INT85640

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Context Info
Confidence 0.72
First Reported 2000
Last Reported 2011
Negated 29
Speculated 23
Reported most in Body
Documents 1138
Total Number 1166
Disease Relevance 311.58
Pain Relevance 119.18

This is a graph with borders and nodes. Maybe there is an Imagemap used so the nodes may be linking to some Pages.

Anatomy Link Frequency
neurons 135
retina 22
bone marrow 20
brain 16
eye 15
Tg(CAG-EGFP)D4Nagy (Mus musculus)
Pain Link Frequency Relevance Heat
Spinal cord 4993 100.00 Very High Very High Very High
gABA 1025 100.00 Very High Very High Very High
Somatosensory cortex 238 100.00 Very High Very High Very High
Nav1.7 102 100.00 Very High Very High Very High
narcan 86 100.00 Very High Very High Very High
GABAergic 1688 99.98 Very High Very High Very High
nMDA receptor 1483 99.98 Very High Very High Very High
Pyramidal cell 1640 99.96 Very High Very High Very High
endometriosis 1152 99.96 Very High Very High Very High
substantia gelatinosa 42 99.96 Very High Very High Very High
Disease Link Frequency Relevance Heat
Cancer 12345 100.00 Very High Very High Very High
Targeted Disruption 7391 100.00 Very High Very High Very High
Cytomegalovirus Infection 2743 100.00 Very High Very High Very High
Sprains And Strains 2495 100.00 Very High Very High Very High
Infection 5136 99.98 Very High Very High Very High
Endometriosis 1332 99.96 Very High Very High Very High
Pancreatic Cancer 160 99.96 Very High Very High Very High
Skin Cancer 244 99.86 Very High Very High Very High
Ebola Virus Infection 225 99.86 Very High Very High Very High
Carcinoma 180 99.86 Very High Very High Very High

Sentences Mentioned In

Key: Protein Mutation Event Anatomy Negation Speculation Pain term Disease term
The expression of GFP reporter gene was observable and traceable in epidermis and spleen over 3 days.
Gene_expression (expression) of GFP in spleen
1) Confidence 0.72 Published 2009 Journal Biomaterials Section Abstract Doc Link 19656560 Disease Relevance 0.42 Pain Relevance 0.21
The expressions of GFP and the activation of dendritic cells (DCs) were evident and co-localized in hair follicles and epidermis.
Gene_expression (expressions) of GFP in hair follicles
2) Confidence 0.72 Published 2009 Journal Biomaterials Section Abstract Doc Link 19656560 Disease Relevance 0.40 Pain Relevance 0.21
Notably, LV-EGFP administration and EGFP overexpression in glial cells did neither modify glial activity, nor alter animal's nociceptive or locomotor behaviors.
Gene_expression (overexpression) of EGFP in glial cells associated with nociception
3) Confidence 0.71 Published 2008 Journal J. Neurosci. Methods Section Abstract Doc Link 17949823 Disease Relevance 0.47 Pain Relevance 0.48
EGFP transgene was mainly expressed in astrocytes and microglial cells whereas less than 9% of cells containing EGFP were neurons.
Gene_expression (expressed) of EGFP in microglial cells
4) Confidence 0.71 Published 2008 Journal J. Neurosci. Methods Section Abstract Doc Link 17949823 Disease Relevance 0.48 Pain Relevance 0.48
Single microinjection of vector expressing EGFP under a CMV promoter control (LV-EGFP) allowed vector diffusion along a rostro-caudal axis but strictly restricted to the grey matter of the ipsilateral dorsal spinal cord.
Gene_expression (expressing) of EGFP in spinal cord associated with cytomegalovirus infection and spinal cord
5) Confidence 0.71 Published 2008 Journal J. Neurosci. Methods Section Abstract Doc Link 17949823 Disease Relevance 0.49 Pain Relevance 0.44
In summary, we found that GFP can be introduced into the anterior segment of the Balb/c mouse via a single IVT or IC injection of Ad5.CMV-GFP.
Gene_expression (introduced) of GFP in anterior associated with cytomegalovirus infection
6) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.18 Pain Relevance 0
The intensity and duration of GFP expression were dependent on the injection route, viral titer, and treatment with antiCD40L antibody.
Gene_expression (expression) of GFP
7) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.16 Pain Relevance 0
This observation was confirmed by analysis of the data with three-way ANOVA, which indicated that GFP expression was significantly higher in the anti-CD40L than the no antibody group (p=0.013), significantly higher in the IVT than the IC group (p=0.003), and significantly higher in the high viral titer (1x108 pfu) than the low titer (1x107 pfu) group (p=0.010).
Gene_expression (expression) of GFP
8) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.13 Pain Relevance 0
IC injection at a higher titer of the vector (1x108 pfu), even without anti-CD40L antibody treatment, produced more noticeable GFP expression.
Gene_expression (expression) of GFP
9) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.22 Pain Relevance 0
Again, treatment with anti-CD40L antibody augmented the expression of GFP.
Gene_expression (expression) of GFP
10) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.29 Pain Relevance 0
The expression of GFP can be easily monitored by the green fluorescence.
Gene_expression (expression) of GFP
11) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.47 Pain Relevance 0.09
Cross-sections of mouse eyes obtained from animals euthanized at days 5 and 43 following intraocular Ad5.CMV-GFP injection were examined by fluorescence microscopy, and GFP expression was typically localized to the cornea, TM, iris, ciliary body, and lens.
Gene_expression (expression) of GFP in ciliary body associated with cytomegalovirus infection
12) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.28 Pain Relevance 0
We reduced this shortcoming by immunomodulation; treating the animals with the anti-CD40L antibody, which clearly increased the expression of GFP after IC injection, and significantly prolonged GFP expression after IVT injection to more than six weeks.
Gene_expression (expression) of GFP
13) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.11 Pain Relevance 0
Interestingly, when Ad5.CMV-GFP was injected intravitreally, the expression of GFP in the anterior segment was clearly higher than when it was injected intracamerally.
Gene_expression (expression) of GFP in anterior associated with cytomegalovirus infection
14) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.26 Pain Relevance 0
Immunomodulation of the animals by intraperitoneal injection of anti-CD40L antibody enhanced both the time and intensity of GFP expression.



Gene_expression (expression) of GFP
15) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.15 Pain Relevance 0
Expression of GFP: in vivo observations
Gene_expression (Expression) of GFP
16) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.09 Pain Relevance 0
E3) adenovirus serotype 5 particles expressing the GFP gene under control of the cytomegalovirus-IE promoter/enhancer.
Gene_expression (expressing) of GFP associated with cytomegalovirus infection
17) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.46 Pain Relevance 0.11
GFP was expressed in the TM, corneal endothelium, and to some extent other anterior segment tissues.
Gene_expression (expressed) of GFP in anterior
18) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.17 Pain Relevance 0
Whereas, increasing the injected viral titer to 1x108 pfu boosted GFP expression to a higher level (Fluorescence Grade at day 5=0.83 ±0.38, n=9; Figure 2B).
Gene_expression (expression) of GFP
19) Confidence 0.68 Published 2008 Journal Molecular Vision Section Body Doc Link PMC2267727 Disease Relevance 0.25 Pain Relevance 0
The silencing of the eGFP expression was significant at 48 hrs as predicted from the life time of the eGFP:24hrs (Corish and Tyler-Smith, 1999).
Gene_expression (expression) of eGFP
20) Confidence 0.66 Published 2008 Journal Journal of RNAi and Gene Silencing : An International Journal of RNA and Gene Targeting Research Section Body Doc Link PMC2737239 Disease Relevance 0 Pain Relevance 0

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