INT346248

Context Info Confidence 0.47 First Reported 2010 Last Reported 2010 Negated 0 Speculated 0 Reported most in Body Documents 1 Total Number 3 Disease Relevance 1.73 Pain Relevance 0.78

cytosol (MAP2K3) nucleoplasm (MAP2K3) signal transduction (MAP2K3)

Anatomy Link Frequency chondrocytes 6 MAP2K3 (Homo sapiens)

Pain Link Frequency Relevance Heat Osteoarthritis 309 100.00 imagery 3 39.80 Immobilon 3 20.12 Inflammation 48 5.00 cytokine 24 5.00 Arthritis 24 5.00 metalloproteinase 18 5.00 rheumatoid arthritis 12 5.00 Pain 6 5.00 Inflammatory stimuli 3 5.00

Disease Link Frequency Relevance Heat Osteoarthritis 312 100.00 Targeted Disruption 9 95.80 INFLAMMATION 42 5.00 Arthritis 27 5.00 Disease 15 5.00 Rheumatoid Arthritis 12 5.00 Pain 3 5.00 Apoptosis 3 5.00 Hypertrophy 3 5.00 Frailty 3 5.00

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Expression and activation of MKK3 and MKK6 in human OA chondrocytes

Positive_regulation (activation) of Gene_expression (Expression) of MKK3 in chondrocytes associated with osteoarthritis

1) Confidence 0.47 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.43 Pain Relevance 0.22

Human OA chondrocytes were transfected with human MKK3 or MKK6-specific siRNAs using On-Target SMART Pools (# L-00350900, L-00396700, Dharmacon RNA Technologies, Lafayette, CO, USA).

Positive_regulation (transfected) of Gene_expression (transfected) of MKK3 in chondrocytes associated with osteoarthritis

2) Confidence 0.47 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 0.22 Pain Relevance 0.11

Transfection of human OA chondrocytes with MKK3 or MKK6-specific siRNAs knocked down the expression of target proteins and inhibited the IL-1?

Positive_regulation (Transfection) of Gene_expression (Transfection) of MKK3 in chondrocytes associated with osteoarthritis

3) Confidence 0.47 Published 2010 Journal Arthritis Res Ther Section Body Doc Link PMC2991031 Disease Relevance 1.08 Pain Relevance 0.45

General Comments

This test has worked.