PGF for 6 h.

Gene_expression (expression) of SAT1 associated with antagonist

7) Confidence 0.48 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.05

As shown in Fig. 4A, Butaprost treatment alone significantly increased expression of SAT1 at all time points compared to vehicle treatment (P < 0.001).

Gene_expression (expression) of SAT1

8) Confidence 0.48 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.03

As shown in Fig. 4C, ablation of AC3 expression reduced SAT1 mRNA expression in FPEP2 cells treated with the combination of Butaprost and PGF to the level observed following stimulation with Butaprost alone (P < 0.001).

Gene_expression (expression) of SAT1 mRNA

9) Confidence 0.43 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0.24 Pain Relevance 0.13

A transgenic increase of SAT1 expression in mice showed a variety of defects such as hair loss, female infertility, impaired lipid metabolism and predisposition to develop pancreatitis [36].

Gene_expression (expression) of SAT1 in hair associated with targeted disruption, pancreatitis, female infertility and alopecia

10) Confidence 0.43 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0.77 Pain Relevance 0

As shown in Fig. 4B, antagonism of the FP receptor completely abolished the potentiation of SAT1 expression by PGF without altering Butaprost-stimulated expression of SAT1.

Gene_expression (expression) of SAT1

11) Confidence 0.43 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.05

As shown in Fig. 4B, antagonism of the FP receptor completely abolished the potentiation of SAT1 expression by PGF without altering Butaprost-stimulated expression of SAT1.

Gene_expression (expression) of SAT1

12) Confidence 0.43 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.05

q cross-talk in FPEP2 cells, we assessed if the same isoform is involved in PGF-mediated potentiation of SAT1 expression.

Gene_expression (expression) of SAT1

13) Confidence 0.43 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0.21 Pain Relevance 0.12

These data demonstrate that Butaprost-regulated expression of SAT1 is augmented by PGF-FP receptor coupling.

Gene_expression (expression) of SAT1

14) Confidence 0.43 Published 2010 Journal Cell Signal Section Body Doc Link PMC2791881 Disease Relevance 0 Pain Relevance 0.04

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