INT303343

Context Info Confidence 0.65 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 5 Disease Relevance 0.12 Pain Relevance 0.10

cytoplasm (Myh3)

Anatomy Link Frequency muscle tissue 1 thick filaments 1 Myh3 (Mus musculus)

Pain Link Frequency Relevance Heat imagery 10 74.04 cytokine 5 58.24 Spinal cord 5 5.00 Pain 5 5.00 Sciatic nerve 5 5.00

Disease Link Frequency Relevance Heat Muscular Atrophy 45 67.16 Targeted Disruption 5 55.84 Hypertrophy 5 41.12 Frailty 20 33.76 Appetite Loss 5 12.80 Stress 5 11.60 Pain 5 5.00 Sprains And Strains 5 5.00 Injury 5 5.00 Nutritional Deficiencies 5 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

We then separated the P and S myosin preparations on gel filtration columns and assessed the levels and distribution of MyHCemb and Ozz on immunoblots of the eluted fractions probed with anti MyHCemb, anti-Ozz and anti-MLC antibodies.

Gene_expression (antibodies) of MyHCemb

1) Confidence 0.65 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844429 Disease Relevance 0 Pain Relevance 0

We showed that Ozz and Elo C are present together with sarcomeric myosin from the earliest stages of myofibrillogenesis, but we saw no obvious increase in Ozz/MyHCemb ratio in association with the onset of peak periods of MyHCemb degradation during embryogenesis (e.g.

Gene_expression (ratio) of MyHCemb

2) Confidence 0.65 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844429 Disease Relevance 0 Pain Relevance 0

Immunofluorescence analyses of MyHCemb expression in muscle tissue were performed on hind limb muscles from wild-type and ozz?

Gene_expression (expression) of MyHCemb in muscle tissue

3) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844429 Disease Relevance 0 Pain Relevance 0.07

MyHCemb expression is associated with initiation of sarcomere formation [14], leading to the idea that it is optimized for self-assembly into new thick filaments followed by a sequence of subunit changes to give rise to adult myofilaments [15].

Gene_expression (expression) of MyHCemb in thick filaments

4) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844429 Disease Relevance 0.07 Pain Relevance 0.03

To test if expression of MyHCemb protein was also disturbed in the ozz?

Gene_expression (expression) of MyHCemb

5) Confidence 0.58 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2844429 Disease Relevance 0.06 Pain Relevance 0

General Comments

This test has worked.