INT319157

Context Info Confidence 0.56 First Reported 2010 Last Reported 2010 Negated 0 Speculated 3 Reported most in Body Documents 1 Total Number 16 Disease Relevance 0.78 Pain Relevance 0

cell proliferation (Kat2a) nucleus (Kat2a)

Anatomy Link Frequency embryos 6 oocytes 6 Kat2a (Mus musculus)

Pain Link Frequency Relevance Heat anesthesia 16 5.00

Disease Link Frequency Relevance Heat Polycystic Ovary Syndrome 48 91.00 Ovarian Hyperstimulation Syndrome 32 89.32 Dislocations 32 19.44 Cancer 16 5.00 Reprotox - General 3 16 5.00 Immunotherapy Of Cancer 16 5.00 Peripheral Arterial Disease 16 5.00

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

No differences were detected in the expression of GCN5 and HDAC1 after two-cell stage embryos, which indicated that the insufficiency induced by IVM might be rectified during the process of growing from two-cell to four-cell stage.

Gene_expression (expression) of GCN5 in embryos

1) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

We determined the expression and distribution of GCN5, HDAC1, and Ac-H3 in metaphase II oocytes matured in vitro and in vivo.

Spec (determined) Gene_expression (expression) of GCN5 in oocytes

2) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

However, GCN5 protein expressions were decreased in IVM oocytes and two-cell embryos.

Gene_expression (expressions) of GCN5 in oocytes

3) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

Although IVM could affect the expression and distribution of GCN5 and HDAC1 in MII oocytes and 2-cell embryos, which might be one reason for the low BR after 2-cell transfer, the levels of acetyl-histone H3 were not significantly changed in IVM group.

Gene_expression (expression) of GCN5 in embryos

4) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

Meanwhile, in the 2-cell embryos of the control group, GCN5 expression was obviously around karyotheca while in the same stage of embryo from IVM, the distribution of the fluorescent staining of GCN5 was almost even.

Gene_expression (expression) of GCN5 in embryo

5) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

Fluorescence immunocytochemistry showed that GCN5 was expressed in oocytes and embryos in both IVM and control groups.

Gene_expression (expressed) of GCN5 in oocytes

6) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

In conclusion, our studies showed that IVM could affect the expressions of GCN5 and HDAC1 in MII oocytes and two-cell embryos.

Gene_expression (expressions) of GCN5 in embryos

7) Confidence 0.56 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

Histone acetyltransferase GCN5 (GCN5), a type-A HAT, catalyzes the acetylation of nucleosomes in nuclei or free histones in cytoplasm and acts as transcriptional coactivator in gene regulation.

Gene_expression (acetyltransferase) of GCN5

8) Confidence 0.48 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

The precise mechanisms by which IVM affect, the GCN5 and HDAC1 levels and the implication of these changes for the safety of offspring need to be investigated in future studies.

Gene_expression (levels) of GCN5

9) Confidence 0.48 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

The seeming inconsistency between GCN5/HDAC1 levels and H3 acetylation levels is partially caused by protein localization, as IVM-induced changes of GCN5 were observed in the cytoplasm, while Ac-H3 is associated with chromatins that localize in nuclei.

Gene_expression (levels) of GCN5

10) Confidence 0.48 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

The decreased expression and altered distribution of GCN5 in IVM oocytes and two-cell embryos suggested that IVM could disturb the function of GCN5 during the period of reprogramming, which might lead to epigenetic alterations.

Gene_expression (expression) of GCN5 in oocytes

11) Confidence 0.48 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

Results showed that IVM downregulated the protein expression of GCN5 in MII oocytes and two-cell embryos and changed the distribution of GCN5 in two-cell embryos.

Gene_expression (expression) of GCN5 in embryos

12) Confidence 0.43 Published 2010 Journal Obstetrics and Gynecology International Section Abstract Doc Link PMC2896857 Disease Relevance 0.23 Pain Relevance 0

To investigate the influences of IVM on epigenetic reprogramming and to search for the possible reasons for the lower rates of fertilization and cleavage in IVM oocytes, we examined the expression of two enzymes controlling histone acetylation, histone acetyltransferase GCN5 (GCN5) and histone deacetylase 1 (HDAC1), as well as their common target, acetyl-histone H3 (Ac-H3), in mouse metaphase II (MII) oocytes and preimplantation embryos.

Spec (examined) Gene_expression (expression) of GCN5 in oocytes

13) Confidence 0.43 Published 2010 Journal Obstetrics and Gynecology International Section Abstract Doc Link PMC2896857 Disease Relevance 0.27 Pain Relevance 0

Histone acetyltransferase GCN5 (GCN5), a type-A HAT, catalyzes the acetylation of nucleosomes in nuclei or free histones in cytoplasm and acts as transcriptional coactivator in gene regulation.

Gene_expression (acetyltransferase) of GCN5

14) Confidence 0.42 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

To investigate the influences of IVM on epigenetic reprogramming and to search for the possible reasons for the lower rates of fertilization and cleavage in IVM oocytes, we examined the expression of two enzymes controlling histone acetylation, histone acetyltransferase GCN5 (GCN5) and histone deacetylase 1 (HDAC1), as well as their common target, acetyl-histone H3 (Ac-H3), in mouse metaphase II (MII) oocytes and preimplantation embryos.

Spec (examined) Gene_expression (expression) of GCN5 in oocytes

15) Confidence 0.38 Published 2010 Journal Obstetrics and Gynecology International Section Abstract Doc Link PMC2896857 Disease Relevance 0.27 Pain Relevance 0

Fluorescence immunocytochemistry showed that GCN5 was expressed in oocytes and embryos in both IVM and control groups.

Gene_expression (expressed) of GCN5 in embryos

16) Confidence 0.19 Published 2010 Journal Obstetrics and Gynecology International Section Body Doc Link PMC2896857 Disease Relevance 0 Pain Relevance 0

General Comments

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