INT8336

Context Info Confidence 0.71 First Reported 1989 Last Reported 2010 Negated 1 Speculated 1 Reported most in Abstract Documents 22 Total Number 24 Disease Relevance 4.37 Pain Relevance 4.69

extracellular space (Gnrh1) extracellular region (Gnrh1) molecular_function (Gnrh1)

Anatomy Link Frequency brain 2 neurons 2 plasma 1 lateral preoptic area 1 lactotroph 1 Gnrh1 (Rattus norvegicus)

Pain Link Frequency Relevance Heat monoamine 5 100.00 narcan 4 99.92 antagonist 87 99.74 opiate 12 99.34 Morphine 7 99.06 Opioid 9 98.78 noradrenaline 14 97.36 Catecholamine 1 97.12 tetrodotoxin 2 95.76 palliative 2 94.96

Disease Link Frequency Relevance Heat Precocious Puberty 215 98.80 Urological Neuroanatomy 34 94.08 Starvation 30 93.04 Reprotox - General 1 13 93.04 Osteoporosis 1 92.88 Hyponatremia 1 90.04 Body Weight 6 89.76 Disease 30 89.44 Depression 5 88.60 Targeted Disruption 44 88.56

Sentences Mentioned In

Key:

Protein

Mutation

Event

Anatomy

Negation

Speculation

Pain term

Disease term

Thus, it seems that stimuli which evoke LHRH release also increase LHRH mRNA transcription to replenish the hormone released during the LH surge.

Transcription (transcription) of LHRH mRNA

1) Confidence 0.71 Published 1992 Journal Ciba Found. Symp. Section Abstract Doc Link 1425026 Disease Relevance 0.08 Pain Relevance 0.62

The other half of the brain in Experiment 2 was used to investigate the expression of GnRH mRNA by RT-PCR.

Spec (investigate) Transcription (expression) of GnRH in brain

2) Confidence 0.71 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1236957 Disease Relevance 0 Pain Relevance 0

In the present study, the high level of GnRH mRNA expression was observed in the M, but the number of the GnRH neuron decreased.

Transcription (expression) of GnRH in neuron

3) Confidence 0.71 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1236957 Disease Relevance 0.32 Pain Relevance 0.04

Effects of herb mixture on GnRH mRNA expression by RT-PCR

Transcription (expression) of GnRH

4) Confidence 0.71 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1236957 Disease Relevance 0.06 Pain Relevance 0

Nevertheless, our results, the GnRH mRNA expression showed a higher level in the precocious puberty model rats than in the same age normal rats, the blood estrogen concentration in the model group is twofold higher than that in normal one, along with the day of vaginal opening and the onset of regular estrous cycle advanced significantly in the model rats, give more evidence to sustain the hypothesis that the model induced by neonatal treatment with Danazol is a true precocious puberty model, and might be served to study the onset mechanism of puberty.

Transcription (expression) of GnRH in blood associated with precocious puberty

5) Confidence 0.71 Published 2005 Journal Reprod Biol Endocrinol Section Body Doc Link PMC1236957 Disease Relevance 0.46 Pain Relevance 0.03

To investigate this issue, we used dual label in situ hybridization histochemistry to determine whether LHRH neurons synthesize messenger RNA (mRNA) for mu, kappa, and/or delta opiate receptors.

Transcription (synthesize) of LHRH in neurons associated with opiate

6) Confidence 0.69 Published 1997 Journal Endocrinology Section Abstract Doc Link 9075729 Disease Relevance 0 Pain Relevance 0.33

NMDA rapidly releases LH and LHRH mRNA levels are significantly raised within 15 minutes and remain so over the next 45 minutes.

Transcription (raised) of LHRH mRNA

7) Confidence 0.69 Published 1992 Journal Ciba Found. Symp. Section Abstract Doc Link 1425026 Disease Relevance 0.09 Pain Relevance 0.74

This review provides an update on leuprorelin acetate, the world's most widely prescribed depot luteinising hormone-releasing hormone analogue.

Transcription (prescribed) of luteinising hormone-releasing hormone

8) Confidence 0.67 Published 2002 Journal Int. J. Clin. Pract. Section Abstract Doc Link 12137449 Disease Relevance 0.23 Pain Relevance 0.09

As the technical parameters and particularly the tissue fixation used to detect neutral endopeptidase are compatible with immunocytochemical detection of GnRH and tyrosine-hydroxylase (the rate limiting enzyme in the synthesis of catecholamines), two double immunolabelings were realised at the ultrastructural level to determine if GnRH and dopamine nerve endings have the enzyme inserted within their plasma membrane.

Transcription (detection) of GnRH in plasma associated with dopamine and catecholamine

9) Confidence 0.66 Published 1994 Journal J. Neuroendocrinol. Section Abstract Doc Link 7894468 Disease Relevance 0 Pain Relevance 0.50

As a basis for a rapid fall in peptide secretion, we hypothesize that LHRH mRNA levels rapidly decay.

Transcription (levels) of LHRH

10) Confidence 0.53 Published 1997 Journal J. Neurosci. Section Abstract Doc Link 9391004 Disease Relevance 0 Pain Relevance 0.10

A plot of LHRH mRNA level per cell versus DRB treatment time showed a rapid initial decay of LHRH mRNA (t1/2, 5-13 min), followed by a slower decay rate (t1/2, 329-344 hr).

Transcription (level) of LHRH

11) Confidence 0.53 Published 1997 Journal J. Neurosci. Section Abstract Doc Link 9391004 Disease Relevance 0 Pain Relevance 0.15

We tested this hypothesis by comparing cellular levels of GnRH mRNA between ad libitum fed (n = 4) and starved (n = 4) adult male rats.

Transcription (levels) of GnRH

12) Confidence 0.53 Published 1992 Journal Neuroendocrinology Section Abstract Doc Link 1369602 Disease Relevance 0.33 Pain Relevance 0.06

We reasoned that if dietary restriction reduces GnRH secretion, this would be reflected by a decrease in GnRH synthesis and likewise cellular GnRH mRNA levels.

Transcription (levels) of GnRH

13) Confidence 0.53 Published 1992 Journal Neuroendocrinology Section Abstract Doc Link 1369602 Disease Relevance 0.32 Pain Relevance 0.05

The GnRH mRNA expression increased significantly in M compared with that in N and V (p < 0.05).

Transcription (expression) of GnRH

14) Confidence 0.53 Published 2005 Journal Reprod Biol Endocrinol Section Abstract Doc Link PMC1236957 Disease Relevance 0.41 Pain Relevance 0

We detected no significant change with puberty in GnRH mRNA signal levels in any of the anatomical areas examined, which included the vertical limb of the diagonal band of Broca, medial septum, lateral preoptic area, and medial preoptic area.

Transcription (levels) of GnRH in lateral preoptic area associated with urological neuroanatomy

15) Confidence 0.53 Published 1989 Journal Endocrinology Section Abstract Doc Link 2647464 Disease Relevance 0.09 Pain Relevance 0.19

In rats, GnRH and GnRH receptor mRNA has been found in the myenteric plexus [20,21].

Transcription (found) of GnRH

16) Confidence 0.51 Published 2010 Journal BMC Gastroenterol Section Body Doc Link PMC2885307 Disease Relevance 0.24 Pain Relevance 0.08

In rats, GnRH and GnRH receptor mRNA has been found in the myenteric plexus [20,21].

Transcription (found) of GnRH

17) Confidence 0.51 Published 2010 Journal BMC Gastroenterol Section Body Doc Link PMC2885307 Disease Relevance 0.25 Pain Relevance 0.08

In order to investigate the role of opiates in the regulation of GnRH gene expression in the rat brain, we studied the effects of chronic administration of the opioid drug morphine and an opiate receptor antagonist naloxone on GnRH mRNA levels as measured by in situ hybridization.

Transcription (levels) of GnRH in brain associated with antagonist, narcan, opiate, opioid and morphine

18) Confidence 0.51 Published 1993 Journal Neuroreport Section Abstract Doc Link 8477057 Disease Relevance 0 Pain Relevance 1.13

We have shown that GnRH mRNA is expressed in Rathke's pouch explants (106).

Transcription (expressed) of GnRH in Rathke's pouch

19) Confidence 0.38 Published 2008 Journal Journal of Neuroendocrinology Section Body Doc Link PMC2229370 Disease Relevance 0 Pain Relevance 0.07

The mechanism of that early action, however, remains unknown, but other factors seem to compensate the absence of GnRH, indicating the robustness of the lactotroph developmental track.

Neg (absence) Transcription (absence) of GnRH in lactotroph

20) Confidence 0.36 Published 2008 Journal Journal of Neuroendocrinology Section Body Doc Link PMC2229370 Disease Relevance 0.17 Pain Relevance 0.04

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