INT321548

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Context Info
Confidence 0.21
First Reported 2010
Last Reported 2010
Negated 0
Speculated 0
Reported most in Body
Documents 7
Total Number 21
Disease Relevance 8.92
Pain Relevance 0

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

peptidase activity (Ctsb) mitochondrion (Ctsb) extracellular space (Ctsb)
extracellular region (Ctsb) lysosome (Ctsb) cytoplasm (Ctsb)
Anatomy Link Frequency
extracellular matrix 1
Ctsb (Mus musculus)
Pain Link Frequency Relevance Heat
cINOD 15 36.36 Quite Low
metalloproteinase 24 5.00 Very Low Very Low Very Low
potassium channel 6 5.00 Very Low Very Low Very Low
Central nervous system 6 5.00 Very Low Very Low Very Low
Inward rectifier potassium channel 6 5.00 Very Low Very Low Very Low
anesthesia 6 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Glioma 588 99.84 Very High Very High Very High
Apoptosis 147 99.20 Very High Very High Very High
Cancer 753 98.84 Very High Very High Very High
Adhesions 198 98.44 Very High Very High Very High
Colon Cancer 30 97.04 Very High Very High Very High
Metastasis 48 96.68 Very High Very High Very High
Hyperplasia 30 89.36 High High
Leukemia 15 72.08 Quite High
Skin Cancer 15 67.04 Quite High
Lung Cancer 15 56.96 Quite High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
MMP-9, uPAR and cathepsin B downregulation significantly inhibits xenograft cell adhesion to several extracellular matrix proteins.
Negative_regulation (downregulation) of cathepsin B in extracellular matrix associated with adhesions
1) Confidence 0.21 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2904700 Disease Relevance 0.57 Pain Relevance 0
As expected, inhibition of adhesion of these cells to ECM components is much more significant with the simultaneous downregulation of MMP-9 and uPAR or MMP-9 and cathepsin B.
Negative_regulation (downregulation) of cathepsin B associated with adhesions
2) Confidence 0.21 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904700 Disease Relevance 1.43 Pain Relevance 0
In the present study, MMP-9, uPAR and cathepsin B genes were downregulated in glioma xenograft cells using shRNA plasmid constructs and we evaluated the involvement of integrins and changes in their adhesion, migration and invasive potential.


Negative_regulation (downregulated) of cathepsin B associated with adhesions and glioma
3) Confidence 0.21 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2904700 Disease Relevance 0.62 Pain Relevance 0
Reduction in the mRNA levels of MMP-9, uPAR and cathepsin B in M-sh, U-sh and C-sh transfected 4910 cells indicated a downregulation of MMP-9, uPAR and cathespin B target mRNAs, the mechanism by which shRNAs are proposed to work [24], [25].
Negative_regulation (Reduction) of cathepsin B
4) Confidence 0.15 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904700 Disease Relevance 0.18 Pain Relevance 0
The mRNA levels of several alpha integrins were reduced with MMP-9, uPAR and cathepsin B downregulation (Fig. 2B). ?
Negative_regulation (downregulation) of cathepsin B
5) Confidence 0.15 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2904700 Disease Relevance 0.05 Pain Relevance 0
Thus, our findings provide molecular mechanism for the G0/G1 arrest induced by the downregulation of cathepsin B and uPAR in SNB19 and U251 glioma cells.


Negative_regulation (downregulation) of cathepsin B associated with glioma
6) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.39 Pain Relevance 0
Similarly, the present study demonstrates that the simultaneous downregulation of cathepsin B and uPAR caused the regression of intracranial tumors.
Negative_regulation (downregulation) of cathepsin B associated with cancer
7) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.76 Pain Relevance 0
The effect of RNAi-mediated inhibition of cathepsin B and uPAR on pre-established tumors was studied.
Negative_regulation (inhibition) of cathepsin B associated with cancer
8) Confidence 0.07 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.59 Pain Relevance 0
We also used MMP-9/uPAR and MMP-9/cathepsin B bicistronic constructs to evaluate the cumulative effects.
Negative_regulation (used) of cathepsin B
9) Confidence 0.07 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2904700 Disease Relevance 0.59 Pain Relevance 0
To further confirm the role of p27Kip1 in growth arrest induced by the depletion of cathepsin B and uPAR, we knocked down the expression of p27Kip1 alone or in combination with uPAR and/or cathepsin B, and we analyzed cell proliferation using BrdU incorporation assay.
Negative_regulation (depletion) of cathepsin B
10) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0 Pain Relevance 0
Cell proliferation analysis by BrdU incorporation assay showed that the depletion of cathepsin B and uPAR individually and simultaneously resulted in a significant reduction in the proliferation rates by: 37–40% (pU), 34–36% (pC) and 67–68% (pCU) in both cell lines (Fig. 1B).
Negative_regulation (depletion) of cathepsin B
11) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.06 Pain Relevance 0
In the present study, we show that the co-depletion of cathepsin B and uPAR arrests cells in the G1 phase primarily through the upregulation of p27Kip1 and that this pathway involves the downregulation of p-PI3K, p-AKT, D-type cyclin expression, and cyclin E/Cdk2 complex formation as well as the subsequent upregulation of the FOXO3a protein and its nuclear localization.
Negative_regulation (depletion) of cathepsin B
12) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.70 Pain Relevance 0
Cathepsin B and uPAR depletion affects the p27Kip1 expression and its subcellular localization
Negative_regulation (depletion) of Cathepsin B
13) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.10 Pain Relevance 0
Taken together, our findings provide molecular mechanism for the G0/G1 arrest induced by the downregulation of cathepsin B and uPAR in SNB19 and U251 glioma cells.



Negative_regulation (downregulation) of cathepsin B associated with glioma
14) Confidence 0.05 Published 2010 Journal PLoS ONE Section Abstract Doc Link PMC2908539 Disease Relevance 0.17 Pain Relevance 0
Since we observed p27Kip1 upregulation with decreased cell proliferation and G0/G1 phase arrest with the depletion of cathepsin B and uPAR, we next determined the expression of FOXO proteins, which are important transcriptional regulators of the p27Kip1 promoter.
Negative_regulation (depletion) of cathepsin B
15) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0 Pain Relevance 0
Here, we show that shRNA-mediated downregulation of cathepsin B and uPAR results in G0/G1 arrest, prominent increased expression of p27Kip1 and inhibition of p-Rb.
Negative_regulation (downregulation) of cathepsin B
16) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.24 Pain Relevance 0
In the present study, we have shown that pU, pC and pCU treatments reduced endogenous levels of cathepsin B and uPAR proteins in SNB19 and U251 glioma cells (Fig. 1A) with a 75–78% transformation efficiency as obtained using GFP (Fig.
Negative_regulation (reduced) of cathepsin B associated with glioma
17) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.53 Pain Relevance 0
In an attempt to elucidate the roles of cathepsin B and uPAR in cell cycle progression, we analyzed the activity of crucial regulators of the G0/G1 transition including p27Kip1 by downregulating cathepsin B and uPAR both individually and simultaneously in SNB19 and U251 glioma cells.
Negative_regulation (downregulating) of cathepsin B associated with glioma
18) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.36 Pain Relevance 0
In this study, we have shown that downregulation of cathepsin B and uPAR significantly decreased the dimer formation of ?
Negative_regulation (downregulation) of cathepsin B
19) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.34 Pain Relevance 0
We have previously shown that RNAi-mediated downregulation of cathepsin B and uPAR led to decreased invasion, induction of angiogenesis, increased caspase-mediated apoptosis, and induction of G0/G1 arrest [2], [30], [33]–[37] Data from other reports indicate that inhibition or depletion of cathepsin B prevents cells from entering and leaving the cell cycle, thereby decreasing cell proliferation [38], [39].
Negative_regulation (downregulation) of cathepsin B associated with apoptosis
20) Confidence 0.05 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2908539 Disease Relevance 0.70 Pain Relevance 0

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