INT73593

Context Info Confidence 0.76 First Reported 1998 Last Reported 2010 Negated 4 Speculated 6 Reported most in Body Documents 173 Total Number 181 Disease Relevance 147.81 Pain Relevance 20.94

lipid binding (BAX) cytosol (BAX) mitochondrion (BAX) endoplasmic reticulum (BAX) nucleus (BAX) cytoplasm (BAX)

Anatomy Link Frequency MCF-7 7 cleavage 3 apoptotic cells 3 endometrium 3 MDA-MB-231 3 BAX (Homo sapiens)

Pain Link Frequency Relevance Heat antagonist 110 99.96 Morphine 55 99.84 Inflammation 287 99.80 Paracetamol 11 99.78 cINOD 157 99.70 Dismenorea 17 99.56 endometriosis 231 98.56 palliative 37 98.28 diclofenac 11 97.96 Chronic pancreatitis 20 97.92

Disease Link Frequency Relevance Heat Apoptosis 5425 100.00 Death 1082 100.00 Endometriosis 260 100.00 Multiple Endocrine Neoplasia Type 1 1 100.00 Cancer 3133 99.98 Obesity 411 99.98 Microsatellite Instability 52 99.96 Lung Cancer 755 99.84 Pancreatic Cancer 638 99.84 INFLAMMATION 376 99.80

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Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

OBJECTIVE: To determine the Bcl-2 and Bax expressions in endometriotic and adenomyotic tissues.

Spec (determine) Gene_expression (expressions) of Bax associated with endometriosis and dismenorea

1) Confidence 0.76 Published 2001 Journal Eur. J. Obstet. Gynecol. Reprod. Biol. Section Abstract Doc Link 11788183 Disease Relevance 0.35 Pain Relevance 0.17

Bcl-2 and Bax expression in human endometriotic and adenomyotic tissues.

Gene_expression (expression) of Bax associated with endometriosis and dismenorea

2) Confidence 0.76 Published 2001 Journal Eur. J. Obstet. Gynecol. Reprod. Biol. Section Title Doc Link 11788183 Disease Relevance 0.39 Pain Relevance 0.19

Bcl-2 and Bax expressions were investigated on 56 formalin-fixed and paraffin-embedded tissue by immunohistochemical staining and electron microscopy.

Spec (investigated) Gene_expression (expressions) of Bax

3) Confidence 0.76 Published 2001 Journal Eur. J. Obstet. Gynecol. Reprod. Biol. Section Body Doc Link 11788183 Disease Relevance 0 Pain Relevance 0

RESULTS: In normal pancreas and chronic pancreatitis tissues, bcl-2, bax and bcl-x were predominantly expressed in ductal epithelial cells while p53 was not detected.

Gene_expression (expressed) of bax in epithelial cells

4) Confidence 0.75 Published 2001 Journal Pancreatology Section Body Doc Link 12120204 Disease Relevance 0.08 Pain Relevance 0

Detailed tissue expression of bcl-2, bax, bak and bcl-x in the normal human pancreas and in chronic pancreatitis, ampullary and pancreatic ductal adenocarcinomas.

Gene_expression (expression) of bax in pancreas associated with adenocarcinoma and chronic pancreatitis

5) Confidence 0.75 Published 2001 Journal Pancreatology Section Title Doc Link 12120204 Disease Relevance 0.19 Pain Relevance 0.10

We observed that IR-induced bax and p21(WAF1/Cip1) protein expression were attenuated selectively in normal stromal and epithelial cell cultures, yet maintained their p53-dependency in malignant cell lines.

Gene_expression (expression) of bax in epithelial cell associated with malignant neoplastic disease

6) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.91 Pain Relevance 0.10

The expression of p53, p21, bax and caspase-3 increased in Tan-I-treated cells.

Gene_expression (expression) of bax

7) Confidence 0.75 Published 2008 Journal Int. J. Mol. Med. Section Abstract Doc Link 18949381 Disease Relevance 1.00 Pain Relevance 0.08

The expression of bcl-2 and bax was analyzed by Western blot analysis.

Spec (analyzed) Gene_expression (expression) of bax

8) Confidence 0.75 Published 2008 Journal Exp. Oncol. Section Body Doc Link 19112426 Disease Relevance 0.08 Pain Relevance 0

To clarify the basis for cell killing during prostate cancer radiotherapy, we determined the IR-induced expression of several apoptotic- (bax, bcl-2, survivin and PARP) and G1-cell cycle checkpoint- (p53 and p21(WAF1/Cip1)) related proteins, in both normal (PrEC-epithelial and PrSC-stromal) and malignant (LNCaP, DU-145 and PC-3; all epithelial) prostate cells.

Gene_expression (expression) of bax in PC-3 associated with malignant neoplastic disease, apoptosis and reprotox - general 1

9) Confidence 0.75 Published 2003 Journal Prostate Cancer Prostatic Dis. Section Abstract Doc Link 12664070 Disease Relevance 0.72 Pain Relevance 0.07

RESULTS: Our results showed that aspisol reduced viability of MDA-MB-231 cells in time- and dose- dependent fashions and induced apoptosis by increase of caspase-3 and bax expressions while decrease of COX-2 and bcl-2 expression in vitro.

Gene_expression (expressions) of bax in MDA-MB-231

10) Confidence 0.75 Published 2008 Journal Exp. Oncol. Section Body Doc Link 19112426 Disease Relevance 0.07 Pain Relevance 0

CONCLUSIONS: Our in vitro and in vivo data indicated that the antitumor effects of aspisol on breast cancer cells was probably mediated by the induction of apoptosis, and it could be linked to the downregulation of the COX-2 or bcl-2 expression and up-regulation of caspase-3 or bax expression.

Gene_expression (expression) of bax

11) Confidence 0.75 Published 2008 Journal Exp. Oncol. Section Body Doc Link 19112426 Disease Relevance 0 Pain Relevance 0

Ribonuclease protection assays compared the effects of acetaminophen and E2 on expression of selected genes (c-myc, c-fos, cyclin D1, bcl-2, bax, gadd45, mcl-1, p53, p21(CIP1/WAF1), and bcl-xL) in E2-responsive breast cancer (MCF-7) and endometrial adenocarcinoma (Ishikawa) cells as well as in E2-nonresponsive (MDA-MB-231) breast cancer cells.

Gene_expression (expression) of bax in MDA-MB-231 associated with paracetamol, endometriosis (extended) and breast cancer

12) Confidence 0.75 Published 2002 Journal Toxicol. Sci. Section Abstract Doc Link 11896290 Disease Relevance 0.36 Pain Relevance 0.55

Expression of cyclins B1, D3 and E and p21, p27, bax, bcl2 and cox-2 was studied immunohistochemically.

Gene_expression (Expression) of bax

13) Confidence 0.75 Published 2007 Journal Eur. J. Cancer Section Abstract Doc Link 17434727 Disease Relevance 0.62 Pain Relevance 0.07

However the expression of apoptosis-related genes such as Fas, bcl-2 and bax was not affected by indomethacin.

Gene_expression (expression) of bax associated with apoptosis

14) Confidence 0.75 Published 1998 Journal Yonsei Med. J. Section Abstract Doc Link 9752793 Disease Relevance 1.45 Pain Relevance 0.95

This study focused on the biological mechanisms of sensitivity and resistance to NCX 4040, highlighting that the cytotoxic action of this drug may be due to the hyperexpression of Bax, its translocation to the mitochondria, the release of Cytochrome C, and the activation of caspases-9 and -3, overall in a p53-independent manner.

Gene_expression (hyperexpression) of Bax

15) Confidence 0.75 Published 2006 Journal Apoptosis Section Abstract Doc Link 16699954 Disease Relevance 0.31 Pain Relevance 0.48

Morphine-treated Jurkat cells also showed a decreased expression of bcl-2 and an enhanced expression of bax.

Gene_expression (expression) of bax associated with morphine

16) Confidence 0.75 Published 1999 Journal J. Leukoc. Biol. Section Abstract Doc Link 10534122 Disease Relevance 0.94 Pain Relevance 0.87

Since Bax expression was stimulated only at high resveratrol concentrations while Bax translocation and mitochondria-mediated cell death were observed also at low concentrations, we asked what relevance Bax might have for the chemopreventive activity of the compound.

Gene_expression (expression) of Bax associated with death

17) Confidence 0.74 Published 2002 Journal BMC Cancer Section Body Doc Link PMC130964 Disease Relevance 1.14 Pain Relevance 0.35

In other model systems, Bax was overproduced in response to drugs, including resveratrol [10,14].

Gene_expression (overproduced) of Bax

18) Confidence 0.74 Published 2002 Journal BMC Cancer Section Body Doc Link PMC130964 Disease Relevance 1.24 Pain Relevance 0.37

Concomitantly to the absence of apoptosis in those endometria, the pro-apoptotic gene bax mRNA abundance was unchanged during the menstrual cycle, at difference at the increase exhibited in normal endometrium, suggesting that the reduction of bax expression may be a mechanism that could explain the decreased incidence of apoptosis in endometriosis.

Gene_expression (expression) of bax in endometrium associated with endometriosis and apoptosis

19) Confidence 0.74 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1262771 Disease Relevance 1.26 Pain Relevance 0.22

In agreement with these results, recent publications [3,34,35] also reported the absence of apoptotic cells and the increase of Bcl-2 and the reduction of Bax expression in eutopic endometrium from women with endometriosis, although Béliard et al. (2004) [4] were unable to find these differences between patients with endometriosis and normal women.

Gene_expression (expression) of Bax in endometrium associated with endometriosis and apoptosis

20) Confidence 0.74 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1262771 Disease Relevance 1.28 Pain Relevance 0.22

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