INT337882

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Context Info
Confidence 0.76
First Reported 2010
Last Reported 2010
Negated 4
Speculated 3
Reported most in Body
Documents 1
Total Number 78
Disease Relevance 3.45
Pain Relevance 4.60

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

nucleus (Nr2f2) DNA binding (Nr2f2)
Anatomy Link Frequency
neurons 18
hypothalamus 11
hepatocytes 5
liver 4
MC3 4
Nr2f2 (Mus musculus)
Pain Link Frequency Relevance Heat
agonist 780 99.82 Very High Very High Very High
Intracerebroventricular 1014 99.12 Very High Very High Very High
Central nervous system 234 98.84 Very High Very High Very High
bDMF 468 95.08 Very High Very High Very High
Neuropeptide 234 90.36 High High
antagonist 78 63.20 Quite High
tolerance 78 59.20 Quite High
ketamine 78 5.00 Very Low Very Low Very Low
Paracetamol 78 5.00 Very Low Very Low Very Low
Disease Link Frequency Relevance Heat
Diabetes Mellitus 156 94.88 High High
Hypoglycemia 234 93.88 High High
Obesity 468 92.48 High High
Targeted Disruption 78 92.16 High High
Hyperphagia 156 88.36 High High
Hyperglycemia 78 83.20 Quite High
Repression 156 78.76 Quite High
Impaired Glucose Tolerance 78 59.84 Quite High
Weight Loss 78 59.36 Quite High
Metabolic Disorder 78 47.48 Quite Low

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
We addressed the possible overlap between COUP-TFII and SF1 expression within the VMH with a double immunostaining for both proteins.
Gene_expression (expression) of COUP-TFII
1) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
Taken altogether, these results suggested that the changes in hypothalamic COUP-TFII expression could be centrally regulated by signals of energy supplies such as blood glucose or plasma insulin levels.
Gene_expression (expression) of COUP-TFII in blood
2) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0.03
Therefore, we did a study of COUP-TFII protein expression profile by immunohistochemistry on cryostat sections of C57bl/6J fed mice brains (figure 4).
Gene_expression (expression) of COUP-TFII protein in brains
3) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
Interestingly, not all VMH cells were expressing COUP-TFII (figure 5B, green fluorescence).
Gene_expression (expressing) of COUP-TFII
4) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
So in vivo, an activation of the MC3/4-R could affect COUP-TFII gene expression directly through a transcriptional mechanism regardless of the peripheral effect of MTII on the other nuclei.
Gene_expression (expression) of COUP-TFII gene in MC3
5) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0.08
Throughout all hypothalamic sections, we detected the COUP-TFII protein only in the ventromedial nuclei (VMH) with the very characteristic perinuclear profile (figure 4B–H).
Gene_expression (detected) of COUP-TFII protein
6) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
Previous studies from our lab and from others had observed an expression of COUP-TFII mRNA in embryonic and adult hypothalamus [12], [13], [14].
Gene_expression (expression) of COUP-TFII mRNA in hypothalamus
7) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
In order to decipher the mechanisms by which insulin is able to stimulate hypothalamic COUP-TFII gene expression, we needed to identify precisely the nuclei expressing this transcription factor.
Gene_expression (expression) of COUP-TFII gene
8) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
In order to delineate if COUP-TFII was more specifically expressed in neurons or astrocytes, we undertook a co-localization study with an astrocyte specific marker, the Glial Fibrilary Acidic Protein (GFAP).
Gene_expression (expressed) of COUP-TFII in astrocyte
9) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
This led us to hypothesize that, in vivo, the fed state induced COUP-TFII expression through a local activation of the melanocortin pathway in the VMH.


Gene_expression (expression) of COUP-TFII
10) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0.25
However, some SF1 cells were not expressing COUP-TFII (white arrows), suggesting that we had at least two distinct cellular subpopulations of SF1 neurons within the VMH, the COUP-TFII positive and the COUP-TFII negative neurons with 65% of the SF1 positive cells expressing COUP-TFII (figure 5E).
Neg (not) Gene_expression (expressing) of COUP-TFII in neurons
11) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
No overlapping expression with COUP-TFII protein was observed, suggesting that COUP-TFII was not expressed in astrocytes (data not shown).
Neg (not) Gene_expression (expressed) of COUP-TFII in astrocytes
12) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
Our previous studies had shown that insulin was down-regulating COUP-TFII gene expression in liver and pancreas.
Gene_expression (expression) of COUP-TFII gene in pancreas
13) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0.07
Therefore, we questioned how the fed state could have such an opposite effect on COUP-TFII mRNA expression in the hypothalamus compared to the liver and the pancreas.


Gene_expression (expression) of COUP-TFII mRNA in pancreas
14) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
We therefore investigated the effect of insulin or of a MC3/4-R agonist on hypothalamic COUP-TFII gene expression in a hypothalamic mouse cell line, the GT1-7 cells, known to express the insulin receptor, the COUP-TFII protein (data not shown) as well as the MC4-R receptor [29].First, the cells were cultured for 18 hours with 100 nM insulin.
Gene_expression (expression) of COUP-TFII gene in MC3 associated with agonist
15) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0.08 Pain Relevance 0.12
In this paper, we report a COUP-TFII gene expression restricted to a subpopulation of neurons in the ventromedial hypothalamus expressing the SF1 protein and described as expressing the melanocortin receptor MC4-R [22], [23], [24], [25].
Gene_expression (expression) of COUP-TFII gene in neurons
16) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0.27 Pain Relevance 0.03
Under these experimental conditions, a higher expression of COUP-TFII mRNA in the fed mice than in the fasted mice was observed, therefore the ICV per se did not disturb the previously observed COUP-TFII mRNA regulation by the nutritional status (figure 7C).
Gene_expression (expression) of COUP-TFII mRNA associated with intracerebroventricular
17) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0.39
However, some SF1 cells were not expressing COUP-TFII (white arrows), suggesting that we had at least two distinct cellular subpopulations of SF1 neurons within the VMH, the COUP-TFII positive and the COUP-TFII negative neurons with 65% of the SF1 positive cells expressing COUP-TFII (figure 5E).
Gene_expression (expressing) of COUP-TFII in neurons
18) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
Hypothalamic COUP-TFII expression is induced by the fed state in adult mice
Gene_expression (expression) of COUP-TFII
19) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0 Pain Relevance 0
In vivo, the observed induction of COUP-TFII gene expression by the fed state could be therefore mediated indirectly by insulin through the conjunction of decreasing amount of AgRP and increasing amount of POMC leading downstream to an activation of the MC3/4-R.
Gene_expression (expression) of COUP-TFII gene in MC3
20) Confidence 0.76 Published 2010 Journal PLoS ONE Section Body Doc Link PMC2956692 Disease Relevance 0.06 Pain Relevance 0.14

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