INT40992

Context Info Confidence 0.46 First Reported 1982 Last Reported 1983 Negated 0 Speculated 0 Reported most in Abstract Documents 2 Total Number 4 Disease Relevance 0 Pain Relevance 2.33

Golgi apparatus (Xrn1) RNA binding (Xrn1) plasma membrane (Xrn1) intracellular (Xrn1) DNA binding (Xrn1) cytoplasm (Xrn1)

Xrn1 (Mus musculus)

Pain Link Frequency Relevance Heat Dynorphin 29 99.56 Morphine 6 99.10 Enkephalin 3 99.00 Opioid 1 97.50 tail-flick 1 93.44 opiate 6 92.72 analgesia 1 85.52 Analgesic 1 80.72

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Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Binding assays indicated that two opioid peptides had similar profiles in that they enhanced dihydromorphine (DHM) binding in picomolar concentrations but displaced DHM binding in nanomolar concentrations (IC50 for Dynorphin(1-10)amide = 5 nM).

DHM Binding (binding) of associated with dynorphin and opioid

1) Confidence 0.46 Published 1982 Journal Life Sci. Section Abstract Doc Link 6130447 Disease Relevance 0 Pain Relevance 0.98

Dynorphin pretreatment was found to decrease DHM binding in vitro in a both time-dependent and dose-dependent manner.

DHM Binding (binding) of associated with dynorphin

2) Confidence 0.30 Published 1983 Journal Eur. J. Pharmacol. Section Abstract Doc Link 6311575 Disease Relevance 0 Pain Relevance 0.43

Extra washes of tissue did not significantly increase the binding, but preincubation of tissue in the presence of morphine reversed the dynorphin effect and increased DHM binding significantly to almost control levels.

DHM Binding (binding) of associated with dynorphin and morphine

3) Confidence 0.20 Published 1983 Journal Eur. J. Pharmacol. Section Abstract Doc Link 6311575 Disease Relevance 0 Pain Relevance 0.58

Mice were injected intracerebroventricularly with different doses of dynorphin-(1-13) in vivo and decapitated after different intervals of time; crude P2 fractions were then prepared and used for in vitro binding with [3H]dihydromorphine (DHM) and [3H][D-Ala2,D-Leu5]enkephalin.

DHM Binding (binding) of associated with dynorphin and enkephalin

4) Confidence 0.20 Published 1983 Journal Eur. J. Pharmacol. Section Abstract Doc Link 6311575 Disease Relevance 0 Pain Relevance 0.35

General Comments

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