INT321528

Context Info Confidence 0.41 First Reported 2010 Last Reported 2010 Negated 0 Speculated 1 Reported most in Body Documents 1 Total Number 4 Disease Relevance 0.21 Pain Relevance 0.04

transport (Abcb1b) mitochondrion (Abcb1b) ATPase activity (Abcb1b) transmembrane transport (Abcb1b)

Abcb1b (Mus musculus)

Pain Link Frequency Relevance Heat tolerance 4 77.76 anesthesia 4 5.00 imagery 4 5.00

Disease Link Frequency Relevance Heat Malaria 32 76.00 Recurrence 4 32.00 Sprains And Strains 8 5.00 Cancer 8 5.00 Dislocations 4 5.00 Falciparum Malaria 4 5.00 Disease 4 5.00 Vomiting 4 5.00 Infection 4 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

In order to determine whether the increase in genomic copies and transcription of the mdr1 gene was accompanied by an increase in its product, we measured the relative amount of MDR1 protein in the different parasite clones, using quantitative Western blot analysis.

Spec (whether) Positive_regulation (increase) of Transcription (transcription) of mdr1

1) Confidence 0.41 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0 Pain Relevance 0

Thus, the fold-increase of AS-ATNMF1 mdr1 mRNA transcripts was 2.6±0.17 and 3.4±0.22, relative to its single copy number progenitor AS-ATN and to AS-3CQ, respectively.

Positive_regulation (increase) of Transcription (transcripts) of mdr1

2) Confidence 0.38 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0 Pain Relevance 0

In this context, we investigated by real time PCR whether the evolution of ATN + MF-resistance had selected parasites with increased mdr1 copy number or mRNA expression.

Positive_regulation (increased) of Transcription (expression) of mdr1

3) Confidence 0.38 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904698 Disease Relevance 0 Pain Relevance 0.04

Investigation of candidate genes revealed an mdr1 duplication in the resistant parasites and increased levels of mdr1 transcripts and protein.

Positive_regulation (increased) of Transcription (transcripts) of mdr1

4) Confidence 0.38 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2904698 Disease Relevance 0.21 Pain Relevance 0

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